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rabbit anti-HA tag polyclonal antibody 6395

$100.00$2,600.00

Antibody summary

  • Rabbit polyclonal to HA tag
  • Suitable for: WB, ICC/IF, ELISA
  • Reacts with: tagged fusion proteins
  • Isotype: IgG
  • 100 µg, 25 µg, 1 mg
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SKU: 6395parent Categories: , Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ELISA, ICC/IF, WB

available sizes

1 mg, 100 µg, 25 µg

antibody
Database link:
Sequence -YPYDVPDYA
Derived from HA 118-127. Example strain H3N8 Q03909
Tested applications
WB,ICC/IF,ELISA
Recommended dilutions
user optimized
Immunogen
Highly purified YPYDVPDYA (influenza hemagglutinin-HA-epitope)
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C
Storage buffer
PBS, pH 7.2, 0.09% NaN3
Purity
affinity purified
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit polyclonal - Isotype Control
target relevance
HA tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify HA tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, HA tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and HA tag
Biotechnology
The HA tag, derived from the human influenza hemagglutinin protein, is a widely employed tool in recombinant protein expression and has become an invaluable asset in molecular biology research. When incorporated at the N- or C-terminus of a target protein, the HA tag serves as a short, immunogenic peptide sequence, enabling efficient detection, purification, and characterization of the expressed protein. Its small size minimizes disruption to the target protein’s structure and function, making it suitable for a diverse range of applications. The HA tag is specifically recognized by commercially available high-affinity antibodies, facilitating the precise and sensitive detection of the tagged protein in techniques like Western blotting and immunofluorescence. Additionally, the HA tag is compatible with affinity chromatography, enabling straightforward and effective purification of the tagged protein from complex cellular extracts. This versatility has made the HA tag a valuable tool for studying protein interactions, localization, and post-translational modifications, providing researchers with valuable insights into cellular processes and molecular mechanisms across various fields of biology and biotechnology.

Data

ICC/IF-image-rabbit-anti-HA tag-polyclonal-antibody-6395
ICC/IF-image-rabbit-anti-HA tag-polyclonal-antibody-6395

