| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG2b |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| reactivity | tagged fusion proteins |
| available sizes | 1 mg, 100 µg, 25 µg |
mouse anti-6X His tag monoclonal antibody (HIS.H8) 2966
Price range: $100.00 through $2,600.00
Antibody summary
- Mouse monoclonal to 6X His tag
- Suitable for: WB,ICC/IF,ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG2b
- 100 µg, 25 µg, 1 mg
mouse anti-6X His tag monoclonal antibody (HIS.H8) 2966
| antibody |
|---|
| Database link: 6x His tag sequence HHHHHH |
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test. |
| Immunogen Highly purified HHHHHH conjugated with KLH (6xHIS) |
| Size and concentration 25, 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions -20°C for 2 years or more. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. |
| Storage buffer PBS, pH 7.2, 0.05% NaN3 |
| Purity affinity purified |
| Clonality monoclonal |
| Isotype IgG2b |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG2b - Isotype Control |
| target relevance |
|---|
| 6X His tag |
| Protein names Hexahistidine tag |
| Alternative names 6xHis tag, His tag, Hexa-His tag, Polyhistidine tag, His6 tag, 6His tag |
| Function The 6×His tag enables purification and detection of recombinant proteins through coordination of histidine residues with immobilized metal ions such as nickel, cobalt, copper, or zinc. |
| Description The 6×His tag is a synthetic peptide tag consisting of six consecutive histidine residues. It is one of the most widely used affinity tags for recombinant protein purification and detection. |
| Related entities relationship: binds_to; entity: Nickel ion relationship: binds_to; entity: Ni-NTA relationship: binds_to; entity: Cobalt affinity resin relationship: modified_form_of; entity: 8×His tag relationship: modified_form_of; entity: 10×His tag |
| Keywords Affinity tag, Protein purification, IMAC, Recombinant protein, Protein detection |
| Sequence HHHHHH |
| Structure The 6×His tag consists of six consecutive histidine residues (HHHHHH) and is typically fused to the N- or C-terminus of recombinant proteins. |
| Function The histidine residues coordinate transition metal ions, enabling affinity purification and detection of tagged proteins. |
| Purification His-tagged proteins are commonly purified using immobilized metal affinity chromatography (IMAC), including Ni-NTA and cobalt-based resins. |
| Detection His-tagged proteins can be detected using anti-His antibodies, anti-polyhistidine antibodies, nickel conjugates, or IMAC reagents. |
| Research relevance The 6×His tag is one of the most widely used recombinant protein tags because of its small size, minimal impact on protein folding, and compatibility with rapid purification workflows. |
Data
 |
| LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue). |
 |
| Comparison between anti-His tag (HIS.H8) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2,000 (0.5µg/mL). |
 |
| Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2). |
FAQ & Publications
Frequently Asked Questions
What applications has the mouse anti-6X His tag monoclonal antibody (HIS.H8) been validated for?
This antibody has been tested and is suitable for Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA applications.
How should the mouse anti-6X His tag monoclonal antibody (HIS.H8) be stored to maintain its stability?
The antibody should be stored at -20°C for up to 2 years or longer. After the first thaw, it is recommended to centrifuge to maximize product recovery, aliquot to avoid repeated freeze-thaw cycles, and store aliquots at 4°C for several days to weeks.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| 36640338 | Structural analysis of receptor engagement and antigenic drift within the BA.2 spike protein. | James W Saville, Dhiraj Mannar, Xing Zhu, Alison M Berezuk, Spencer Cholak, Katharine S Tuttle, Faezeh Vahdatihassani, Sriram Subramaniam | Cell Rep 42:111964 |
| 36627706 | Development of SARS-CoV-2 specific IgG and IgA antibodies in serum and milk with different SARS-COV-2 vaccines in lactating women. | Carolina Lechosa-Muñiz, María Paz-Zulueta, Juan Irure-Ventura, Jose Manuel Mendez-Legaza, Rocío Cuesta González, Inés Gómez-Acebo, Marcos López-Hoyos, Javier Llorca, María Jesús Cabero-Pérez | Int Breastfeed J 18:3 |
| 36535926 | Stabilization of MCL-1 by E3 ligase TRAF4 confers radioresistance. | Ming Li, Feng Gao, Xiaoying Li, Yu Gan, Shuangze Han, Xinfang Yu, Haidan Liu, Wei Li | Cell Death Dis 13:1053 |
| 36504569 | Evaluation of PLGA-Encapsulated Recombinant GroEL of S. typhi immune Responses Against Enterohaemorrhagic and Enteropathogenic Escherichia coli. | Milad Parvane, Shahram Nazarian, Emad Kordbache, Javad Fathi, Mohamad Ebrahim Minae, Mohammad Reza Ramezani | Avicenna J Med Biotechnol 14:294-302 |
| 36494335 | SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-β signaling. | Scott B Biering, Francielle Tramontini Gomes de Sousa, Laurentia V Tjang, Felix Pahmeier, Chi Zhu, Richard Ruan, Sophie F Blanc, Trishna S Patel, Caroline M Worthington, Dustin R Glasner, Bryan Castillo-Rojas, Venice Servellita, Nicholas T N Lo, Marcus P Wong, Colin M Warnes, Daniel R Sandoval, Thomas Mandel Clausen, Yale A Santos, Douglas M Fox, Victoria Ortega, Anders M Näär, Ralph S Baric, Sarah A Stanley, Hector C Aguilar, Jeffrey D Esko, Charles Y Chiu, John E Pak, P Robert Beatty, Eva Harris | Nat Commun 13:7630 |
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| 1024 | QC Certificate | Safety Data Sheet |
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