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Loading control antibodies

Antibodies used as loading controls are essential tools in molecular biology and protein research to ensure accurate and reliable quantification of protein samples. Loading controls are typically constitutively expressed proteins that serve as a reference or internal standard for normalizing protein levels across different experimental conditions or samples. Antibodies against loading control proteins, such as beta-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), or tubulin, are commonly used for this purpose. These antibodies recognize highly abundant and stable proteins that are present in relatively constant amounts in most cells and tissues. By including a loading control antibody in experimental analyses, researchers can account for variations in protein sample loading, gel transfer efficiency, and antibody detection. This allows for accurate normalization of protein expression levels and ensures that any observed changes in target protein expression or abundance are not due to unequal protein loading. Antibodies used as loading controls provide a valuable reference point and increase the reliability and reproducibility of protein quantification experiments.
9052Actin (pan)rabbit polyclonalhuman mouse rat cow pigWBICCIHC
5132beta actinmouse monoclonal (BA3R)human mouse rat rabbit hamsterWBICCELISA
9038beta actinrabbit monoclonalhuman mouse rat rabbit hamsterWBICCELISA
5007beta Tubulinmouse monoclonal (BT7R)human mouse rat rabbit hamsterWBICCELISA
9003beta Tubulinrabbit monoclonalhuman mouse ratWBICCIHC
3427ERK1mouse monoclonal (E19)human mouseWB
9031ERK1rabbit polyclonalhuman mouseWB
1916ERK1/2mouse monoclonal (E31R)humanWB
1937GAPDHmouse monoclonal (GA1R)human mouse rat rabbit hamsterWBICCELISA
9013GAPDHrabbit polyclonalhuman mouse ratWBICCIHC
9023Hsp60rabbit polyclonalhuman mouse ratWBICCIHC