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation
35357490Polymerase theta-helicase promotes end joining by stripping single-stranded DNA-binding proteins and bridging DNA endsSchaub JM, Soniat MM, Finkelstein IJ.Nucleic Acids Res. 2022 Apr 22;50(7):3911-3921. doi: 10.1093/nar/gkac119.
34585037Identification and Functional Characterization of a Novel Androgen Receptor Coregulator, EAP1Yokoyama A, Kouketsu T, Otsubo Y, Noro E, Sawatsubashi S, Shima H, Satoh I, Kawamura S, Suzuki T, Igarashi K, Sugawara A.J Endocr Soc. 2021 Sep 13;5(11):bvab150. doi: 10.1210/jendso/bvab150. eCollection 2021 Nov 1.
32719507Epigenetic cell fate in Candida albicans is controlled by transcription factor condensates acting at super-enhancer-like elementsFrazer C, Staples MI, Kim Y, Hirakawa M, Dowell MA, Johnson NV, Hernday AD, Ryan VH, Fawzi NL, Finkelstein IJ, Bennett RJ.Nat Microbiol. 2020 Nov;5(11):1374-1389. doi: 10.1038/s41564-020-0760-7. Epub 2020 Jul 27.
32341095An Epilepsy-Associated SV2A Mutation Disrupts Synaptotagmin-1 Expression and Activity-Dependent TraffickingHarper CB, Small C, Davenport EC, Low DW, Smillie KJ, Martínez-Mármol R, Meunier FA, Cousin MA.J Neurosci. 2020 Jun 3;40(23):4586-4595. doi: 10.1523/JNEUROSCI.0210-20.2020. Epub 2020 Apr 27.
31153714RPA Phosphorylation Inhibits DNA ResectionSoniat MM, Myler LR, Kuo HC, Paull TT, Finkelstein IJ.Mol Cell. 2019 Jul 11;75(1):145-153.e5. doi: 10.1016/j.molcel.2019.05.005. Epub 2019 May 29.
30541127Intrinsically disordered regions regulate both catalytic and non-catalytic activities of the MutLα mismatch repair complexKim Y, Furman CM, Manhart CM, Alani E, Finkelstein IJ.Nucleic Acids Res. 2019 Feb 28;47(4):1823-1835. doi: 10.1093/nar/gky1244.
30487222Phage Mu Gam protein promotes NHEJ in concert with Escherichia coli ligaseBhattacharyya S, Soniat MM, Walker D, Jang S, Finkelstein IJ, Harshey RM.Proc Natl Acad Sci U S A. 2018 Dec 11;115(50):E11614-E11622. doi: 10.1073/pnas.1816606115. Epub 2018 Nov 28.
30343903Assembly and Translocation of a CRISPR-Cas Primed Acquisition ComplexDillard KE, Brown MW, Johnson NV, Xiao Y, Dolan A, Hernandez E, Dahlhauser SD, Kim Y, Myler LR, Anslyn EV, Ke A, Finkelstein IJ.Cell. 2018 Nov 1;175(4):934-946.e15. doi: 10.1016/j.cell.2018.09.039. Epub 2018 Oct 18.
29760102Broad receptor engagement of an emerging global coronavirus may potentiate its diverse cross-species transmissibilityLi W, Hulswit RJG, Kenney SP, Widjaja I, Jung K, Alhamo MA, van Dieren B, van Kuppeveld FJM, Saif LJ, Bosch BJ.Proc Natl Acad Sci U S A. 2018 May 29;115(22):E5135-E5143. doi: 10.1073/pnas.1802879115. Epub 2018 May 14.
29738496Endogenous Purification of NR4A2 (Nurr1) Identified Poly(ADP-Ribose) Polymerase 1 as a Prime Coregulator in Human Adrenocortical H295R CellsNoro E, Yokoyama A, Kobayashi M, Shimada H, Suzuki S, Hosokawa M, Takehara T, Parvin R, Shima H, Igarashi K, Sugawara A.Int J Mol Sci. 2018 May 8;19(5):1406. doi: 10.3390/ijms19051406.
28867292Single-Molecule Imaging Reveals How Mre11-Rad50-Nbs1 Initiates DNA Break RepairMyler LR, Gallardo IF, Soniat MM, Deshpande RA, Gonzalez XB, Kim Y, Paull TT, Finkelstein IJ.Mol Cell. 2017 Sep 7;67(5):891-898.e4. doi: 10.1016/j.molcel.2017.08.002. Epub 2017 Aug 31.
28666121Massively Parallel Biophysical Analysis of CRISPR-Cas Complexes on Next Generation Sequencing ChipsJung C, Hawkins JA, Jones SK Jr, Xiao Y, Rybarski JR, Dillard KE, Hussmann J, Saifuddin FA, Savran CA, Ellington AD, Ke A, Press WH, Finkelstein IJ.Cell. 2017 Jun 29;170(1):35-47.e13. doi: 10.1016/j.cell.2017.05.044.
28062036Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of DNA Mismatch RepairBrown MW, de la Torre A, Finkelstein IJ.Methods Enzymol. 2017;582:221-238. doi: 10.1016/bs.mie.2016.08.006. Epub 2016 Oct 24.
27484226Gtpbp2 is a positive regulator of Wnt signaling and maintains low levels of the Wnt negative regulator AxinGillis WQ, Kirmizitas A, Iwasaki Y, Ki DH, Wyrick JM, Thomsen GH.Cell Commun Signal. 2016 Aug 2;14(1):15. doi: 10.1186/s12964-016-0138-x.
26837705Dynamic DNA binding licenses a repair factor to bypass roadblocks in search of DNA lesionsBrown MW, Kim Y, Williams GM, Huck JD, Surtees JA, Finkelstein IJ.Nat Commun. 2016 Feb 3;7:10607. doi: 10.1038/ncomms10607.
24747194Assaying proteasomal degradation in a cell-free system in plantsGarcía-Cano E, Zaltsman A, Citovsky V.J Vis Exp. 2014 Mar 26;(85):51293. doi: 10.3791/51293.

Protocols

relevant to this product
Western blot
ICC

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