| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Rabbit IgG H&L Chain |
| available sizes | 0.5 mg |
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
$208.00
Antibody summary
- Goat polyclonal to Rabbit IgG, H&L Chain Specific, Biotin
- crossabsorbed
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 1 mg
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunohistochemistry: use at a dilution of 1:50-1:500 Immunocytochemistry: use at a dilution of 1:50-1:500. Immunofluorescence: use at a dilution of 1:50-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Prep |
| Immunogen Rabbit IgG |
| Size and concentration 0.5mg and 1 mg/mL |
| Form liquid |
| Storage Instructions Store at 2-8°C. Shelf life is one (1) year from date of receipt. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries NA |
| Isotype control NA |
| target relevance |
|---|
| rabbit IgG |
| Protein names Rabbit immunoglobulin G |
| Alternative names Rabbit IgG, Oryctolagus cuniculus IgG, Rabbit gamma immunoglobulin |
| Function Rabbit IgG antibodies bind antigens with high specificity and mediate immune protection through pathogen neutralization, opsonization, complement activation, and antibody-dependent cellular responses. |
| Subcellular location Secreted; present in blood plasma, lymph, extracellular fluids, and tissues. |
| Structure Rabbit IgG is composed of two identical gamma heavy chains and two identical light chains linked by disulfide bonds to form a Y-shaped immunoglobulin molecule containing Fab and Fc regions. |
| Description Rabbit immunoglobulin G (IgG) is the predominant antibody isotype found in rabbit serum and extracellular fluids. It mediates antigen recognition and immune protection through neutralization, opsonization, complement activation, and Fc receptor-mediated effector functions. |
| Related entities relationship: part_of; entity: Immunoglobulin G relationship: related_to; entity: Rabbit IgM relationship: related_to; entity: Rabbit IgA |
| Keywords Antibody, Immunoglobulin, Humoral immunity, Adaptive immunity |
| Structure Rabbit IgG is a tetrameric antibody consisting of two gamma heavy chains and two light chains linked by disulfide bonds. |
| Function Rabbit IgG mediates antigen recognition, pathogen neutralization, opsonization, complement activation, and Fc receptor-dependent immune responses. |
| Distribution Rabbit IgG is the major immunoglobulin isotype found in rabbit serum and extracellular fluids. |
| Applications Rabbit IgG is frequently used as an immunogen for generating anti-rabbit secondary antibodies and as a control antigen in ELISA, western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. |
| Research relevance Rabbit antibodies are widely used in biomedical research because rabbits often generate high-affinity antibodies against epitopes that may be weakly immunogenic in mice. Anti-rabbit IgG secondary antibodies are among the most commonly used detection reagents in life science research. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What applications is the goat anti-rabbit IgG H&L chain specific biotin conjugated antibody suitable for?
This antibody is suitable for Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC/IF), and ELISA applications.
How should the goat anti-rabbit IgG biotin conjugated antibody be stored to maintain stability?
Store the antibody at 2-8°C. It has a shelf life of one year from the date of receipt when stored under these conditions.
What is the recommended dilution range for immunohistochemistry and immunofluorescence using this antibody?
The recommended dilution for immunohistochemistry, immunocytochemistry, and immunofluorescence is between 1:50 and 1:500. Users should optimize the dilution for their specific applications.
What is the host species and clonality of this secondary antibody?
The antibody is a polyclonal goat antibody raised against rabbit IgG heavy and light chains.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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