chicken anti-HA tag polyclonal antibody 9232

Price range: $100.00 through $2,600.00

Antibody summary

Chicken polyclonal to HA tag
Suitable for: WB, ICC/IF, ELISA
Reacts with: tagged fusion proteins
Isotype: IgY
100 µg, 25 µg, 1mg

available sizes

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SKU: 9232parent Categories: antibody, epitope tag Tag: HA tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	chicken
isotype	IgY
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

chicken anti-HA tag polyclonal antibody 9232

antibody
Database link: Sequence -YPYDVPDYA Derived from HA 118-127. Example strain H3N8 Q03909
Tested applications WB,ICC/IF,ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with YPYDVPDYA as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen YPYDVPDYA (influenza hemagglutinin-HAepitope)
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgY
Compatible secondaries goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036 goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317 goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718 goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control Chicken polyclonal - Isotype Control

target relevance
HA tag
Protein names Hemagglutinin epitope tag
Alternative names HA tag, HA epitope, Influenza HA tag, YPYDVPDYA tag
Function The HA tag is an artificial peptide epitope commonly fused to recombinant proteins to facilitate detection, purification, localization, and immunoprecipitation using anti-HA antibodies.
Description The HA tag is a synthetic peptide epitope derived from the human influenza A virus hemagglutinin (HA) protein. It is widely used for detection, purification, and characterization of recombinant proteins.
Keywords Epitope tag, Protein detection, Protein purification, Immunoprecipitation, Recombinant protein
Sequence YPYDVPDYA
Structure The HA tag is a 9-amino acid peptide epitope with the sequence YPYDVPDYA derived from the influenza A virus hemagglutinin protein.
Function The HA tag is used as a molecular tag for detection, purification, immunoprecipitation, and localization of recombinant proteins.
Detection HA-tagged proteins are commonly detected using anti-HA monoclonal antibodies such as clones 12CA5, 3F10, HA.11, and C29F4.
Applications HA-tagged proteins are routinely analyzed by western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, immunoprecipitation, and affinity purification.
Research relevance The HA tag is one of the most widely used epitope tags in molecular biology and biotechnology and is often used alongside FLAG, Myc, V5, and His tags.

Data

Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed permeabilized, with 0.1% Triton X-100 in CHO cells transfected with GFP-HA constructs labeling with HA tag antibody at 5 µg/mL, followed by Goat Anti-chicken IgG (H&L), Alexa 594 conjugated antibody at 2.5 µg/mL (red). Nucleus was counterstained with DAPI (blue).

FAQ & Publications

Frequently Asked Questions

What applications is the chicken anti-HA tag polyclonal antibody 9232 suitable for?

The chicken anti-HA tag polyclonal antibody 9232 is suitable for Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA applications.

How should the chicken anti-HA tag polyclonal antibody 9232 be stored to maintain stability?

This antibody should be stored at 2-8°C in a storage buffer consisting of PBS at pH 7.2 with 0.09% sodium azide (NaN3) to maintain stability and activity.

Publications

pmid	title	authors	citation
34321103	Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instability.	Sheldon Decombe, François Loll, Laura Caccianini, Kévin Affannoukoué, Ignacio Izeddin, Julien Mozziconacci, Christophe Escudé, Judith Lopes	Epigenetics Chromatin 14:35
34228711	Synaptotagmin-7-mediated activation of spontaneous NMDAR currents is disrupted in bipolar disorder susceptibility variants.	Qiu-Wen Wang, Ying-Han Wang, Bing Wang, Yun Chen, Si-Yao Lu, Jun Yao	PLoS Biol 19:e3001323
33008920	N-Terminal Targeting of Regulator of G Protein Signaling Protein 2 for F-Box Only Protein 44-Mediated Proteasomal Degradation.	Harrison J McNabb, Stephanie Gonzalez, Christine S Muli, Benita Sjögren	Mol Pharmacol 98:677-685
31991108	Treatment of a Mouse Model of ALS by In Vivo Base Editing.	Colin K W Lim, Michael Gapinske, Alexandra K Brooks, Wendy S Woods, Jackson E Powell, M Alejandra Zeballos C, Jackson Winter, Pablo Perez-Pinera, Thomas Gaj	Mol Ther 28:1177-1189
31792216	A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion.	Peter G Boone, Lauren K Rochelle, Joshua D Ginzel, Veronica Lubkov, Wendy L Roberts, P J Nicholls, Cheryl Bock, Mei Lang Flowers, Richard J von Furstenberg, Barry R Stripp, Pankaj Agarwal, Alexander D Borowsky, Robert D Cardiff, Larry S Barak, Marc G Caron, H Kim Lyerly, Joshua C Snyder	Nat Commun 10:5490

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.