rabbit anti-T7 tag polyclonal antibody 1731

Price range: $100.00 through $2,600.00

Antibody summary

Rabbit polyclonal to T7 tag
Suitable for: ELISA
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 1731parent Categories: antibody, epitope tag Tag: T7

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-T7 tag polyclonal antibody 1731

antibody
Database link: T7 tag Sequence - MASMTGGQQMG
Tested applications ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with T7 as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen Highly purified T7 peptide conjugated to KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
T7 tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify T7 tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, T7 tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody. Click for more on: epitope tags and T7 tag
Protein names T7 tag, T7 epitope, T7 promoter (in other contexts)
Biotechnology The T7 tag has emerged as a valuable tool in scientific research, particularly in molecular biology and protein expression studies. The T7 tag is a short peptide sequence (MASMTGGQQMG) derived from the T7 bacteriophage gene 10, which can be fused to the N or C terminus of a target protein. Its small size and minimal impact on the function of the tagged protein make it an attractive choice as a molecular tag. Researchers frequently use the T7 tag in protein expression systems, such as bacterial, insect, or mammalian cells, to facilitate the identification and purification of the recombinant protein. The T7 tag's versatility allows for straightforward detection using specific T7 tag antibodies in various experimental assays, including Western blotting and immunoprecipitation. Moreover, the T7 tag can be employed for protein purification through affinity chromatography using T7 tag-specific resins, simplifying downstream characterization and functional studies. The widespread adoption of the T7 tag in research owes to its efficiency, reproducibility, and compatibility with a range of expression systems, making it an indispensable tool for investigating protein interactions, post-translational modifications, and protein localization in diverse biological contexts.

Data

ELISA-image-rabbit-anti-T7 tag-polyclonal-antibody-1731

FAQ & Publications

Frequently Asked Questions

What applications is the rabbit anti-T7 tag polyclonal antibody 1731 suitable for?

This antibody is suitable for ELISA, blotting, and immunohistochemistry (IHC) applications.

What is the recommended storage condition for this antibody?

The antibody should be stored at 2-8°C in PBS buffer, pH 7.2, containing 0.09% sodium azide.

What is the host species and clonality of this anti-T7 tag antibody?

The antibody is a rabbit polyclonal IgG.

What is the immunogen used to generate this rabbit anti-T7 tag antibody?

The immunogen is a highly purified T7 peptide conjugated to keyhole limpet hemocyanin (KLH).

Which secondary antibodies are compatible with the rabbit anti-T7 tag polyclonal antibody 1731?

Compatible secondary antibodies include goat anti-rabbit IgG H&L chain specific conjugates such as peroxidase, biotin, FITC, and cross-absorbed variants.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.