| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG2a |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, IHC, WB |
| available sizes | 1 mg, 100 µg, 25 µg |
mouse anti-mCherry monoclonal antibody (1C51) 8371
$100.00 – $2,600.00
Antibody summary
- Mouse monoclonal to mCherry
- Suitable for: WB, ICC/IF, IHC
- Reacts with: tagged fusion proteins
- Isotype: IgG2a
- 100 µg, 25 µg, 1 mg
mouse anti-mCherry monoclonal antibody (1C51) 8371
| target relevance |
|---|
| Protein expression of mCherry and mCherry tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, mCherry fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody. Click for more on: epitope tags and mCherry |
| Biotechnology mCherry, a widely used fluorescent protein, has become an indispensable tool in various areas of scientific research. Derived from the red fluorescent protein DsRed, mCherry emits bright red fluorescence when excited with green light, making it an excellent choice for fluorescence microscopy and live-cell imaging studies. Researchers commonly utilize mCherry as a reporter gene, allowing them to track and visualize specific proteins, organelles, or cellular structures in real-time within living cells and organisms. Its compatibility with other fluorescent proteins enables dual-labeling experiments and multiplexing, providing valuable insights into dynamic cellular processes and interactions. Additionally, mCherry???s stability and resistance to photobleaching enhance its utility for long-term imaging studies. Moreover, mCherry has been employed in various fields, including cell biology, molecular biology, neuroscience, and developmental biology, where its fluorescence serves as a powerful tool to investigate cellular behavior, protein localization, and gene expression patterns. As research methodologies continue to advance, mCherry???s versatility and reliability continue to contribute significantly to our understanding of complex biological phenomena and pave the way for new discoveries in the life sciences. |
Data
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| Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA, construct, in red, and stained with mouse mAb to mCherry, 8371, dilution 1:500, in green. The blue is Hoechst staining of nuclear DNA. 8371 antibody reveals mCherry protein expressed only in transfected cells which appear golden in color. Untransfected cells do not react with the antibody, as a result only their nuclei are visible. |
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| Chromogenic immunostaining of formalin fixed paraffin embedded mCherry transfected monkey brain with mouse mAb to mCherry, 8371, dilution 1:2,000, detected in DAB (brown) following the ImmPress method. Hematoxylin (blue) was used as the counterstain. 8371 specifically detected the soma and axons of mCherry positive neurons in the cortex, as expected for this model. Mouse select image above for larger view.The sequence of the recombinant mCherry construct used to make this antibody is shown below;1 MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK 51 LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER 101 VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA 151 SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV 201 NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYKThe recombinant construct was expressed in and purified from E. coli. The sequence is identical to that found in a series of widely used expression vectors. |
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| Western blot analysis of HEK293 cell lysates using mouse mAb to mCherry, 8371, dilution 1:2,000, in green, and rabbit pAb to GAPDH, 574, dilution 1:5,000, in red: [1] protein molecular weight standard of indicated molecular weight in kDa, [2] non transfected HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing tdTomato protein, [4] HEK293 cells transfected with pCI-Neo-mod vector expressing mCherry-HA protein. †he 8371 antibody recognizes tdTomato and mCherry proteins revealing major bands at about 60kDa and 30kDa, in green, respectfully. The tdTomato construct tested is identical to that found in several widely used expression vectors and contains two identical fluorescent modules, explaining why it is twice a large as the mCherry construct which contains only one fluorescent module. The red band at 37kDa corresponds to GAPDH protein used as a loading control. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 181 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 36723624 | Lack of Paxillin phosphorylation promotes single-cell migration in vivo | Xue Q, Varady SRS, Waddell TQA, Roman MR, Carrington J, Roh-Johnson M. | J Cell Biol. 2023 Mar 6;222(3):e202206078. doi: 10.1083/jcb.202206078. Epub 2023 Feb 1. |
| 36609376 | Extracellular vesicles mediate antibody-resistant transmission of SARS-CoV-2 | Xia B, Pan X, Luo RH, Shen X, Li S, Wang Y, Zuo X, Wu Y, Guo Y, Xiao G, Li Q, Long XY, He XY, Zheng HY, Lu Y, Pang W, Zheng YT, Li J, Zhang LK, Gao Z. | Cell Discov. 2023 Jan 6;9(1):2. doi: 10.1038/s41421-022-00510-2. |
| 36198703 | Intrinsic myocardial defects underlie an Rbfox-deficient zebrafish model of hypoplastic left heart syndrome | Huang M, Akerberg AA, Zhang X, Yoon H, Joshi S, Hallinan C, Nguyen C, Pu WT, Haigis MC, Burns CG, Burns CE. | Nat Commun. 2022 Oct 5;13(1):5877. doi: 10.1038/s41467-022-32982-x. |
| 36175407 | MYPT1-PP1β phosphatase negatively regulates both chromatin landscape and co-activator recruitment for beige adipogenesis | Takahashi H, Yang G, Yoneshiro T, Abe Y, Ito R, Yang C, Nakazono J, Okamoto-Katsuyama M, Uchida A, Arai M, Jin H, Choi H, Tumenjargal M, Xie S, Zhang J, Sagae H, Zhao Y, Yamaguchi R, Nomura Y, Shimizu Y, Yamada K, Yasuda S, Kimura H, Tanaka T, Wada Y, Kodama T, Aburatani H, Zhu MS, Inagaki T, Osborne TF, Kawamura T, Ishihama Y, Matsumura Y, Sakai J. | Nat Commun. 2022 Sep 29;13(1):5715. doi: 10.1038/s41467-022-33363-0. |
| 36044862 | Huntingtin coordinates dendritic spine morphology and function through cofilin-mediated control of the actin cytoskeleton | Wennagel D, Braz BY, Capizzi M, Barnat M, Humbert S. | Cell Rep. 2022 Aug 30;40(9):111261. doi: 10.1016/j.celrep.2022.111261. |
Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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