mouse anti-MBP monoclonal antibody (MBP61R) 6906

Price range: $100.00 through $2,600.00

Antibody summary

Mouse monoclonal to MBP
Suitable for: WB,ICC/IF,ELISA
Reacts with: tagged fusion proteins
Isotype: IgG2b
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 6906parent Categories: antibody, epitope tag Tag: maltose binding protein (MBP)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-MBP monoclonal antibody (MBP61R) 6906

antibody
Database link: P0AEX9 Sequence - MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIK VTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTW DAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEP YFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAE AAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIP QMSAFWYAVRTAVINAASGRQTVDEALKDAQTRITK
Tested applications WB,ICC/IF,ELISA
Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Immunogen Purified recombinant MBP fusion protein expressed in?E. Coli
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions -20°C for 2 years or more. °Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Storage buffer PBS, pH 7.2, 0.05% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG2b - Isotype Control

target relevance
Discosoma sp. RFP Red fluorescent protein drFP583
Protein names Red fluorescent protein drFP583
Protein family Belongs to the GFP family
Function Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts
Structure Homotetramer
Post-translational modification Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), oxidation of Tyr-(N+1) to didehydrotyrosine, and formation of a double bond to the alpha-amino nitrogen of residue Xaa-N. Maturation of the chromophore requires nothing other than molecular oxygen
Keywords 3D-structure, Chromophore, Luminescence, Photoprotein
Sequence MRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVKLKVTKGGPLPFAWDI LSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGCFIY KVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHKALKLKDGGHYLVEFKSI YMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHHLFL
UniProt accession: Q9U6Y8

Data

1:2000 (0.5µg/mL) Ab dilution probed against purified MBP: 50ng (1), 24ng (2), 12ng (3), 6ng (4), and 3ng (5).

FAQ & Publications

Frequently Asked Questions

What applications is the mouse anti-MBP monoclonal antibody (MBP61R) suitable for?

This antibody is suitable for Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA applications.

How should the mouse anti-MBP monoclonal antibody be stored to maintain its stability?

Store the antibody at -20°C for up to 2 years or longer. After the first thaw, centrifuge to maximize product recovery, aliquot to avoid repeated freeze-thaw cycles, and keep aliquots at 4°C if using within days to weeks.

What is the host species and clonality of the MBP61R antibody?

The antibody is a mouse monoclonal antibody of the IgG2b isotype.

What is the recommended dilution range for using this antibody in Western blot and ICC/IF?

For Western blotting, the recommended dilution is between 1:1000 and 1:3000. For ICC/IF, a dilution range of 1:500 to 1:2000 is suggested.

What is the immunogen used to generate this mouse anti-MBP monoclonal antibody?

The immunogen is purified recombinant maltose binding protein (MBP) fusion protein expressed in Escherichia coli.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.