rabbit anti-DYDDDDK tag polyclonal antibody 4849

$100.00 – $2,600.00

Antibody summary

Rabbit polyclonal to DYDDDDK tag
Suitable for: WB, ICC/IF, ELISA
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

SKU: 4849parent Categories: antibody, epitope tag Tag: DYDDDDK tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-DYDDDDK tag polyclonal antibody 4849

antibody
Database link: DYDDDDK tag (FLAG tag)
Tested applications WB,ICC/IF,ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with DYKDDDDK as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen Highly purified DYKDDDDK epitope conjugated to KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance

DYDDDDK (Flag) tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify DYDDDDK (Flag) tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, DYDDDDK (Flag) tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and DYDDDDK (Flag) tag

Biotechnology
The DYDDDDK tag, also known as the FLAG tag, is a short peptide sequence that has become a popular and powerful tool in recombinant protein expression. When fused to the N- or C-terminus of a target protein, the DYDDDDK tag allows for easy detection, purification, and immunoprecipitation of the expressed protein. Its small size and minimal impact on protein folding and function make it an attractive choice for various research applications. The DYDDDDK tag is recognized by commercially available high-affinity antibodies, facilitating the sensitive and specific detection of the tagged protein using techniques like Western blotting or immunofluorescence. Furthermore, the FLAG tag is amenable to purification via affinity chromatography, where anti-FLAG antibodies are immobilized on a resin, enabling efficient and rapid purification of the tagged protein from complex biological samples. The versatility and efficiency of the DYDDDDK tag have revolutionized the field of molecular biology, aiding researchers in studying protein-protein interactions, subcellular localization, and protein dynamics, ultimately advancing our understanding of cellular processes and driving innovative discoveries in various scientific disciplines.

Immunofluorescence cell staining- rabbit anti-DYDDDDK tag polyclonal antibody 4849 Immunofluorescent analysis of paraformaldehyde (PFA) fixed, permeabilized with 0.1% triton X-100 CHO cells transfected with GFP-3XDYKDDDK construct (CHO-GFP-2xFLAG) labeling DYKDDDDK tag with antibody #4849 at 5µg/mL, followed by Donkey Anti-rabbit IgG, Alexa 594 conjugated with 2.5µg/mL (red). Nucleus was counterstained with DAPI (blue).

Publications

pmid	title	authors	citation
35190665	Intersectional, anterograde transsynaptic targeting of neurons receiving monosynaptic inputs from two upstream regions.	Takuma Kitanishi, Mariko Tashiro, Naomi Kitanishi, Kenji Mizuseki	Commun Biol 5:149
22234250	The interaction of the von Hippel-Lindau tumor suppressor and heterochromatin protein 1.	Yanlai Lai, Meihua Song, Kevin Hakala, Susan T Weintraub, Yuzuru Shiio	Arch Biochem Biophys 518:103-10

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.