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rabbit anti-T7 tag polyclonal antibody 1731

$100.00$2,600.00

Antibody summary

  • Rabbit polyclonal to T7 tag
  • Suitable for: ELISA
  • Reacts with: tagged fusion proteins
  • Isotype: IgG
  • 100 µg, 25 µg, 1 mg
SKU: 1731parent Categories: , Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ELISA

available sizes

1 mg, 100 µg, 25 µg

Available product – rabbit anti-T7 tag polyclonal antibody 1731

antibody
Database link:
T7 tag
Sequence - MASMTGGQQMG
Tested applications
ELISA
Recommended dilutions
user optimized
Immunogen
Highly purified T7 peptide conjugated to KLH
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C
Storage buffer
PBS, pH 7.2, 0.09% NaN3
Purity
affinity purified
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
T7 tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify T7 tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, T7 tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and T7 tag
Biotechnology
The T7 tag has emerged as a valuable tool in scientific research, particularly in molecular biology and protein expression studies. The T7 tag is a short peptide sequence (MASMTGGQQMG) derived from the T7 bacteriophage gene 10, which can be fused to the N or C terminus of a target protein. Its small size and minimal impact on the function of the tagged protein make it an attractive choice as a molecular tag. Researchers frequently use the T7 tag in protein expression systems, such as bacterial, insect, or mammalian cells, to facilitate the identification and purification of the recombinant protein. The T7 tag’s versatility allows for straightforward detection using specific T7 tag antibodies in various experimental assays, including Western blotting and immunoprecipitation. Moreover, the T7 tag can be employed for protein purification through affinity chromatography using T7 tag-specific resins, simplifying downstream characterization and functional studies. The widespread adoption of the T7 tag in research owes to its efficiency, reproducibility, and compatibility with a range of expression systems, making it an indispensable tool for investigating protein interactions, post-translational modifications, and protein localization in diverse biological contexts.

Data

ELISA-image-rabbit-anti-T7 tag-polyclonal-antibody-1731

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
ELISA

Documents

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