Skip to content

mouse anti-mCherry monoclonal antibody (5A6) 6575

$100.00$2,600.00

Antibody summary

  • Mouse monoclonal to mCherry
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: tagged fusion proteins
  • Isotype: IgG1
  • 100 µg, 25 µg, 1 mg
SKU: 6575parent Categories: , Tags: ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

available sizes

1 mg, 100 µg, 25 µg

Available product – mouse anti-mCherry monoclonal antibody (5A6) 6575

antibody
Database link:
mCherry X5DSL3
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:2000 IF/IHC: 1:500
Immunogen
Full length recombinant mCherry protein expressed in and purified from E. coli
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
affinity purified
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein expression of mCherry and mCherry tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, mCherry fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody.

Click for more on: epitope tags and mCherry
Biotechnology
mCherry, a widely used fluorescent protein, has become an indispensable tool in various areas of scientific research. Derived from the red fluorescent protein DsRed, mCherry emits bright red fluorescence when excited with green light, making it an excellent choice for fluorescence microscopy and live-cell imaging studies. Researchers commonly utilize mCherry as a reporter gene, allowing them to track and visualize specific proteins, organelles, or cellular structures in real-time within living cells and organisms. Its compatibility with other fluorescent proteins enables dual-labeling experiments and multiplexing, providing valuable insights into dynamic cellular processes and interactions. Additionally, mCherry’s stability and resistance to photobleaching enhance its utility for long-term imaging studies. Moreover, mCherry has been employed in various fields, including cell biology, molecular biology, neuroscience, and developmental biology, where its fluorescence serves as a powerful tool to investigate cellular behavior, protein localization, and gene expression patterns. As research methodologies continue to advance, mCherry’s versatility and reliability continue to contribute significantly to our understanding of complex biological phenomena and pave the way for new discoveries in the life sciences.

Data

ICC/IF-image-mouse-anti-mCherry-monoclonal-antibody-5A6-6575
Immunofluorescent analysis of HEK293 cells stably transduced with a lentiviral vector expressing mCherry-HA construct (red) and stained with mouse mAb to mCherry, 6575, dilution 1:500 in green. The blue is Hoechst staining of nuclear DNA. The 6575 antibody reveals the mCherry protein expressed only in transduced cells which appear golden in color. Untransduced cells expressing no mCherry are not recognized by the 6575 antibody and so only their nuclei are visible.
WB-image-mouse-anti-mCherry-monoclonal-antibody-5A6-6575
Western blot analysis of HEK293 whole cell lysates using mouse mAb to mCherry, 6575, dilution 1:2,000, in green [1] protein standard, [2] HEK293, [3] HEK293 cells stably transduced with lenti-virus containing mCherry-HA construct, and [4] HEK293 cells transfected with mCherry-HAI construct. Major band at about 28kDa corresponds to mCherry protein. The band at 20kDa corresponds to cleaved form of mCherry protein.This antibody was made against a recombinant construct expressed in and purified from E. coli. The sequence is identical to that found in a series of widely used expression vectors.1 MVSKGEEDNM AIIKEFMRFK VHMEGSVNGH EFEIEGEGEG RPYEGTQTAK51 LKVTKGGPLP FAWDILSPQF MYGSKAYVKH PADIPDYLKL SFPEGFKWER101 VMNFEDGGVV TVTQDSSLQD GEFIYKVKLR GTNFPSDGPV MQKKTMGWEA151 SSERMYPEDG ALKGEIKQRL KLKDGGHYDA EVKTTYKAKK PVQLPGAYNV201 NIKLDITSHN EDYTIVEQYE RAEGRHSTGG MDELYKEpitope mapping of 6575 was performed using a nested set of 20 amino acid peptides covering the whole sequence and overlapping by 5 amino acids. The peptide highlighted above strongly inhibited binding of 6575 to mCherry, while the previous and following peptides had no effect suggesting that the central 10 amino acids, KLKDGGHYDA are a key component of antibody binding.
IHC-image-mouse-anti-mCherry-monoclonal-antibody-5A6-6575
Chromogenic Immunostaining of formalin fixed paraffin embedded mCherry transfected monkey brain with mouse mAb to mCherry, 6575, dilution 1:2,000, detected with DAB (brown) following the Vector labs Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. 6575 specifically detected the soma and axons of mCherry positive neurons in the cerebellum, as expected for this model. Mouse select image for larger view.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation
11050231Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine)Heikal AA, Hess ST, Baird GS, Tsien RY, Webb WW.Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11996-2001. doi: 10.1073/pnas.97.22.11996.
11050230The structure of the chromophore within DsRed, a red fluorescent protein from coralGross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY.Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11990-5. doi: 10.1073/pnas.97.22.11990.
11050229Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coralBaird GS, Zacharias DA, Tsien RY.Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11984-9. doi: 10.1073/pnas.97.22.11984.
10504696Fluorescent proteins from nonbioluminescent Anthozoa speciesMatz MV, Fradkov AF, Labas YA, Savitsky AP, Zaraisky AG, Markelov ML, Lukyanov SA.Nat Biotechnol. 1999 Oct;17(10):969-73. doi: 10.1038/13657.
8303295Green fluorescent protein as a marker for gene expressionChalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC.Science. 1994 Feb 11;263(5148):802-5. doi: 10.1126/science.8303295.

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.