ICC/IF image chicken anti HA tag polyclonal antibody 9232

rabbit anti-HA tag polyclonal antibody 6395

Price range: $100.00 through $2,600.00

Antibody summary

Rabbit polyclonal to HA tag
Suitable for: WB, ICC/IF, ELISA
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 6395parent Categories: antibody, epitope tag Tag: HA tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-HA tag polyclonal antibody 6395

antibody
Database link: Sequence -YPYDVPDYA Derived from HA 118-127. Example strain H3N8 Q03909
Tested applications WB,ICC/IF,ELISA
Recommended dilutions user optimized
Application Notes Rabbits were immunized with highly purified YPYDVPDYA (influenza hemagglutinin-HA-epitope) and the resulting antiserum was collected. Antibody was immunoaffinity purified off an antigen containing immunosorbent. Antibody concentration was determined using an absorbance at 280 nm: 1.4 equals 1.0 mg of IgG.
Immunogen Highly purified YPYDVPDYA (influenza hemagglutinin-HA-epitope)
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
HA tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify HA tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, HA tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody. Click for more on: epitope tags and HA tag
Protein names HA tag, Hemagglutinin tag, YPYDVPDYA
Biotechnology The HA tag, derived from the human influenza hemagglutinin protein, is a widely employed tool in recombinant protein expression and has become an invaluable asset in molecular biology research. When incorporated at the N- or C-terminus of a target protein, the HA tag serves as a short, immunogenic peptide sequence, enabling efficient detection, purification, and characterization of the expressed protein. Its small size minimizes disruption to the target protein's structure and function, making it suitable for a diverse range of applications. The HA tag is specifically recognized by commercially available high-affinity antibodies, facilitating the precise and sensitive detection of the tagged protein in techniques like Western blotting and immunofluorescence. Additionally, the HA tag is compatible with affinity chromatography, enabling straightforward and effective purification of the tagged protein from complex cellular extracts. This versatility has made the HA tag a valuable tool for studying protein interactions, localization, and post-translational modifications, providing researchers with valuable insights into cellular processes and molecular mechanisms across various fields of biology and biotechnology.

Data

Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed permabilied, with 0.1% Triton X-100 in CHO cells transfected with GFP-HA constructs labeling with HA antibody at 5 µg/mL, followed by Donkey Anti-rabbit IgG (H&L), Alexa 594 conjugated antibody at 2.5 µg/mL (red). Nucleus was counterstained with DAPI (blue).

Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed permabilied, with 0.1% Triton X-100 in CHO cells labeling with HA tag antibody at 5 µg/mL, followed by Donkey Anti-rabbit IgG (H&L), Alexa 594 conjugated antibody at 2.5 µg/mL (red). Nucleus was counterstained with DAPI (blue).

FAQ & Publications

Frequently Asked Questions

What applications is the rabbit anti-HA tag polyclonal antibody 6395 validated for?

This antibody has been tested and validated for use in Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), and enzyme-linked immunosorbent assay (ELISA).

What is the immunogen used to generate the rabbit anti-HA tag polyclonal antibody 6395?

The antibody was produced by immunizing rabbits with the highly purified peptide YPYDVPDYA, which corresponds to the influenza hemagglutinin HA epitope (HA 118-127).

What is the recommended storage condition and buffer for this HA tag antibody?

The antibody should be stored at 2-8°C in phosphate-buffered saline (PBS) at pH 7.2 containing 0.09% sodium azide (NaN3) as a preservative.

Is the rabbit anti-HA tag polyclonal antibody 6395 suitable for detecting HA-tagged fusion proteins from different species?

Yes, this antibody specifically recognizes the HA epitope tag sequence YPYDVPDYA on tagged fusion proteins, independent of the species origin of the fusion protein.

What is the concentration and form of the rabbit anti-HA tag polyclonal antibody 6395 supplied?

The antibody is supplied as a liquid at a concentration of 1 mg/mL, available in sizes of 25 µg, 100 µg, and 1 mg.

Publications

pmid	title	authors	citation
35357490	Polymerase theta-helicase promotes end joining by stripping single-stranded DNA-binding proteins and bridging DNA ends.	Jeffrey M Schaub, Michael M Soniat, Ilya J Finkelstein	Nucleic Acids Res 50:3911-3921
34585037	Identification and Functional Characterization of a Novel Androgen Receptor Coregulator, EAP1.	Atsushi Yokoyama, Takumi Kouketsu, Yuri Otsubo, Erika Noro, Shun Sawatsubashi, Hiroki Shima, Ikuro Satoh, Sadafumi Kawamura, Takashi Suzuki, Kazuhiko Igarashi, Akira Sugawara	J Endocr Soc 5:bvab150
32719507	Epigenetic cell fate in Candida albicans is controlled by transcription factor condensates acting at super-enhancer-like elements.	Corey Frazer, Mae I Staples, Yoori Kim, Matthew Hirakawa, Maureen A Dowell, Nicole V Johnson, Aaron D Hernday, Veronica H Ryan, Nicolas L Fawzi, Ilya J Finkelstein, Richard J Bennett	Nat Microbiol 5:1374-1389
32341095	An Epilepsy-Associated SV2A Mutation Disrupts Synaptotagmin-1 Expression and Activity-Dependent Trafficking.	Callista B Harper, Christopher Small, Elizabeth C Davenport, Darryl W Low, Karen J Smillie, Ramón Martínez-Mármol, Frédéric A Meunier, Michael A Cousin	J Neurosci 40:4586-4595
31153714	RPA Phosphorylation Inhibits DNA Resection.	Michael M Soniat, Logan R Myler, Hung-Che Kuo, Tanya T Paull, Ilya J Finkelstein	Mol Cell 75:145-153.e5

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.