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mouse anti-mCherry monoclonal antibody (1C51) 8371

(15 customer reviews)

$100.00$2,600.00

Antibody summary

  • Mouse monoclonal to mCherry
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: tagged fusion proteins
  • Isotype: IgG2a
  • 100 µg, 25 µg, 1 mg
SKU: 8371parent Categories: , Tags: ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG2a

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

available sizes

1 mg, 100 µg, 25 µg

Available product – mouse anti-mCherry monoclonal antibody (1C51) 8371

antibody
Database link:
mCherry X5DSL3
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:2000 IF/IHC: 1:500
Immunogen
Full length recombinant protein
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
affinity purified
Clonality
monoclonal
Isotype
IgG2a
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG2a - Isotype Control
target relevance
Protein expression of mCherry and mCherry tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, mCherry fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody.

Click for more on: epitope tags and mCherry
Biotechnology
mCherry, a widely used fluorescent protein, has become an indispensable tool in various areas of scientific research. Derived from the red fluorescent protein DsRed, mCherry emits bright red fluorescence when excited with green light, making it an excellent choice for fluorescence microscopy and live-cell imaging studies. Researchers commonly utilize mCherry as a reporter gene, allowing them to track and visualize specific proteins, organelles, or cellular structures in real-time within living cells and organisms. Its compatibility with other fluorescent proteins enables dual-labeling experiments and multiplexing, providing valuable insights into dynamic cellular processes and interactions. Additionally, mCherry’s stability and resistance to photobleaching enhance its utility for long-term imaging studies. Moreover, mCherry has been employed in various fields, including cell biology, molecular biology, neuroscience, and developmental biology, where its fluorescence serves as a powerful tool to investigate cellular behavior, protein localization, and gene expression patterns. As research methodologies continue to advance, mCherry’s versatility and reliability continue to contribute significantly to our understanding of complex biological phenomena and pave the way for new discoveries in the life sciences.

Data

ICC/IF-image-mouse-anti-mCherry-monoclonal-antibody-1C51-8371
Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA, construct, in red, and stained with mouse mAb to mCherry, 8371, dilution 1:500, in green. The blue is Hoechst staining of nuclear DNA. 8371 antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells do not react with the antibody, as a result only their nuclei are visible.
IHC-image-mouse-anti-mCherry-monoclonal-antibody-1C51-8371
Chromogenic immunostaining of formalin fixed paraffin embedded mCherry transfected monkey brain with mouse mAb to mCherry, 8371, dilution 1:2,000, detected in DAB (brown) following the ImmPress method. Hematoxylin (blue) was used as the counterstain. 8371 specifically detected the soma and axons of mCherry positive neurons in the cortex, as expected for this model. Mouse select image above for larger view.The sequence of the recombinant mCherry construct used to make this antibody is shown below;1 MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK 51 LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER 101 VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA 151 SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV 201 NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYKThe recombinant construct was expressed in and purified from E. coli. The sequence is identical to that found in a series of widely used expression vectors.
WB-image-mouse-anti-mCherry-monoclonal-antibody-1C51-8371
Western blot analysis of HEK293 cell lysates using mouse mAb to mCherry, 8371, dilution 1:2,000, in green, and rabbit pAb to GAPDH, 574, dilution 1:5,000, in red: [1] protein molecular weight standard of indicated molecular weight in kDa, [2] non transfected HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing tdTomato protein, [4] HEK293 cells transfected with pCI-Neo-mod vector expressing mCherry-HA protein. †he 8371 antibody recognizes tdTomato and mCherry proteins revealing major bands at about 60kDa and 30kDa, in green, respectfully. The tdTomato construct tested is identical to that found in several widely used expression vectors and contains two identical fluorescent modules, explaining why it is twice a large as the mCherry construct which contains only one fluorescent module. The red band at 37kDa corresponds to GAPDH protein used as a loading control.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

There are 181 publications in our database for this antibody or clone. Here are the latest 5, for more click below.


pmidtitleauthorscitation
36723624Lack of Paxillin phosphorylation promotes single-cell migration in vivoXue Q, Varady SRS, Waddell TQA, Roman MR, Carrington J, Roh-Johnson M.J Cell Biol. 2023 Mar 6;222(3):e202206078. doi: 10.1083/jcb.202206078. Epub 2023 Feb 1.
36609376Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2Xia B, Pan X, Luo RH, Shen X, Li S, Wang Y, Zuo X, Wu Y, Guo Y, Xiao G, Li Q, Long XY, He XY, Zheng HY, Lu Y, Pang W, Zheng YT, Li J, Zhang LK, Gao Z.Cell Discov. 2023 Jan 6;9(1):2. doi: 10.1038/s41421-022-00510-2.
36198703Intrinsic myocardial defects underlie an Rbfox-deficient zebrafish model of hypoplastic left heart syndromeHuang M, Akerberg AA, Zhang X, Yoon H, Joshi S, Hallinan C, Nguyen C, Pu WT, Haigis MC, Burns CG, Burns CE.Nat Commun. 2022 Oct 5;13(1):5877. doi: 10.1038/s41467-022-32982-x.
36175407MYPT1-PP1β phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesisTakahashi H, Yang G, Yoneshiro T, Abe Y, Ito R, Yang C, Nakazono J, Okamoto-Katsuyama M, Uchida A, Arai M, Jin H, Choi H, Tumenjargal M, Xie S, Zhang J, Sagae H, Zhao Y, Yamaguchi R, Nomura Y, Shimizu Y, Yamada K, Yasuda S, Kimura H, Tanaka T, Wada Y, Kodama T, Aburatani H, Zhu MS, Inagaki T, Osborne TF, Kawamura T, Ishihama Y, Matsumura Y, Sakai J.Nat Commun. 2022 Sep 29;13(1):5715. doi: 10.1038/s41467-022-33363-0.
36044862Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeletonWennagel D, Braz BY, Capizzi M, Barnat M, Humbert S.Cell Rep. 2022 Aug 30;40(9):111261. doi: 10.1016/j.celrep.2022.111261.

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

#
No results found

15 reviews for mouse anti-mCherry monoclonal antibody (1C51) 8371

  1. verified user

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Sheep Tissue sections (Brain)
  2. verified user

    Review details
    IHC – Wholemount
    Sample type:Zebrafish Embryo (To detect exogenous mCherry expression in brain)
  3. verified user

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Pig Tissue sections (Spinal cord)
  4. verified user

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Human Cell (U2OS)
  5. verified user

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Rat Tissue sections (Brain)
  6. verified user

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Mouse Tissue sections (Brain)
  7. verified user

    Review details
    Western blot
    Sample type:Human Cell lysate – whole cell (U2OS)
  8. verified user

    Review details
    Western blot
    Sample type:Fruit fly (Drosophila melanogaster) Tissue lysate – whole (Whole Head Lysate)
  9. verified user

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Rat Cell (Primary neurons)
  10. verified user

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Mouse Tissue sections (xenograft of mCherry labelled human (HUVEC) cells)
  11. verified user

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Rat Tissue sections (mCherry labeled smooth muscle cells in Bladder)
  12. verified user

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Mouse Tissue sections (mCherry overexpression in Mouse Hepatic Stellate C)
  13. verified user

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Rat Tissue sections (Brain)
  14. verified user

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Human Tissue sections (Pancreatic islet)
  15. verified user

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Human Cell (BT474 Cells)

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