| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | chicken |
| isotype | IgY |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, IHC, WB |
| available sizes | 1 mg, 100 µg, 25 µg |
chicken anti-Beta Galactosidase polyclonal antibody 1779
$100.00 – $2,600.00
Antibody summary
- Chicken polyclonal to βGal
- Suitable for: WB, ICC/IF, IHC
- Reacts with: tagged fusion proteins
- Isotype: IgY
- 100 µg, 25 µg, 1 mg
chicken anti-Beta Galactosidase polyclonal antibody 1779
| antibody |
|---|
| Database link: P00722 |
| Tested applications WB,ICC/IF,IHC |
| Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test. |
| Immunogen Chickens were immunized with highly purified beta-galactosidase from Escherichia col |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3 |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgY |
| Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720 |
| Isotype control Chicken polyclonal - Isotype Control |
| target relevance |
|---|
| Protein expression of beta-galactosidase and bGal tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, bGal presence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody. Click for more on: epitope tags and bGal |
| Protein names Beta-galactosidase (Beta-gal) (EC 3.2.1.23) (Lactase) |
| Gene names b0344 JW0335,lacZ b0344 JW0335 |
| Protein family Glycosyl hydrolase 2 family |
| Mass 116483Da |
| Catalytic activity CATALYTIC ACTIVITY: Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.; EC=3.2.1.23; |
| Structure SUBUNIT: Homotetramer. {ECO:0000269|PubMed:11045615, ECO:0000269|PubMed:11732897, ECO:0000269|PubMed:14621996, ECO:0000269|PubMed:8008071}. |
| Target Relevance information above includes information from UniProt accession : P00722 |
| The UniProt Consortium |
Data
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| Immunofluorescence cell staining- chicken anti-Beta Galactosidase polyclonal antibody 1779 Immunofluorescent analysis of 4% paraformaldehyde (PFA) – fixed, permeabilized with 0.1% triton X-100 CHO cells transfected with beta galactosidase constructs (CHO-BGAL) labeling beta galactosidase with antibody #1779 at 1/1000 dilution (1µg/mL), followed by Donkey Anti-chicken IgY, Alexa 594 conjugate at 1/1000 (1µg/mL) (red). Nucleus was counterstained with DAPI (blue). Also shown staining in parental cell line. |
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Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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