| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Mouse IgG H&L Chain |
| available sizes | 1 mg |
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
$231.00
Antibody summary
- Goat polyclonal to Mouse IgG H&L Chain, Peroxidase
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 1 mg
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunohistochemistry: use at a dilution of 1:50-1:500 Immunocytochemistry: use at a dilution of 1:50-1:500. Immunofluorescence: use at a dilution of 1:50-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Prep |
| Immunogen Mouse IgG |
| Size and concentration 1mg and 1 mg/mL |
| Form liquid |
| Storage Instructions Store at 2-8°C. Shelf life is one (1) year from date of receipt. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.05% Proclin 400 |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries NA |
| Isotype control NA |
| target relevance |
|---|
| mouse IgG |
| Protein names Mouse immunoglobulin G |
| Alternative names Mouse IgG, Mus musculus IgG, Mouse gamma immunoglobulin |
| Function Mouse IgG antibodies specifically recognize antigens and mediate immune protection through neutralization, opsonization, complement activation, antibody-dependent cellular cytotoxicity, and immune complex formation. |
| Subcellular location Secreted; present in blood plasma, lymph, extracellular fluids, and tissues. |
| Structure Mouse IgG is composed of two identical gamma heavy chains and two identical light chains linked by disulfide bonds, forming a Y-shaped immunoglobulin molecule containing Fab and Fc regions. |
| Description Mouse immunoglobulin G (IgG) is the predominant antibody isotype found in mouse serum and extracellular fluids. It plays a central role in humoral immunity through antigen recognition, pathogen neutralization, complement activation, and Fc receptor-mediated effector functions. |
| Related entities relationship: has_subclass; entity: Mouse IgG1 relationship: has_subclass; entity: Mouse IgG2a relationship: has_subclass; entity: Mouse IgG2b relationship: has_subclass; entity: Mouse IgG3 relationship: part_of; entity: Immunoglobulin G |
| Keywords Antibody, Immunoglobulin, Humoral immunity, Adaptive immunity |
| Structure Mouse IgG is a tetrameric antibody consisting of two gamma heavy chains and two light chains linked by disulfide bonds. |
| Function Mouse IgG mediates antigen recognition, pathogen neutralization, opsonization, complement activation, and Fc receptor-dependent immune responses. |
| Subclasses Mouse IgG is divided into four subclasses: IgG1, IgG2a, IgG2b, and IgG3, each exhibiting distinct biological and effector properties. |
| Distribution Mouse IgG is the major immunoglobulin isotype found in mouse serum and extracellular fluids. |
| Research relevance Mouse IgG is extensively used in monoclonal antibody production, immunology research, ELISA, western blotting, immunohistochemistry, flow cytometry, and therapeutic antibody development. Anti-mouse IgG secondary antibodies are among the most commonly used detection reagents in biomedical research. |
Data
 |
| Detection of human p53 by immunoperoxidase. Samples: FFPE serial sections of human stomach carcinoma. Primary Antibody: mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: goat anti-mouse IgG-heavy and light chain Antibody HRP Conjugated (5486 Lot 33) used at a dilution of 1:200 (5µg /ml). Detection: DAB |
FAQ & Publications
Frequently Asked Questions
What applications is the goat anti-mouse IgG H&L chain peroxidase conjugated antibody suitable for?
This polyclonal antibody is suitable for Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry/Immunofluorescence (ICC/IF), and ELISA applications.
How should this antibody be stored to maintain its stability?
The antibody should be stored refrigerated at 2-8°C. It has a shelf life of one year from the date of receipt when stored under these conditions.
What is the recommended dilution range for immunohistochemistry using this goat anti-mouse IgG antibody?
For immunohistochemistry, a dilution range of 1:50 to 1:500 is recommended. However, end users should determine the optimal dilution based on their specific experimental conditions.
What is the host species and clonality of this peroxidase-conjugated secondary antibody?
The antibody is a goat-derived polyclonal antibody, targeting mouse IgG heavy and light chains.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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