| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Mouse IgG H&L Chain |
| available sizes | 0.5 mg |
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
$208.00
Antibody summary
- Goat polyclonal to Mouse IgG, H&L Chain Specific, Biotin
- crossabsorbed
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 0.5 mg
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunohistochemistry: use at a dilution of 1:50-1:500 Immunocytochemistry: use at a dilution of 1:50-1:500. Immunofluorescence: use at a dilution of 1:50-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Prep |
| Immunogen Mouse IgG |
| Size and concentration 0.5mg and 1 mg/mL |
| Form liquid |
| Storage Instructions Store at 2-8°C. Shelf life is one (1) year from date of receipt. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries NA |
| Isotype control NA |
| target relevance |
|---|
| mouse IgG |
| Protein names Mouse immunoglobulin G |
| Alternative names Mouse IgG, Mus musculus IgG, Mouse gamma immunoglobulin |
| Function Mouse IgG antibodies specifically recognize antigens and mediate immune protection through neutralization, opsonization, complement activation, antibody-dependent cellular cytotoxicity, and immune complex formation. |
| Subcellular location Secreted; present in blood plasma, lymph, extracellular fluids, and tissues. |
| Structure Mouse IgG is composed of two identical gamma heavy chains and two identical light chains linked by disulfide bonds, forming a Y-shaped immunoglobulin molecule containing Fab and Fc regions. |
| Description Mouse immunoglobulin G (IgG) is the predominant antibody isotype found in mouse serum and extracellular fluids. It plays a central role in humoral immunity through antigen recognition, pathogen neutralization, complement activation, and Fc receptor-mediated effector functions. |
| Related entities relationship: has_subclass; entity: Mouse IgG1 relationship: has_subclass; entity: Mouse IgG2a relationship: has_subclass; entity: Mouse IgG2b relationship: has_subclass; entity: Mouse IgG3 relationship: part_of; entity: Immunoglobulin G |
| Keywords Antibody, Immunoglobulin, Humoral immunity, Adaptive immunity |
| Structure Mouse IgG is a tetrameric antibody consisting of two gamma heavy chains and two light chains linked by disulfide bonds. |
| Function Mouse IgG mediates antigen recognition, pathogen neutralization, opsonization, complement activation, and Fc receptor-dependent immune responses. |
| Subclasses Mouse IgG is divided into four subclasses: IgG1, IgG2a, IgG2b, and IgG3, each exhibiting distinct biological and effector properties. |
| Distribution Mouse IgG is the major immunoglobulin isotype found in mouse serum and extracellular fluids. |
| Research relevance Mouse IgG is extensively used in monoclonal antibody production, immunology research, ELISA, western blotting, immunohistochemistry, flow cytometry, and therapeutic antibody development. Anti-mouse IgG secondary antibodies are among the most commonly used detection reagents in biomedical research. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What are the recommended applications and dilutions for the goat anti-mouse IgG H&L chain specific biotin conjugated antibody?
This antibody is suitable for Western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC/IF), and ELISA. Recommended dilution ranges for IHC, ICC, and immunofluorescence are 1:50 to 1:500. Optimal dilution should be determined by the end user for their specific application.
How should I store the goat anti-mouse IgG H&L chain specific biotin conjugated antibody to maintain its stability?
Store the antibody at 2-8°C. The shelf life is one year from the date of receipt. It is supplied in phosphate-buffered saline containing 0.2% BSA and 0.09% sodium azide as a preservative.
What is the host species and clonality of this biotin conjugated secondary antibody?
The antibody is a goat polyclonal antibody, affinity purified, targeting mouse IgG heavy and light chains.
What is the immunogen used to generate this goat anti-mouse IgG antibody?
The immunogen used is mouse IgG, ensuring specificity to mouse immunoglobulin G heavy and light chains.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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