mouse anti-V5 tag monoclonal antibody (V5.E10) 7678

Price range: $100.00 through $2,600.00

Antibody summary

Mouse monoclonal to V5 tag
Suitable for: WB,ICC/IF,ELISA
Reacts with: tagged fusion proteins
Isotype: IgG1
100 µg, 25 µg, 1 mg

available sizes

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SKU: 7678parent Categories: antibody, epitope tag Tag: V5

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-V5 tag monoclonal antibody (V5.E10) 7678

antibody
Database link: V5 tag Sequence - GKPIPNPLLGLDST
Tested applications WB,ICC/IF,ELISA
Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Immunogen GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Storage buffer PBS, pH 7.2, 0.05% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
V5 tag
Protein names V5 epitope tag
Alternative names V5 tag, V5 epitope, SV5 tag, Pk tag
Function The V5 tag is an artificial peptide epitope used for the detection, purification, and characterization of recombinant proteins. It is recognized by anti-V5 antibodies in applications including western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation.
Description The V5 tag is a synthetic peptide epitope derived from the P and V proteins of Human orthorubulavirus 5 (formerly Simian Virus 5, SV5). It is commonly fused to recombinant proteins for detection, purification, and localization studies.
Keywords Epitope tag, Protein detection, Protein purification, Recombinant protein
Sequence GKPIPNPLLGLDST
Structure The V5 tag is a 14-amino acid peptide epitope with the sequence GKPIPNPLLGLDST derived from the P and V proteins of Human orthorubulavirus 5 (formerly SV5).
Function The V5 tag is used as a molecular tag for the detection, purification, and localization of recombinant proteins.
Applications V5-tagged proteins can be detected by western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation.
Research relevance The V5 tag is widely used in recombinant protein expression systems and serves as an alternative to FLAG, HA, Myc, and His tags.

Data

1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2).

FAQ & Publications

Frequently Asked Questions

What applications is the mouse anti-V5 tag monoclonal antibody (V5.E10) validated for?

This antibody is validated for use in Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA assays.

How should the mouse anti-V5 tag monoclonal antibody (V5.E10) be stored to maintain stability?

Store the antibody at -20°C for long-term stability of 2 years or more. After the first thaw, centrifuge to maximize product recovery, aliquot to avoid repeated freeze-thaw cycles, and keep aliquots at 4°C for several days to weeks.

What is the host species and clonality of the anti-V5 tag antibody (V5.E10)?

The antibody is a mouse monoclonal of the IgG1 isotype.

What is the immunogen used to generate the mouse anti-V5 tag monoclonal antibody (V5.E10)?

The immunogen is a synthetic peptide corresponding to the V5 tag sequence GKPIPNPLLGLDST conjugated to KLH.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.