rabbit anti-T7 tag polyclonal antibody 1731

Price range: $100.00 through $2,600.00

Antibody summary

Rabbit polyclonal to T7 tag
Suitable for: ELISA
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 1731parent Categories: antibody, epitope tag Tag: T7

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-T7 tag polyclonal antibody 1731

antibody
Database link: T7 tag Sequence - MASMTGGQQMG
Tested applications ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with T7 as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen Highly purified T7 peptide conjugated to KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
T7 tag
Protein names T7 epitope tag
Alternative names T7 tag, T7 epitope, Gene 10 tag, MASMTGGQQMG tag
Function The T7 tag is an artificial peptide epitope used for detection and characterization of recombinant proteins using anti-T7 tag antibodies.
Description The T7 tag is a peptide epitope derived from the major capsid protein (gene 10 protein) of bacteriophage T7. It is commonly used as a recombinant protein tag for detection, purification, and characterization of fusion proteins.
Keywords Epitope tag, Protein detection, Protein purification, Immunoprecipitation, Recombinant protein
Sequence MASMTGGQQMG
Structure The T7 tag is an 11-amino acid peptide epitope with the sequence MASMTGGQQMG derived from the bacteriophage T7 gene 10 major capsid protein.
Function The T7 tag is used as a molecular epitope tag for detection, purification, and characterization of recombinant proteins.
Detection T7-tagged proteins can be detected using anti-T7 tag antibodies in western blotting, ELISA, immunofluorescence, immunocytochemistry, and immunoprecipitation assays.
Applications The T7 tag is commonly incorporated into expression vectors and fused to recombinant proteins to facilitate detection and purification.
Research relevance The T7 tag is widely used in bacterial expression systems and molecular biology workflows and serves as an alternative to FLAG, HA, Myc, V5, and His tags.

Data

ELISA-image-rabbit-anti-T7 tag-polyclonal-antibody-1731

FAQ & Publications

Frequently Asked Questions

What applications is the rabbit anti-T7 tag polyclonal antibody 1731 suitable for?

This antibody is suitable for ELISA, blotting, and immunohistochemistry (IHC) applications.

What is the recommended storage condition for this antibody?

The antibody should be stored at 2-8°C in PBS buffer, pH 7.2, containing 0.09% sodium azide.

What is the host species and clonality of this anti-T7 tag antibody?

The antibody is a rabbit polyclonal IgG.

What is the immunogen used to generate this rabbit anti-T7 tag antibody?

The immunogen is a highly purified T7 peptide conjugated to keyhole limpet hemocyanin (KLH).

Which secondary antibodies are compatible with the rabbit anti-T7 tag polyclonal antibody 1731?

Compatible secondary antibodies include goat anti-rabbit IgG H&L chain specific conjugates such as peroxidase, biotin, FITC, and cross-absorbed variants.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.