| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Rabbit IgG H&L Chain |
| available sizes | 1 mg |
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
$208.00
Antibody summary
- Goat polyclonal to Rabbit IgG, H&L Chain Specific-FITC
- Suitable for: IHC,ICC/IF
- Isotype: IgG
- 1 mg
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunohistochemistry: use at a dilution of 1:50-1:500 Immunocytochemistry: use at a dilution of 1:50-1:500. Immunofluorescence: use at a dilution of 1:50-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Prep |
| Immunogen Rabbit IgG |
| Size and concentration 1mg and 1 mg/mL |
| Form liquid |
| Storage Instructions Store at 2-8°C. Shelf life is one (1) year from date of receipt. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries NA |
| Isotype control NA |
| target relevance |
|---|
| rabbit IgG |
| Protein names Rabbit immunoglobulin G |
| Alternative names Rabbit IgG, Oryctolagus cuniculus IgG, Rabbit gamma immunoglobulin |
| Function Rabbit IgG antibodies bind antigens with high specificity and mediate immune protection through pathogen neutralization, opsonization, complement activation, and antibody-dependent cellular responses. |
| Subcellular location Secreted; present in blood plasma, lymph, extracellular fluids, and tissues. |
| Structure Rabbit IgG is composed of two identical gamma heavy chains and two identical light chains linked by disulfide bonds to form a Y-shaped immunoglobulin molecule containing Fab and Fc regions. |
| Description Rabbit immunoglobulin G (IgG) is the predominant antibody isotype found in rabbit serum and extracellular fluids. It mediates antigen recognition and immune protection through neutralization, opsonization, complement activation, and Fc receptor-mediated effector functions. |
| Related entities relationship: part_of; entity: Immunoglobulin G relationship: related_to; entity: Rabbit IgM relationship: related_to; entity: Rabbit IgA |
| Keywords Antibody, Immunoglobulin, Humoral immunity, Adaptive immunity |
| Structure Rabbit IgG is a tetrameric antibody consisting of two gamma heavy chains and two light chains linked by disulfide bonds. |
| Function Rabbit IgG mediates antigen recognition, pathogen neutralization, opsonization, complement activation, and Fc receptor-dependent immune responses. |
| Distribution Rabbit IgG is the major immunoglobulin isotype found in rabbit serum and extracellular fluids. |
| Applications Rabbit IgG is frequently used as an immunogen for generating anti-rabbit secondary antibodies and as a control antigen in ELISA, western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. |
| Research relevance Rabbit antibodies are widely used in biomedical research because rabbits often generate high-affinity antibodies against epitopes that may be weakly immunogenic in mice. Anti-rabbit IgG secondary antibodies are among the most commonly used detection reagents in life science research. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What are the recommended applications and dilutions for the Goat anti-Rabbit IgG H&L chain specific FITC conjugated polyclonal antibody?
This antibody is suitable for Western blot (WB), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA. The recommended dilution range for IHC, ICC, and immunofluorescence is 1:50 to 1:500. End users should determine the optimal dilution for their specific applications.
How should the Goat anti-Rabbit IgG H&L chain specific FITC conjugated polyclonal antibody be stored to maintain stability?
The antibody should be stored at 2-8°C and has a shelf life of one year from the date of receipt. It is provided in a phosphate buffered saline (PBS) storage buffer containing 0.2% BSA and 0.09% sodium azide.
What are the recommended applications and dilutions for using the goat anti-rabbit IgG H&L chain specific FITC conjugated antibody?
This antibody is suitable for immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), Western blot (WB), and ELISA. Recommended dilution ranges are 1:50 to 1:500 for IHC, ICC, and immunofluorescence. Optimal dilutions should be determined by the end user based on their specific application.
How should the goat anti-rabbit IgG H&L chain specific FITC conjugated antibody be stored to maintain stability?
The antibody should be stored at 2-8°C. It is provided in a liquid form with a storage buffer consisting of phosphate buffered saline (PBS) containing 0.2% BSA and 0.09% sodium azide. The shelf life is one year from the date of receipt.
What is the immunogen and specificity of this secondary antibody?
The immunogen used is rabbit IgG. This is a goat polyclonal antibody specific to the heavy and light chains (H&L) of rabbit IgG, conjugated with FITC for fluorescent detection.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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