mouse anti-DYDDDDK tag monoclonal antibody (1D2) 8963

Price range: $100.00 through $2,600.00

Antibody summary

Mouse monoclonal to DYDDDDK tag
Suitable for: WB, ICC/IF, ELISA
Reacts with: tagged fusion proteins
Isotype: IgG2b
100 µg, 25 µg, 1mg

available sizes

Clear

SKU: 8963parent Categories: antibody, epitope tag Tag: DYDDDDK tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-DYDDDDK tag monoclonal antibody (1D2) 8963

antibody
Database link: DYDDDDK tag (FLAG tag)
Tested applications WB,ICC/IF,ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with DYKDDDDK as determined by ELISA and western blot. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen Highly purified DYKDDDDK conjugated to KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG2b - Isotype Control

target relevance
DDDDK
Protein names FLAG tag
Alternative names FLAG tag, FLAG epitope, DYKDDDDK tag, DYDDDDK tag, FLAG peptide
Function The FLAG tag serves as an affinity and detection tag that can be fused to recombinant proteins. It is recognized by anti-FLAG antibodies and affinity reagents for purification, immunoprecipitation, western blotting, immunofluorescence, flow cytometry, and ELISA.
Description The FLAG tag is a short artificial peptide epitope widely used in recombinant protein expression, purification, detection, and localization studies. The canonical FLAG sequence is DYKDDDDK.
Biological role Recombinant protein detection Affinity purification Immunoprecipitation Protein localization studies
Research relevance FLAG-tagged proteins are extensively used in molecular biology and protein expression systems because the tag is small, highly immunogenic, and generally does not interfere with protein folding or function.
Keywords Epitope tag, Protein purification, Protein detection, Recombinant protein
Structure The FLAG tag is a synthetic peptide epitope with the canonical amino acid sequence DYKDDDDK.
Function FLAG serves as a molecular tag for detection, purification, and characterization of recombinant proteins.
Detection FLAG-tagged proteins are commonly detected using anti-FLAG antibodies such as M1 and M2 clones in western blotting, ELISA, immunofluorescence, and immunoprecipitation assays.
Purification FLAG affinity resins and magnetic beads enable rapid purification of FLAG-tagged proteins under native or denaturing conditions.
Research relevance The FLAG tag is one of the most widely used epitope tags in molecular biology, biotechnology, and recombinant protein production.

Data

Monoclonal Mouse anti-FLAG-Tag Antibody recognizes N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins. Validated for use in Western Blot and ELISA assays.

FAQ & Publications

Frequently Asked Questions

What applications is the mouse anti-DYDDDDK tag monoclonal antibody (1D2) validated for?

This antibody is validated for use in Western Blot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), and ELISA applications.

How should the mouse anti-DYDDDDK tag monoclonal antibody be stored to maintain its stability?

The antibody should be stored at 2-8°C in a storage buffer consisting of PBS, pH 7.2, with 0.09% sodium azide (NaN₃) to ensure stability.

What is the immunogen used to generate the mouse anti-DYDDDDK tag monoclonal antibody (1D2)?

The immunogen is highly purified DYKDDDDK peptide conjugated to keyhole limpet hemocyanin (KLH).

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.