mouse anti-V5 tag monoclonal antibody (V5.E10) 7678

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to V5 tag
Suitable for: WB,ICC/IF,ELISA
Reacts with: tagged fusion proteins
Isotype: IgG1
100 µg, 25 µg, 1 mg

SKU: 7678parent Categories: antibody, epitope tag Tag: V5

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-V5 tag monoclonal antibody (V5.E10) 7678

antibody
Database link: V5 tag Sequence - GKPIPNPLLGLDST
Tested applications WB,ICC/IF,ELISA
Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Immunogen GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage buffer PBS, pH 7.2, 0.05% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance

V5 tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify V5tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, V5 tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and V5 tag

Biotechnology
The V5 tag has become a valuable tool in scientific research, particularly in studies involving the expression and detection of recombinant proteins. The V5 tag is a short peptide sequence (GKPIPNPLLGLDST) that can be fused to the N or C terminus of a target protein, allowing for easy detection and purification. Researchers commonly utilize the V5 tag as an epitope for specific V5 tag antibodies, enabling convenient and sensitive visualization of the tagged protein in various experimental assays, including Western blotting and immunofluorescence. The small size of the V5 tag minimizes potential interference with the function and properties of the tagged protein, making it an attractive choice as a molecular tag. Additionally, the V5 tag can be used for protein purification using affinity chromatography, facilitating the isolation and characterization of the tagged protein. The widespread use of the V5 tag in research owes to its efficiency, reliability, and compatibility with various expression systems, making it an essential tool for investigating protein dynamics, interactions, and cellular localization in diverse biological contexts.

Data

1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2).

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.