rabbit anti-6X His tag polyclonal antibody 7094

Name: rabbit anti-6X His tag polyclonal antibody 7094
SKU: 7094parent
Availability: InStock
Rating: 4.46 (24 reviews)

Rated 4.46 out of 5 based on 24 customer ratings

$100.00 – $2,600.00

Antibody summary

Rabbit polyclonal to 6X His tag
Suitable for: ELISA
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

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SKU: 7094parent Categories: antibody, epitope tag Tag: 6XHis tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-6X His tag polyclonal antibody 7094

antibody
Database link: 6x His tag sequence HHHHHH
Tested applications ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with HHHHHH as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen Highly purified HHHHHH conjugated with KLH (6xHIS)
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance

6X His tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify 6X His tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, 6X His tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and 6x His tag

Biotechnology
The 6x Histidine (6x His) tag has become an indispensable tool in both biotechnology and scientific research, particularly in the field of recombinant protein expression and purification. The 6x His tag consists of six consecutive histidine residues genetically fused to a protein of interest. This small peptide sequence offers several advantages, making it highly desirable for the generation of fusion proteins. One of its main benefits is its ability to bind to immobilized metal ions, such as nickel or cobalt, with high affinity. This property allows for efficient and selective purification of the fusion protein through metal affinity chromatography, simplifying the protein purification process and yielding highly pure and concentrated protein samples. Furthermore, the 6His tag also facilitates easy detection and quantification of the fusion protein using anti-His antibodies in techniques like Western blotting. The versatility and simplicity of the 6x His tag have significantly advanced research efforts in protein expression, characterization, and functional analysis, allowing researchers to explore the structure and function of a wide range of proteins in diverse biological systems.

Data

rabbit anti-6X His tag polyclonal antibody 7094 1:1000 (1µg/mL) and donkey anti-rabbit IgG (H&L) alexa fluor 594

Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilized with 0.1% triton X-100 in CHO cells transfected with 12 tags construct (CHO-12 tags) labeling 6xHIS with rabbit-anti-6x-his tag-polyclonal antibody 7094 1/1000 dilution (1µg/mL), followed by donkey anti-rabbit IgG (H&L), Alexa fluor 594 antibody at 1/500 dilution (4µg/mL) (red). Nucleus was counterstained with DAPI (blue). Bottom panel shows CHO parental cell line staining.

Publications

pmid	title	authors	citation
36339259	Activity-based probes trap early active intermediates during metacaspase activation.	Vida �trancar, Katarina P van Midden, Daniel Krahn, Kyoko Morimoto, Marko Novinec, Christiane Funk, Simon Stael, Christopher J Schofield, Marina Klemen?i?, Renier A L van der Hoorn	iScience 25:105247
35986286	Modulation of macrophage inflammatory function through selective inhibition of the epigenetic reader protein SP140.	Mohammed Ghiboub, Jan Koster, Peter D Craggs, Andrew Y F Li Yim, Anthony Shillings, Sue Hutchinson, Ryan P Bingham, Kelly Gatfield, Ishtu L Hageman, Gang Yao, Heather P O'Keefe, Aaron Coffin, Amish Patel, Lisa A Sloan, Darren J Mitchell, Thomas G Hayhow, Laurent Lunven, Robert J Watson, Christopher E Blunt, Lee A Harrison, Gordon Bruton, Umesh Kumar, Natalie Hamer, John R Spaull, Danny A Zwijnenburg, Olaf Welting, Theodorus B M Hakvoort, Anje A Te Velde, Johan van Limbergen, Peter Henneman, Rab K Prinjha, Menno P J de Winther, Nicola R Harker, David F Tough, Wouter J de Jonge	BMC Biol 20:182
35903773	Human SLC4A11 does not complement BOR1 or support borate transport in Saccharomyces cerevisiae.	Jean L Beltran, Richara K Bain, Marie J Stiefel, Alexis S McDonnell, Natalie N Carr, Bryan H Thurtle-Schmidt	MicroPubl Biol 2022:N/A
35584627	Broad and ultra-potent cross-clade neutralization of HIV-1 by a vaccine-induced CD4 binding site bovine antibody.	Behnaz Heydarchi, Danielle S Fong, Hongmei Gao, Natalia A Salazar-Quiroz, Jack M Edwards, Christopher A Gonelli, Samantha Grimley, Turgut E Aktepe, Charlene Mackenzie, William J Wales, Marit J van Gils, Albert Cupo, Isabelle Rouiller, Paul R Gooley, John P Moore, Rogier W Sanders, David Montefiori, Ashish Sethi, Damian F J Purcell	Cell Rep Med 3:100635
35443160	A dual-function phage regulator controls the response of cohabiting phage elements via regulation of the bacterial SOS response.	Gil Azulay, Anna Pasechnek, Olga Stadnyuk, Shai Ran-Sapir, Ana Mejia Fleisacher, Ilya Borovok, Nadejda Sigal, Anat A Herskovits	Cell Rep 39:110723

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.