ICC/IF image rabbit anti GFP polyclonal antibody 1290

rabbit anti-GFP polyclonal antibody 1290

$100.00 – $2,600.00

Antibody summary

Rabbit polyclonal to GFP
Suitable for: WB, ICC/IF
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

SKU: 1290parent Categories: antibody, epitope tag Tag: GFP

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-GFP polyclonal antibody 1290

antibody
Database link: P42212
Tested applications WB,ICC/IF
Recommended dilutions WB: 1:1000-5000 IF/ICC 1:2000-5000
Immunogen Recombinant AcGFP expressed in and purified from E. coli
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein expression of GFP and GFP tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, GFP fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody. Click for more on: epitope tags and GFP
Protein names Green fluorescent protein
Gene names GFP,GFP
Protein family GFP family
Mass 26886Da
Function Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Tissues Photocytes.
Structure Monomer.
Post-translational modification Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnology Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Green fluorescent protein can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when Thr-203 is replaced by Tyr). Further generation of mutants led to more stable proteins (at 37 degrees Celsius for example) with brighter fluorescence and longer fluorescence lifetimes. Fluorescent proteins and their mutated allelic forms have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions (PubMed:17685514, PubMed:17685554, PubMed:8578587, PubMed:8707053, PubMed:9145105, PubMed:9154981, PubMed:9759496, PubMed:9782051). Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy (PubMed:17685514).
Target Relevance information above includes information from UniProt accession: P42212
The UniProt Consortium

Immunofluorescent analysis of transfected HEK293 cells with a GFP construct, in green, and stained with rabbit pAb to GFP, 1290, dilution 1:2,000 in red. The blue is Hoechst staining of nuclear DNA. The 1290 antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear orange-yellow in color.

IHC-image-rabbit-anti-GFP-polyclonal-antibody-290

Chromogenic immunostaining of formalin fixed paraffin embedded section of GFP injected mouse brain with rabbit pAb to GFP, 1290, dilution 1:2,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. 1290 specifically detected GFP at the injection site.

Western blot analysis of lysates of transfected HEK293 using rabbit pAb to GFP, 1290 in green, dilution 1:2,000. [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct, and [4] cells transfected with mCherry construct. Strong band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct, the antibody does not recognize mCherry. The same blot was simultaneously probed with mouse mAb to beta-tubulin, 1222, dilution 1:10,000, in red. The single band ~50kDa represents beta-tubulin protein expressed in all preparations.

Publications

pmid	title	authors	citation
36323247	The Rb/E2F axis is a key regulator of the molecular signatures instructing the quiescent and activated adult neural stem cell state.	Bensun C Fong, Imane Chakroun, Mohamed Ariff Iqbal, Smitha Paul, Joseph Bastasic, Daniel O'Neil, Edward Yakubovich, Anthony T Bejjani, Nastaran Ahmadi, Anthony Carter, Alysen Clark, Gustavo Leone, David S Park, Noël Ghanem, Renaud Vandenbosch, Ruth S Slack	Cell Rep 41:111578
30888667	Histological Assessment of Cre-loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreER(T2)/Rosa26YFP Mice.	Saad Omais, Nour N Halaby, Karl John Habashy, Carine Jaafar, Anthony T Bejjani, Noël Ghanem	Methods Mol Biol 2045:187-199
13911999	Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea.	O SHIMOMURA, F H JOHNSON, Y SAIGA	J Cell Comp Physiol 59:223-39
12693991	A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants.	Nadya G Gurskaya, Arkady F Fradkov, Natalia I Pounkova, Dmitry B Staroverov, Maria E Bulina, Yurii G Yanushevich, Yulii A Labas, Sergey Lukyanov, Konstantin A Lukyanov	Biochem J 373:403-8
11988576	Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells.	David A Zacharias, Jonathan D Violin, Alexandra C Newton, Roger Y Tsien	Science 296:913-6

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.