IHC image goat anti GFAP polyclonal antibody 8205

rabbit anti-VSV-G tag polyclonal antibody 9682

$100.00 – $2,600.00

Antibody summary

Rabbit polyclonal to VSV-G tag
Suitable for: ELISA
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

SKU: 9682parent Categories: antibody, epitope tag Tag: VSV-G

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-VSV-G tag polyclonal antibody 9682

antibody
Database link: VSV G tag Sequence - Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys
Tested applications ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with YTDIEMNRLGK as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen YTDIEMNRLGK (VSV-g epitope) conjugated with KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance

VSV-G tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify VSV-G tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, VSV-G tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and VSV-G tag

Biotechnology
The VSV-G tag has become a valuable and versatile tool in scientific research, particularly in molecular biology, cell biology, and virology studies. The VSV-G tag is derived from the glycoprotein of the Vesicular Stomatitis Virus (VSV), a virus that infects animals and does not replicate in mammalian cells. This feature makes the VSV-G tag highly useful for pseudotyping viral particles and creating viral vectors for gene delivery and expression studies. Researchers frequently use the VSV-G tag to replace the native envelope protein of viral vectors, enabling efficient and broad transduction of target cells, including difficult-to-transfect cell types. Furthermore, the VSV-G tag is often employed as a fusion tag for protein expression and purification, as it is well-tolerated and effectively trafficked to the plasma membrane. The VSV-G tag can be readily detected using specific antibodies in a variety of experimental assays, such as Western blotting and immunofluorescence, facilitating the visualization and quantification of tagged proteins. The widespread application of the VSV-G tag in research can be attributed to its utility in viral vector design, as well as its compatibility with multiple expression systems, making it a valuable tool for investigating cellular processes, gene function, and protein dynamics in diverse biological contexts.

Publications

pmid	title	authors	citation
32452418	Human mastadenovirus-B (HAdV-B)-specific E3-CR1β and E3-CR1γ glycoproteins interact with each other and localize at the plasma membrane of non-polarized airway epithelial cells.	Poornima Kotha Lakshmi Narayan, Adriana E Kajon	Virology 546:67-78
28259047	The tripartite leader sequence is required for ectopic expression of HAdV-B and HAdV-E E3 CR1 genes.	Camden R Bair, Poornima Kotha Lakshmi Narayan, Adriana E Kajon	Virology 505:139-147

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.