| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | rabbit |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA |
| available sizes | 1 mg, 100 µg, 25 µg |
rabbit anti-V5 tag polyclonal antibody 2475
$100.00 – $2,600.00
Antibody summary
- Rabbit polyclonal to V5 tag
- Suitable for: ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG
- 100 µg, 25 µg, 1 mg
rabbit anti-V5 tag polyclonal antibody 2475
| target relevance |
|---|
| V5 tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify V5tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, V5 tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody. Click for more on: epitope tags and V5 tag |
| Biotechnology The V5 tag has become a valuable tool in scientific research, particularly in studies involving the expression and detection of recombinant proteins. The V5 tag is a short peptide sequence (GKPIPNPLLGLDST) that can be fused to the N or C terminus of a target protein, allowing for easy detection and purification. Researchers commonly utilize the V5 tag as an epitope for specific V5 tag antibodies, enabling convenient and sensitive visualization of the tagged protein in various experimental assays, including Western blotting and immunofluorescence. The small size of the V5 tag minimizes potential interference with the function and properties of the tagged protein, making it an attractive choice as a molecular tag. Additionally, the V5 tag can be used for protein purification using affinity chromatography, facilitating the isolation and characterization of the tagged protein. The widespread use of the V5 tag in research owes to its efficiency, reliability, and compatibility with various expression systems, making it an essential tool for investigating protein dynamics, interactions, and cellular localization in diverse biological contexts. |
Data
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Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| ELISA |
Documents
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