| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 1 mg, 100 µg, 25 µg |
rabbit anti-HA tag polyclonal antibody 6395
$100.00 – $2,600.00
Antibody summary
- Rabbit polyclonal to HA tag
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG
- 100 µg, 25 µg, 1 mg
rabbit anti-HA tag polyclonal antibody 6395
| target relevance |
|---|
| HA tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify HA tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, HA tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody. Click for more on: epitope tags and HA tag |
| Biotechnology The HA tag, derived from the human influenza hemagglutinin protein, is a widely employed tool in recombinant protein expression and has become an invaluable asset in molecular biology research. When incorporated at the N- or C-terminus of a target protein, the HA tag serves as a short, immunogenic peptide sequence, enabling efficient detection, purification, and characterization of the expressed protein. Its small size minimizes disruption to the target protein???s structure and function, making it suitable for a diverse range of applications. The HA tag is specifically recognized by commercially available high-affinity antibodies, facilitating the precise and sensitive detection of the tagged protein in techniques like Western blotting and immunofluorescence. Additionally, the HA tag is compatible with affinity chromatography, enabling straightforward and effective purification of the tagged protein from complex cellular extracts. This versatility has made the HA tag a valuable tool for studying protein interactions, localization, and post-translational modifications, providing researchers with valuable insights into cellular processes and molecular mechanisms across various fields of biology and biotechnology. |
Data
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Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 16 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 35357490 | Polymerase theta-helicase promotes end joining by stripping single-stranded DNA-binding proteins and bridging DNA ends | Schaub JM, Soniat MM, Finkelstein IJ. | Nucleic Acids Res. 2022 Apr 22;50(7):3911-3921. doi: 10.1093/nar/gkac119. |
| 34585037 | Identification and Functional Characterization of a Novel Androgen Receptor Coregulator, EAP1 | Yokoyama A, Kouketsu T, Otsubo Y, Noro E, Sawatsubashi S, Shima H, Satoh I, Kawamura S, Suzuki T, Igarashi K, Sugawara A. | J Endocr Soc. 2021 Sep 13;5(11):bvab150. doi: 10.1210/jendso/bvab150. eCollection 2021 Nov 1. |
| 32719507 | Epigenetic cell fate in Candida albicans is controlled by transcription factor condensates acting at super-enhancer-like elements | Frazer C, Staples MI, Kim Y, Hirakawa M, Dowell MA, Johnson NV, Hernday AD, Ryan VH, Fawzi NL, Finkelstein IJ, Bennett RJ. | Nat Microbiol. 2020 Nov;5(11):1374-1389. doi: 10.1038/s41564-020-0760-7. Epub 2020 Jul 27. |
| 32341095 | An Epilepsy-Associated SV2A Mutation Disrupts Synaptotagmin-1 Expression and Activity-Dependent Trafficking | Harper CB, Small C, Davenport EC, Low DW, Smillie KJ, Martínez-Mármol R, Meunier FA, Cousin MA. | J Neurosci. 2020 Jun 3;40(23):4586-4595. doi: 10.1523/JNEUROSCI.0210-20.2020. Epub 2020 Apr 27. |
| 31153714 | RPA Phosphorylation Inhibits DNA Resection | Soniat MM, Myler LR, Kuo HC, Paull TT, Finkelstein IJ. | Mol Cell. 2019 Jul 11;75(1):145-153.e5. doi: 10.1016/j.molcel.2019.05.005. Epub 2019 May 29. |
Protocols
| relevant to this product |
|---|
| Western blot ICC |
Documents
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