rabbit anti-VSV-G tag polyclonal antibody 9682

Price range: $100.00 through $2,600.00

Antibody summary

Rabbit polyclonal to VSV-G tag
Suitable for: ELISA
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 9682parent Categories: antibody, epitope tag Tag: VSV-G

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-VSV-G tag polyclonal antibody 9682

antibody
Database link: VSV G tag Sequence - Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys
Tested applications ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with YTDIEMNRLGK as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen YTDIEMNRLGK (VSV-g epitope) conjugated with KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
VSV-G tag
Protein names VSV-G epitope tag
Alternative names VSV-G tag, VSV epitope, Vesicular stomatitis virus glycoprotein tag, YTDIEMNRLGK tag
Function The VSV-G tag is an artificial peptide epitope used for detection and characterization of recombinant proteins using anti-VSV-G antibodies.
Description The VSV-G tag is a peptide epitope derived from the glycoprotein G of Vesicular stomatitis virus (VSV). It is commonly used as a recombinant protein tag for detection, localization, and characterization of fusion proteins.
Keywords Epitope tag, Protein detection, Immunoprecipitation, Protein localization, Recombinant protein
Sequence YTDIEMNRLGK
Structure The VSV-G tag is an 11-amino acid peptide epitope with the sequence YTDIEMNRLGK derived from the vesicular stomatitis virus glycoprotein G.
Function The VSV-G tag is used as a molecular epitope tag for detection, immunoprecipitation, purification, and localization of recombinant proteins.
Detection VSV-G-tagged proteins can be detected using anti-VSV-G monoclonal antibodies in western blotting, ELISA, immunofluorescence, immunocytochemistry, flow cytometry, and immunoprecipitation assays.
Applications The VSV-G tag is typically fused to recombinant proteins at the N- or C-terminus to facilitate detection while minimizing effects on protein function.
Research relevance The VSV-G tag is a well-established recombinant protein tag and is commonly used alongside FLAG, HA, Myc, V5, and His tags in molecular biology and biotechnology.

Data

ICC/IF with antibody [9682]

FAQ & Publications

Frequently Asked Questions

What applications is the rabbit anti-VSV-G tag polyclonal antibody 9682 suitable for?

This antibody is suitable for ELISA, blotting, and immunohistochemistry (IHC) applications. Optimal working dilutions should be determined experimentally by the investigator.

How should the rabbit anti-VSV-G tag polyclonal antibody 9682 be stored to maintain stability?

The antibody should be stored at 2-8°C in its provided buffer, which is PBS at pH 7.2 containing 0.09% sodium azide (NaN₃).

What is the immunogen used to generate the rabbit anti-VSV-G tag polyclonal antibody 9682?

The immunogen is the VSV-G epitope peptide sequence YTDIEMNRLGK conjugated to keyhole limpet hemocyanin (KLH).

Is the rabbit anti-VSV-G tag polyclonal antibody 9682 affinity purified and what is its isotype?

Yes, the antibody is affinity purified and is of the IgG isotype derived from rabbit host.

Publications

pmid	title	authors	citation
32452418	Human mastadenovirus-B (HAdV-B)-specific E3-CR1β and E3-CR1γ glycoproteins interact with each other and localize at the plasma membrane of non-polarized airway epithelial cells.	Poornima Kotha Lakshmi Narayan, Adriana E Kajon	Virology 546:67-78
28259047	The tripartite leader sequence is required for ectopic expression of HAdV-B and HAdV-E E3 CR1 genes.	Camden R Bair, Poornima Kotha Lakshmi Narayan, Adriana E Kajon	Virology 505:139-147

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.