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chicken anti-6X His tag polyclonal antibody 8278

$100.00$2,600.00

Antibody summary

  • Chicken polyclonal to 6X His tag
  • Suitable for: ELISA
  • Reacts with: tagged fusion proteins
  • Isotype: IgY
  • 100 µg, 25 µg, 1 mg
SKU: 8278parent Categories: , Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

chicken

isotype

IgY

clonality

polyclonal

concentration

1 mg/mL

applications

ELISA

available sizes

1 mg, 100 µg, 25 µg

Available product – chicken anti-6X His tag polyclonal antibody 8278

antibody
Database link:
6x His tag
Tested applications
ELISA
Recommended dilutions
user optimized
Immunogen
Highly purified HHHHHH conjugated with KLH (6xHIS)
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C
Storage buffer
PBS, pH 7.2, 0.09% NaN3
Purity
affinity purified
Clonality
polyclonal
Isotype
IgY
Compatible secondaries
goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036
goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317
goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718
goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control
Chicken polyclonal - Isotype Control
target relevance
6X His tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify 6X His tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, 6X His tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and 6x His tag
Biotechnology
The 6x Histidine (6x His) tag has become an indispensable tool in both biotechnology and scientific research, particularly in the field of recombinant protein expression and purification. The 6x His tag consists of six consecutive histidine residues genetically fused to a protein of interest. This small peptide sequence offers several advantages, making it highly desirable for the generation of fusion proteins. One of its main benefits is its ability to bind to immobilized metal ions, such as nickel or cobalt, with high affinity. This property allows for efficient and selective purification of the fusion protein through metal affinity chromatography, simplifying the protein purification process and yielding highly pure and concentrated protein samples. Furthermore, the 6His tag also facilitates easy detection and quantification of the fusion protein using anti-His antibodies in techniques like Western blotting. The versatility and simplicity of the 6x His tag have significantly advanced research efforts in protein expression, characterization, and functional analysis, allowing researchers to explore the structure and function of a wide range of proteins in diverse biological systems.

Data

ELISA-image-chicken-anti-6X His tag-polyclonal-antibody-8278

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation
35148180Asymmetric Notch activity by differential inheritance of lysosomes in human neural stem cellsBohl B, Jabali A, Ladewig J, Koch P.Sci Adv. 2022 Feb 11;8(6):eabl5792. doi: 10.1126/sciadv.abl5792. Epub 2022 Feb 11.

Protocols

relevant to this product
ELISA

Documents

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