| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA |
| reactivity | tagged fusion proteins |
| available sizes | 1 mg, 100 µg, 25 µg |
rabbit anti-6X His tag polyclonal antibody 7094
Price range: $100.00 through $2,600.00
Antibody summary
- Rabbit polyclonal to 6X His tag
- Suitable for: ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG
- 100 µg, 25 µg, 1 mg
rabbit anti-6X His tag polyclonal antibody 7094
| antibody |
|---|
| Database link: 6x His tag sequence HHHHHH |
| Tested applications ELISA |
| Recommended dilutions user optimized |
| Application Notes This antibody reacts with HHHHHH as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator. |
| Immunogen Highly purified HHHHHH conjugated with KLH (6xHIS) |
| Size and concentration 25, 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions 2-8°C |
| Storage buffer PBS, pH 7.2, 0.09% NaN3 |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Rabbit polyclonal - Isotype Control |
| target relevance |
|---|
| 6X His tag |
| Protein names Hexahistidine tag |
| Alternative names 6xHis tag, His tag, Hexa-His tag, Polyhistidine tag, His6 tag, 6His tag |
| Function The 6×His tag enables purification and detection of recombinant proteins through coordination of histidine residues with immobilized metal ions such as nickel, cobalt, copper, or zinc. |
| Description The 6×His tag is a synthetic peptide tag consisting of six consecutive histidine residues. It is one of the most widely used affinity tags for recombinant protein purification and detection. |
| Related entities relationship: binds_to; entity: Nickel ion relationship: binds_to; entity: Ni-NTA relationship: binds_to; entity: Cobalt affinity resin relationship: modified_form_of; entity: 8×His tag relationship: modified_form_of; entity: 10×His tag |
| Keywords Affinity tag, Protein purification, IMAC, Recombinant protein, Protein detection |
| Sequence HHHHHH |
| Structure The 6×His tag consists of six consecutive histidine residues (HHHHHH) and is typically fused to the N- or C-terminus of recombinant proteins. |
| Function The histidine residues coordinate transition metal ions, enabling affinity purification and detection of tagged proteins. |
| Purification His-tagged proteins are commonly purified using immobilized metal affinity chromatography (IMAC), including Ni-NTA and cobalt-based resins. |
| Detection His-tagged proteins can be detected using anti-His antibodies, anti-polyhistidine antibodies, nickel conjugates, or IMAC reagents. |
| Research relevance The 6×His tag is one of the most widely used recombinant protein tags because of its small size, minimal impact on protein folding, and compatibility with rapid purification workflows. |
Data
 |
| rabbit anti-6X His tag polyclonal antibody 7094 1:1000 (1µg/mL) and donkey anti-rabbit IgG (H&L) alexa fluor 594 |
 |
| Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilized with 0.1% triton X-100 in CHO cells transfected with 12 tags construct (CHO-12 tags) labeling 6xHIS with rabbit-anti-6x-his tag-polyclonal antibody 7094 1/1000 dilution (1µg/mL), followed by donkey anti-rabbit IgG (H&L), Alexa fluor 594 antibody at 1/500 dilution (4µg/mL) (red). Nucleus was counterstained with DAPI (blue). Bottom panel shows CHO parental cell line staining. |
FAQ & Publications
Frequently Asked Questions
What applications is the rabbit anti-6X His tag polyclonal antibody suitable for?
This antibody is suitable for ELISA, Western blotting, immunohistochemistry (IHC), and immunocytochemistry/immunofluorescence applications, enabling detection and quantification of 6X His tagged recombinant proteins.
How should the rabbit anti-6X His tag polyclonal antibody be stored to maintain stability?
The antibody should be stored refrigerated at 2-8°C in its provided storage buffer, which is PBS at pH 7.2 containing 0.09% sodium azide (NaN3), to ensure optimal stability and activity.
What is the immunogen used to generate this rabbit anti-6X His tag antibody and what is its clonality?
The immunogen is a highly purified 6X histidine peptide (HHHHHH) conjugated with keyhole limpet hemocyanin (KLH). The antibody is polyclonal and of the IgG isotype.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| 36339259 | Activity-based probes trap early active intermediates during metacaspase activation. | Vida Štrancar, Katarina P van Midden, Daniel Krahn, Kyoko Morimoto, Marko Novinec, Christiane Funk, Simon Stael, Christopher J Schofield, Marina Klemenčič, Renier A L van der Hoorn | iScience 25:105247 |
| 35986286 | Modulation of macrophage inflammatory function through selective inhibition of the epigenetic reader protein SP140. | Mohammed Ghiboub, Jan Koster, Peter D Craggs, Andrew Y F Li Yim, Anthony Shillings, Sue Hutchinson, Ryan P Bingham, Kelly Gatfield, Ishtu L Hageman, Gang Yao, Heather P O'Keefe, Aaron Coffin, Amish Patel, Lisa A Sloan, Darren J Mitchell, Thomas G Hayhow, Laurent Lunven, Robert J Watson, Christopher E Blunt, Lee A Harrison, Gordon Bruton, Umesh Kumar, Natalie Hamer, John R Spaull, Danny A Zwijnenburg, Olaf Welting, Theodorus B M Hakvoort, Anje A Te Velde, Johan van Limbergen, Peter Henneman, Rab K Prinjha, Menno P J de Winther, Nicola R Harker, David F Tough, Wouter J de Jonge | BMC Biol 20:182 |
| 35903773 | Human SLC4A11 does not complement BOR1 or support borate transport in Saccharomyces cerevisiae. | Jean L Beltran, Richara K Bain, Marie J Stiefel, Alexis S McDonnell, Natalie N Carr, Bryan H Thurtle-Schmidt | MicroPubl Biol 2022:N/A |
| 35584627 | Broad and ultra-potent cross-clade neutralization of HIV-1 by a vaccine-induced CD4 binding site bovine antibody. | Behnaz Heydarchi, Danielle S Fong, Hongmei Gao, Natalia A Salazar-Quiroz, Jack M Edwards, Christopher A Gonelli, Samantha Grimley, Turgut E Aktepe, Charlene Mackenzie, William J Wales, Marit J van Gils, Albert Cupo, Isabelle Rouiller, Paul R Gooley, John P Moore, Rogier W Sanders, David Montefiori, Ashish Sethi, Damian F J Purcell | Cell Rep Med 3:100635 |
| 35443160 | A dual-function phage regulator controls the response of cohabiting phage elements via regulation of the bacterial SOS response. | Gil Azulay, Anna Pasechnek, Olga Stadnyuk, Shai Ran-Sapir, Ana Mejia Fleisacher, Ilya Borovok, Nadejda Sigal, Anat A Herskovits | Cell Rep 39:110723 |
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| ELISA |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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