rabbit anti-DYDDDDK tag polyclonal antibody 4849

Price range: $100.00 through $2,600.00

Antibody summary

Rabbit polyclonal to DYDDDDK tag
Suitable for: WB, ICC/IF, ELISA
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 4849parent Categories: antibody, epitope tag Tag: DYDDDDK tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-DYDDDDK tag polyclonal antibody 4849

antibody
Database link: DYDDDDK tag (FLAG tag)
Tested applications WB,ICC/IF,ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with DYKDDDDK as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen Highly purified DYKDDDDK epitope conjugated to KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
DDDDK
Protein names FLAG tag
Alternative names FLAG tag, FLAG epitope, DYKDDDDK tag, DYDDDDK tag, FLAG peptide
Function The FLAG tag serves as an affinity and detection tag that can be fused to recombinant proteins. It is recognized by anti-FLAG antibodies and affinity reagents for purification, immunoprecipitation, western blotting, immunofluorescence, flow cytometry, and ELISA.
Description The FLAG tag is a short artificial peptide epitope widely used in recombinant protein expression, purification, detection, and localization studies. The canonical FLAG sequence is DYKDDDDK.
Biological role Recombinant protein detection Affinity purification Immunoprecipitation Protein localization studies
Research relevance FLAG-tagged proteins are extensively used in molecular biology and protein expression systems because the tag is small, highly immunogenic, and generally does not interfere with protein folding or function.
Keywords Epitope tag, Protein purification, Protein detection, Recombinant protein
Structure The FLAG tag is a synthetic peptide epitope with the canonical amino acid sequence DYKDDDDK.
Function FLAG serves as a molecular tag for detection, purification, and characterization of recombinant proteins.
Detection FLAG-tagged proteins are commonly detected using anti-FLAG antibodies such as M1 and M2 clones in western blotting, ELISA, immunofluorescence, and immunoprecipitation assays.
Purification FLAG affinity resins and magnetic beads enable rapid purification of FLAG-tagged proteins under native or denaturing conditions.
Research relevance The FLAG tag is one of the most widely used epitope tags in molecular biology, biotechnology, and recombinant protein production.

Data

Immunofluorescence cell staining- rabbit anti-DYDDDDK tag polyclonal antibody 4849 Immunofluorescent analysis of paraformaldehyde (PFA) fixed, permeabilized with 0.1% triton X-100 CHO cells transfected with GFP-3XDYKDDDK construct (CHO-GFP-2xFLAG) labeling DYKDDDDK tag with antibody #4849 at 5µg/mL, followed by Donkey Anti-rabbit IgG, Alexa 594 conjugated with 2.5µg/mL (red). Nucleus was counterstained with DAPI (blue).

FAQ & Publications

Frequently Asked Questions

What applications is the rabbit anti-DYDDDDK tag polyclonal antibody suitable for?

This antibody is suitable for Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA applications.

How should the rabbit anti-DYDDDDK tag polyclonal antibody be stored to maintain its stability?

The antibody should be stored at 2-8°C in a PBS buffer at pH 7.2 containing 0.09% sodium azide (NaN3).

What is the immunogen used to generate the rabbit anti-DYDDDDK tag polyclonal antibody?

The immunogen is a highly purified DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH).

Publications

pmid	title	authors	citation
35190665	Intersectional, anterograde transsynaptic targeting of neurons receiving monosynaptic inputs from two upstream regions.	Takuma Kitanishi, Mariko Tashiro, Naomi Kitanishi, Kenji Mizuseki	Commun Biol 5:149
22234250	The interaction of the von Hippel-Lindau tumor suppressor and heterochromatin protein 1.	Yanlai Lai, Meihua Song, Kevin Hakala, Susan T Weintraub, Yuzuru Shiio	Arch Biochem Biophys 518:103-10

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.