| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Rabbit IgG H&L Chain |
| available sizes | 0.5 mg |
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
$208.00
Antibody summary
- Goat polyclonal to Rabbit IgG, H&L Chain Specific, FITC
- crossabsorbed
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 0.5 mg
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunohistochemistry: use at a dilution of 1:50-1:500 Immunocytochemistry: use at a dilution of 1:50-1:500. Immunofluorescence: use at a dilution of 1:50-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Prep |
| Immunogen Rabbit IgG |
| Size and concentration 0.5mg and 1 mg/mL |
| Form liquid |
| Storage Instructions Store at 2-8°C. Shelf life is one (1) year from date of receipt. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries NA |
| Isotype control NA |
| target relevance |
|---|
| rabbit IgG |
| Protein names Rabbit immunoglobulin G |
| Alternative names Rabbit IgG, Oryctolagus cuniculus IgG, Rabbit gamma immunoglobulin |
| Function Rabbit IgG antibodies bind antigens with high specificity and mediate immune protection through pathogen neutralization, opsonization, complement activation, and antibody-dependent cellular responses. |
| Subcellular location Secreted; present in blood plasma, lymph, extracellular fluids, and tissues. |
| Structure Rabbit IgG is composed of two identical gamma heavy chains and two identical light chains linked by disulfide bonds to form a Y-shaped immunoglobulin molecule containing Fab and Fc regions. |
| Description Rabbit immunoglobulin G (IgG) is the predominant antibody isotype found in rabbit serum and extracellular fluids. It mediates antigen recognition and immune protection through neutralization, opsonization, complement activation, and Fc receptor-mediated effector functions. |
| Related entities relationship: part_of; entity: Immunoglobulin G relationship: related_to; entity: Rabbit IgM relationship: related_to; entity: Rabbit IgA |
| Keywords Antibody, Immunoglobulin, Humoral immunity, Adaptive immunity |
| Structure Rabbit IgG is a tetrameric antibody consisting of two gamma heavy chains and two light chains linked by disulfide bonds. |
| Function Rabbit IgG mediates antigen recognition, pathogen neutralization, opsonization, complement activation, and Fc receptor-dependent immune responses. |
| Distribution Rabbit IgG is the major immunoglobulin isotype found in rabbit serum and extracellular fluids. |
| Applications Rabbit IgG is frequently used as an immunogen for generating anti-rabbit secondary antibodies and as a control antigen in ELISA, western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. |
| Research relevance Rabbit antibodies are widely used in biomedical research because rabbits often generate high-affinity antibodies against epitopes that may be weakly immunogenic in mice. Anti-rabbit IgG secondary antibodies are among the most commonly used detection reagents in life science research. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What species does the goat anti-rabbit IgG FITC conjugated antibody specifically target?
This antibody specifically targets Rabbit IgG heavy and light (H&L) chains.
Which applications has this FITC conjugated goat anti-rabbit IgG antibody been tested for?
It has been validated for use in Western blot (WB), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA.
What are the recommended dilution ranges for immunohistochemistry and immunofluorescence when using this antibody?
For IHC, ICC, and immunofluorescence applications, the suggested dilution range is 1:50 to 1:500, though end users should optimize dilutions for their specific assays.
How should this antibody be stored to maintain its stability and shelf life?
Store the antibody at 2-8°C, and it has a shelf life of one year from the date of receipt. The antibody is supplied in phosphate buffered saline (PBS) with 0.2% BSA and 0.09% sodium azide as a preservative.
What is the concentration and form of the goat anti-rabbit IgG FITC conjugated antibody provided?
The antibody is supplied as a liquid at a concentration of 1 mg/mL, with a total amount of 0.5 mg per vial.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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