ICC/IF image chicken anti HA tag polyclonal antibody 9232

rabbit anti-HA tag polyclonal antibody 6395

Price range: $100.00 through $2,600.00

Antibody summary

Rabbit polyclonal to HA tag
Suitable for: WB, ICC/IF, ELISA
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 6395parent Categories: antibody, epitope tag Tag: HA tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-HA tag polyclonal antibody 6395

antibody
Database link: Sequence -YPYDVPDYA Derived from HA 118-127. Example strain H3N8 Q03909
Tested applications WB,ICC/IF,ELISA
Recommended dilutions user optimized
Application Notes Rabbits were immunized with highly purified YPYDVPDYA (influenza hemagglutinin-HA-epitope) and the resulting antiserum was collected. Antibody was immunoaffinity purified off an antigen containing immunosorbent. Antibody concentration was determined using an absorbance at 280 nm: 1.4 equals 1.0 mg of IgG.
Immunogen Highly purified YPYDVPDYA (influenza hemagglutinin-HA-epitope)
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
HA tag
Protein names Hemagglutinin epitope tag
Alternative names HA tag, HA epitope, Influenza HA tag, YPYDVPDYA tag
Function The HA tag is an artificial peptide epitope commonly fused to recombinant proteins to facilitate detection, purification, localization, and immunoprecipitation using anti-HA antibodies.
Description The HA tag is a synthetic peptide epitope derived from the human influenza A virus hemagglutinin (HA) protein. It is widely used for detection, purification, and characterization of recombinant proteins.
Keywords Epitope tag, Protein detection, Protein purification, Immunoprecipitation, Recombinant protein
Sequence YPYDVPDYA
Structure The HA tag is a 9-amino acid peptide epitope with the sequence YPYDVPDYA derived from the influenza A virus hemagglutinin protein.
Function The HA tag is used as a molecular tag for detection, purification, immunoprecipitation, and localization of recombinant proteins.
Detection HA-tagged proteins are commonly detected using anti-HA monoclonal antibodies such as clones 12CA5, 3F10, HA.11, and C29F4.
Applications HA-tagged proteins are routinely analyzed by western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, immunoprecipitation, and affinity purification.
Research relevance The HA tag is one of the most widely used epitope tags in molecular biology and biotechnology and is often used alongside FLAG, Myc, V5, and His tags.

Data

Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed permabilied, with 0.1% Triton X-100 in CHO cells transfected with GFP-HA constructs labeling with HA antibody at 5 µg/mL, followed by Donkey Anti-rabbit IgG (H&L), Alexa 594 conjugated antibody at 2.5 µg/mL (red). Nucleus was counterstained with DAPI (blue).

Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed permabilied, with 0.1% Triton X-100 in CHO cells labeling with HA tag antibody at 5 µg/mL, followed by Donkey Anti-rabbit IgG (H&L), Alexa 594 conjugated antibody at 2.5 µg/mL (red). Nucleus was counterstained with DAPI (blue).

FAQ & Publications

Frequently Asked Questions

What applications is the rabbit anti-HA tag polyclonal antibody 6395 validated for?

This antibody has been tested and validated for use in Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), and enzyme-linked immunosorbent assay (ELISA).

What is the immunogen used to generate the rabbit anti-HA tag polyclonal antibody 6395?

The antibody was produced by immunizing rabbits with the highly purified peptide YPYDVPDYA, which corresponds to the influenza hemagglutinin HA epitope (HA 118-127).

What is the recommended storage condition and buffer for this HA tag antibody?

The antibody should be stored at 2-8°C in phosphate-buffered saline (PBS) at pH 7.2 containing 0.09% sodium azide (NaN3) as a preservative.

Is the rabbit anti-HA tag polyclonal antibody 6395 suitable for detecting HA-tagged fusion proteins from different species?

Yes, this antibody specifically recognizes the HA epitope tag sequence YPYDVPDYA on tagged fusion proteins, independent of the species origin of the fusion protein.

What is the concentration and form of the rabbit anti-HA tag polyclonal antibody 6395 supplied?

The antibody is supplied as a liquid at a concentration of 1 mg/mL, available in sizes of 25 µg, 100 µg, and 1 mg.

Publications

pmid	title	authors	citation
35357490	Polymerase theta-helicase promotes end joining by stripping single-stranded DNA-binding proteins and bridging DNA ends.	Jeffrey M Schaub, Michael M Soniat, Ilya J Finkelstein	Nucleic Acids Res 50:3911-3921
34585037	Identification and Functional Characterization of a Novel Androgen Receptor Coregulator, EAP1.	Atsushi Yokoyama, Takumi Kouketsu, Yuri Otsubo, Erika Noro, Shun Sawatsubashi, Hiroki Shima, Ikuro Satoh, Sadafumi Kawamura, Takashi Suzuki, Kazuhiko Igarashi, Akira Sugawara	J Endocr Soc 5:bvab150
32719507	Epigenetic cell fate in Candida albicans is controlled by transcription factor condensates acting at super-enhancer-like elements.	Corey Frazer, Mae I Staples, Yoori Kim, Matthew Hirakawa, Maureen A Dowell, Nicole V Johnson, Aaron D Hernday, Veronica H Ryan, Nicolas L Fawzi, Ilya J Finkelstein, Richard J Bennett	Nat Microbiol 5:1374-1389
32341095	An Epilepsy-Associated SV2A Mutation Disrupts Synaptotagmin-1 Expression and Activity-Dependent Trafficking.	Callista B Harper, Christopher Small, Elizabeth C Davenport, Darryl W Low, Karen J Smillie, Ramón Martínez-Mármol, Frédéric A Meunier, Michael A Cousin	J Neurosci 40:4586-4595
31153714	RPA Phosphorylation Inhibits DNA Resection.	Michael M Soniat, Logan R Myler, Hung-Che Kuo, Tanya T Paull, Ilya J Finkelstein	Mol Cell 75:145-153.e5

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.