rabbit anti-V5 tag polyclonal antibody 2475

Price range: $100.00 through $2,600.00

Antibody summary

Rabbit polyclonal to V5 tag
Suitable for: ELISA
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 2475parent Categories: antibody, epitope tag Tag: V5

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-V5 tag polyclonal antibody 2475

antibody
Database link: V5 tag sequence -GKPIPNPLLGLDST Derived from V5 95-108. Example strain H3N8 P11208
Tested applications ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with V5 as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen Highly purified V5 peptide conjugated with KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
V5 tag
Protein names V5 epitope tag
Alternative names V5 tag, V5 epitope, SV5 tag, Pk tag
Function The V5 tag is an artificial peptide epitope used for the detection, purification, and characterization of recombinant proteins. It is recognized by anti-V5 antibodies in applications including western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation.
Description The V5 tag is a synthetic peptide epitope derived from the P and V proteins of Human orthorubulavirus 5 (formerly Simian Virus 5, SV5). It is commonly fused to recombinant proteins for detection, purification, and localization studies.
Keywords Epitope tag, Protein detection, Protein purification, Recombinant protein
Sequence GKPIPNPLLGLDST
Structure The V5 tag is a 14-amino acid peptide epitope with the sequence GKPIPNPLLGLDST derived from the P and V proteins of Human orthorubulavirus 5 (formerly SV5).
Function The V5 tag is used as a molecular tag for the detection, purification, and localization of recombinant proteins.
Applications V5-tagged proteins can be detected by western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation.
Research relevance The V5 tag is widely used in recombinant protein expression systems and serves as an alternative to FLAG, HA, Myc, and His tags.

Data

ELISA-image-rabbit-anti-V5 tag-polyclonal-antibody-2475

ELISA analysis with V5 antibody 2475

FAQ & Publications

Frequently Asked Questions

What applications is the rabbit anti-V5 tag polyclonal antibody 2475 validated for?

This antibody has been tested and is suitable for ELISA, blotting, and immunohistochemistry (IHC) applications. Optimal working dilutions should be experimentally determined by the user.

How should the rabbit anti-V5 tag polyclonal antibody 2475 be stored to maintain stability?

The antibody should be stored refrigerated at 2-8°C in its supplied storage buffer, which consists of PBS at pH 7.2 with 0.09% sodium azide as a preservative.

What is the host species and clonality of the anti-V5 tag antibody 2475, and what is its isotype?

The antibody is a rabbit-derived polyclonal antibody of the IgG isotype.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.