chicken anti-6X His tag polyclonal antibody 8278

Price range: $100.00 through $2,600.00

Antibody summary

Chicken polyclonal to 6X His tag
Suitable for: ELISA
Reacts with: tagged fusion proteins
Isotype: IgY
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 8278parent Categories: antibody, epitope tag Tag: 6XHis tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	chicken
isotype	IgY
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

chicken anti-6X His tag polyclonal antibody 8278

antibody
Database link: 6x His tag
Tested applications ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with HHHHHH as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen Highly purified HHHHHH conjugated with KLH (6xHIS)
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgY
Compatible secondaries goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036 goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317 goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718 goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control Chicken polyclonal - Isotype Control

target relevance
6X His tag
Protein names Hexahistidine tag
Alternative names 6xHis tag, His tag, Hexa-His tag, Polyhistidine tag, His6 tag, 6His tag
Function The 6×His tag enables purification and detection of recombinant proteins through coordination of histidine residues with immobilized metal ions such as nickel, cobalt, copper, or zinc.
Description The 6×His tag is a synthetic peptide tag consisting of six consecutive histidine residues. It is one of the most widely used affinity tags for recombinant protein purification and detection.
Related entities relationship: binds_to; entity: Nickel ion relationship: binds_to; entity: Ni-NTA relationship: binds_to; entity: Cobalt affinity resin relationship: modified_form_of; entity: 8×His tag relationship: modified_form_of; entity: 10×His tag
Keywords Affinity tag, Protein purification, IMAC, Recombinant protein, Protein detection
Sequence HHHHHH
Structure The 6×His tag consists of six consecutive histidine residues (HHHHHH) and is typically fused to the N- or C-terminus of recombinant proteins.
Function The histidine residues coordinate transition metal ions, enabling affinity purification and detection of tagged proteins.
Purification His-tagged proteins are commonly purified using immobilized metal affinity chromatography (IMAC), including Ni-NTA and cobalt-based resins.
Detection His-tagged proteins can be detected using anti-His antibodies, anti-polyhistidine antibodies, nickel conjugates, or IMAC reagents.
Research relevance The 6×His tag is one of the most widely used recombinant protein tags because of its small size, minimal impact on protein folding, and compatibility with rapid purification workflows.

Data

ELISA-image-chicken-anti-6X His tag-polyclonal-antibody-8278

graphed with benchmark antibodies free graphing tool

FAQ & Publications

Frequently Asked Questions

What applications is the chicken anti-6X His tag polyclonal antibody suitable for?

This antibody is suitable for ELISA, Western blotting, and immunohistochemistry (IHC) applications to detect 6X His tagged fusion proteins.

How should the chicken anti-6X His tag polyclonal antibody be stored to maintain stability?

The antibody should be stored at 2-8°C in its supplied storage buffer, which consists of PBS at pH 7.2 with 0.09% sodium azide.

What is the host species and isotype of this anti-6X His tag antibody?

The antibody is a chicken polyclonal antibody of the IgY isotype.

What is the immunogen used to produce the chicken anti-6X His tag polyclonal antibody?

The immunogen is highly purified 6X Histidine peptide (HHHHHH) conjugated to keyhole limpet hemocyanin (KLH).

Publications

pmid	title	authors	citation
35148180	Asymmetric Notch activity by differential inheritance of lysosomes in human neural stem cells.	Bettina Bohl, Ammar Jabali, Julia Ladewig, Philipp Koch	Sci Adv 8:eabl5792

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.