mouse anti-DYDDDDK tag monoclonal antibody (FG4R) 5299

Price range: $100.00 through $2,600.00

Antibody summary

Mouse monoclonal to DYDDDDK tag
Suitable for: WB,ICC/IF,ELISA
Reacts with: tagged fusion proteins
Isotype: IgG1
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 5299parent Categories: antibody, epitope tag Tag: DYDDDDK tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-DYDDDDK tag monoclonal antibody (FG4R) 5299

antibody
Database link: DYDDDDK tag (FLAG tag)
Tested applications WB,ICC/IF,ELISA
Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Immunogen DYKDDDDK (FLAG) synthetic peptide conjugated to KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Storage buffer PBS, pH 7.2, 0.05% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
DDDDK
Protein names FLAG tag
Alternative names FLAG tag, FLAG epitope, DYKDDDDK tag, DYDDDDK tag, FLAG peptide
Function The FLAG tag serves as an affinity and detection tag that can be fused to recombinant proteins. It is recognized by anti-FLAG antibodies and affinity reagents for purification, immunoprecipitation, western blotting, immunofluorescence, flow cytometry, and ELISA.
Description The FLAG tag is a short artificial peptide epitope widely used in recombinant protein expression, purification, detection, and localization studies. The canonical FLAG sequence is DYKDDDDK.
Biological role Recombinant protein detection Affinity purification Immunoprecipitation Protein localization studies
Research relevance FLAG-tagged proteins are extensively used in molecular biology and protein expression systems because the tag is small, highly immunogenic, and generally does not interfere with protein folding or function.
Keywords Epitope tag, Protein purification, Protein detection, Recombinant protein
Structure The FLAG tag is a synthetic peptide epitope with the canonical amino acid sequence DYKDDDDK.
Function FLAG serves as a molecular tag for detection, purification, and characterization of recombinant proteins.
Detection FLAG-tagged proteins are commonly detected using anti-FLAG antibodies such as M1 and M2 clones in western blotting, ELISA, immunofluorescence, and immunoprecipitation assays.
Purification FLAG affinity resins and magnetic beads enable rapid purification of FLAG-tagged proteins under native or denaturing conditions.
Research relevance The FLAG tag is one of the most widely used epitope tags in molecular biology, biotechnology, and recombinant protein production.

Data

1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2).

FAQ & Publications

Frequently Asked Questions

What applications has the mouse anti-DYDDDDK tag monoclonal antibody (FG4R) been validated for?

This antibody is suitable for Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA applications.

How should the mouse anti-DYDDDDK tag antibody be stored to maintain its stability?

Store the antibody at -20°C for long-term storage up to 2 years or more. After the first thaw, centrifuge to maximize product recovery and aliquot to avoid repeated freeze-thaw cycles. Aliquots can be stored at 4°C for several days to weeks.

What is the recommended dilution range for the antibody in Western blot and ICC/IF assays?

For Western blotting, the recommended dilution is between 1:1000 and 1:3000. For ICC/IF, use a dilution range of 1:500 to 1:2000. For other assays like dot blot, ELISA, or immunoprecipitation, optimal dilutions should be determined empirically by titration.

What is the immunogen used to generate the mouse anti-DYDDDDK tag monoclonal antibody (FG4R)?

The immunogen is a synthetic DYKDDDDK (FLAG) peptide conjugated to keyhole limpet hemocyanin (KLH).

Which secondary antibodies are compatible with this mouse anti-DYDDDDK tag antibody for detection?

Compatible secondaries include goat anti-mouse IgG, H&L chain specific, conjugated to peroxidase, biotin, or FITC. Both standard and cross-absorbed polyclonal antibodies are suitable for use.

Publications

pmid	title	authors	citation
36765089	Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability.	Zaili Luo, Dazhuan Xin, Yunfei Liao, Kalen Berry, Sean Ogurek, Feng Zhang, Liguo Zhang, Chuntao Zhao, Rohit Rao, Xinran Dong, Hao Li, Jianzhong Yu, Yifeng Lin, Guoying Huang, Lingli Xu, Mei Xin, Ryuichi Nishinakamura, Jiyang Yu, Marcel Kool, Stefan M Pfister, Martine F Roussel, Wenhao Zhou, William A Weiss, Paul Andreassen, Q Richard Lu	Nat Commun 14:762
36685538	Duck TRIM29 negatively regulates type I IFN production by targeting MAVS.	Weiqiang Li, Yating Song, Yuqing Du, Zhanhong Huang, Meng Zhang, Zuxian Chen, Zhuoliang He, Yangbao Ding, Junsheng Zhang, Luxiang Zhao, Hailiang Sun, Peirong Jiao	Front Immunol 13:1016214
36622167	Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa.	Adam Kawalek, Krzysztof Glabski, Aneta Agnieszka Bartosik, Dominika Wozniak, Magdalena Kusiak, Jan Gawor, Karolina Zuchniewicz, Grazyna Jagura-Burdzy	Microbiol Spectr 11:e0428922
36441793	GluR2Q and GluR2R AMPA Subunits are not Targets of lypd2 Interaction.	Anna Lauriello, Quinn McVeigh, Rou-Jia Sung	PLoS One 17:e0278278
35906245	TRIM28-dependent SUMOylation protects the adult ovary from activation of the testicular pathway.	Moïra Rossitto, Stephanie Déjardin, Chris M Rands, Stephanie Le Gras, Roberta Migale, Mahmoud-Reza Rafiee, Yasmine Neirijnck, Alain Pruvost, Anvi Laetitia Nguyen, Guillaume Bossis, Florence Cammas, Lionel Le Gallic, Dagmar Wilhelm, Robin Lovell-Badge, Brigitte Boizet-Bonhoure, Serge Nef, Francis Poulat	Nat Commun 13:4412

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.