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rabbit anti-mCherry polyclonal antibody 2999

$100.00$2,600.00

Antibody summary

  • Rabbit polyclonal to mCherry
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: tagged fusion proteins
  • Isotype: IgG
  • 100 µg, 25 µg, 1 mg
SKU: 2999parent Categories: , Tags: ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

available sizes

1 mg, 100 µg, 25 µg

Available product – rabbit anti-mCherry polyclonal antibody 2999

antibody
Database link:
mCherry X5DSL3
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:1000 IF/IHC: 1:500
Immunogen
Full length recombinant mCherry protein expressed in and purified from E. coli
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
affinity purified
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
Protein expression of mCherry and mCherry tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, mCherry fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody.

Click for more on: epitope tags and mCherry
Biotechnology
mCherry, a widely used fluorescent protein, has become an indispensable tool in various areas of scientific research. Derived from the red fluorescent protein DsRed, mCherry emits bright red fluorescence when excited with green light, making it an excellent choice for fluorescence microscopy and live-cell imaging studies. Researchers commonly utilize mCherry as a reporter gene, allowing them to track and visualize specific proteins, organelles, or cellular structures in real-time within living cells and organisms. Its compatibility with other fluorescent proteins enables dual-labeling experiments and multiplexing, providing valuable insights into dynamic cellular processes and interactions. Additionally, mCherry’s stability and resistance to photobleaching enhance its utility for long-term imaging studies. Moreover, mCherry has been employed in various fields, including cell biology, molecular biology, neuroscience, and developmental biology, where its fluorescence serves as a powerful tool to investigate cellular behavior, protein localization, and gene expression patterns. As research methodologies continue to advance, mCherry’s versatility and reliability continue to contribute significantly to our understanding of complex biological phenomena and pave the way for new discoveries in the life sciences.

Data

ICC/IF-image-rabbit-anti-mCherry-polyclonal-antibody-2999
Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA, construct, in red, and stained with rabbit pAb to mCherry, 2999, dilution 1:1,000, in green. The blue is Hoechst staining of nuclear DNA. 2999 antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells do not react with the antibody, as a result only their nuclei are visible.
IHC-image-rabbit-anti-mCherry-polyclonal-antibody-2999
This antibody was made against a recombinant construct expressed in and purified from E. coli. The sequence is identical to that found in a series of widely used expression vectors and is identical to Uniprot entry D1MPT3.1 MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK51 LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER101 VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA151 SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV201 NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYKChromogenic immunostaining of formalin fixed paraffin embedded oxytocin-Tdtomato Cre mouse brain section with rabbit pAb to mCherry, 2999, dilution 1:5,000, detected with DAB (brown) using the Vector Elite 8718C-HRP detection and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. 2999 specifically detected the soma and axons of Cre activated neurons expressing Tdtomato. Our antibody recognizes tdTomato since it is very similar in primary sequence to mCherry. Mouse select image at left for larger view.
WB-image-rabbit-anti-mCherry-polyclonal-antibody-2999
Western blot analysis of HEK293 cell lysates using rabbit pAb to mCherry, 2999, dilution 1:3,000, in green, and mouse mAb to GAPDH, 5157 dilution 1:2,000, in red: [1] protein standard, [2] HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [4] HEK293 cells transfected with pCI-mod vector expressing one mCherry-HA protein domain, and [5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain. The 2999 antibody recognizes tdTomato and mCherry proteins revealing major bands at about 60kDa and 30kDa, in green, respectfully, but does not recognize GFP. The red band at 37kDa corresponds to GAPDH protein here used as a loading control.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

There are 6 publications in our database for this antibody or clone. Here are the latest 5, for more click below.


pmidtitleauthorscitation
37503287Chronic striatal cholinergic interneuron excitation induces clinically-relevant dystonic behavior in miceGemperli K, Lu X, Chintalapati K, Rust A, Bajpai R, Suh N, Blackburn J, Gelineau-Morel R, Kruer MC, Mingbundersuk D, O'Malley J, Tochen L, Waugh J, Wu S, Feyma T, Perlmutter J, Mennerick S, McCall J, Aravamuthan BR.bioRxiv [Preprint]. 2023 Jul 21:2023.07.19.549778. doi: 10.1101/2023.07.19.549778.
31636119Nucleoplasmin is a limiting component in the scaling of nuclear size with cytoplasmic volumeChen P, Tomschik M, Nelson KM, Oakey J, Gatlin JC, Levy DL.J Cell Biol. 2019 Dec 2;218(12):4063-4078. doi: 10.1083/jcb.201902124. Epub 2019 Oct 21.
15558047Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent proteinShaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY.Nat Biotechnol. 2004 Dec;22(12):1567-72. doi: 10.1038/nbt1037. Epub 2004 Nov 21.
11050231Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine)Heikal AA, Hess ST, Baird GS, Tsien RY, Webb WW.Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11996-2001. doi: 10.1073/pnas.97.22.11996.
11050230The structure of the chromophore within DsRed, a red fluorescent protein from coralGross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY.Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11990-5. doi: 10.1073/pnas.97.22.11990.

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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