rabbit anti-V5 tag polyclonal antibody 2475

$100.00 – $2,600.00

Antibody summary

Rabbit polyclonal to V5 tag
Suitable for: ELISA
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 2475parent Categories: antibody, epitope tag Tag: V5

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	rabbit
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

rabbit anti-V5 tag polyclonal antibody 2475

antibody
Database link: V5 tag sequence -GKPIPNPLLGLDST Derived from V5 95-108. Example strain H3N8 P11208
Tested applications ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with V5 as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen Highly purified V5 peptide conjugated with KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance

V5 tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify V5tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, V5 tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and V5 tag

Biotechnology
The V5 tag has become a valuable tool in scientific research, particularly in studies involving the expression and detection of recombinant proteins. The V5 tag is a short peptide sequence (GKPIPNPLLGLDST) that can be fused to the N or C terminus of a target protein, allowing for easy detection and purification. Researchers commonly utilize the V5 tag as an epitope for specific V5 tag antibodies, enabling convenient and sensitive visualization of the tagged protein in various experimental assays, including Western blotting and immunofluorescence. The small size of the V5 tag minimizes potential interference with the function and properties of the tagged protein, making it an attractive choice as a molecular tag. Additionally, the V5 tag can be used for protein purification using affinity chromatography, facilitating the isolation and characterization of the tagged protein. The widespread use of the V5 tag in research owes to its efficiency, reliability, and compatibility with various expression systems, making it an essential tool for investigating protein dynamics, interactions, and cellular localization in diverse biological contexts.

Data

ELISA-image-rabbit-anti-V5 tag-polyclonal-antibody-2475

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.