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mouse anti-GFP monoclonal antibody (1F1) 7157

$100.00$2,600.00

Antibody summary

  • Mouse monoclonal to GFP
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: tagged fusion proteins
  • Isotype: IgM
  • 100 µg, 25 µg, 1 mg
SKU: 7157parent Categories: , Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgM

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

available sizes

1 mg, 100 µg, 25 µg

mouse anti-GFP monoclonal antibody (1F1) 7157

antibody
Database link:
P42212
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:1000, IF/ICC 1:1000
Immunogen
prot-r-AcGFP recombinant protein purified from E. coli. The epitope is in the N-terminal 18 amino acids of the protein, the peptide MVSKGAELFTGIVPILIE, which is found in the Clontech and other GFP vectors
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
affinity purified
Clonality
monoclonal
Isotype
IgM
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgM- Isotype Control
target relevance
Protein expression of GFP and GFP tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, GFP fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody.

Click for more on: epitope tags and GFP
Protein names
Green fluorescent protein
Gene names
GFP,GFP
Protein family
GFP family
Mass
26886Da
Function
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Tissues
Photocytes.
Structure
Monomer.
Post-translational modification
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnology
Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Green fluorescent protein can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when Thr-203 is replaced by Tyr). Further generation of mutants led to more stable proteins (at 37 degrees Celsius for example) with brighter fluorescence and longer fluorescence lifetimes. Fluorescent proteins and their mutated allelic forms have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions (PubMed:17685514, PubMed:17685554, PubMed:8578587, PubMed:8707053, PubMed:9145105, PubMed:9154981, PubMed:9759496, PubMed:9782051). Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy (PubMed:17685514).
Target Relevance information above includes information from UniProt accession : P42212
The UniProt Consortium

Data

ICC/IF-image-mouse-anti-GFP-monoclonal-antibody-1F1-7157
Immunofluorescent analysis of transfected HEK293 cells transfected with a GFP construct and stained with mouse mAb to GFP, 7157, dilution 1:1,000, in red. The blue is Hoechst staining of nuclear DNA. The 7157 antibody binds to GFP protein expressed only in transfected cells, and as a result cells are appeared in orange-golden color.
IHC-image-mouse-anti-GFP-monoclonal-antibody-1F1-7157
PROT-r-AcGFP1 MVSKGAELFT GIVPILIELN GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT 61 LVTTLSYGVQ CFSRYPDHMK QHDFFKSAMP EGYIQERTIF FEDDGNYKSR AEVKFEGDTL 121 VNRIELTGTD FKEDGNILGN KMEYNYNAHN VYIMTDKAKN GIKVNFKIRH NIEDGSVQLA 181 DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMI YFGFVTAAAI THGMDELYKChromogenic immunostaining of a 4% PFA fixed paraffin embedded section from a GFP expressing transgenic mouse brain stained with mouse mAb to GFP, 7157, dilution 1:4,000, detected in DAB (brown) following the Vector Labs Mouse on Mouse (M.O.M.®) ImmPRESS® HRP method with citra buffer retrieval. with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. This GFP antibody specifically labeled cells in the cerebellum as expected. Mouse select image for larger view.
Western_blot-image-mouse-anti-GFP-monoclonal-antibody-1F1-7157
Western blot analysis of transfected and control HEK293 cell lysates using mouse mAb to GFP, 7157, in green, dilution 1:1,000: [1] protein standard, [2] Control, non-transfected cells, [3] cells transfected with an mCherry red fluorescent protein construct and [4] cells transfected with GFP construct. The strong green band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct, the antibody does not bind to mCherry. The same blot was simultaneously probed with chicken pAb to HSP60, 7157, dilution 1:10,000, in red. The single band at 60kDa represents the HSP60 protein expressed in all preparations.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

There are 9 publications in our database for this antibody or clone. Here are the latest 5, for more click below.


pmidtitleauthorscitation
13911999Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, AequoreaSHIMOMURA O, JOHNSON FH, SAIGA Y.J Cell Comp Physiol. 1962 Jun;59:223-39. doi: 10.1002/jcp.1030590302.
12693991A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutantsGurskaya NG, Fradkov AF, Pounkova NI, Staroverov DB, Bulina ME, Yanushevich YG, Labas YA, Lukyanov S, Lukyanov KA.Biochem J. 2003 Jul 15;373(Pt 2):403-8. doi: 10.1042/BJ20021966.
11988576Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cellsZacharias DA, Violin JD, Newton AC, Tsien RY.Science. 2002 May 3;296(5569):913-6. doi: 10.1126/science.1068539.
9759496The green fluorescent proteinTsien RY.Annu Rev Biochem. 1998;67:509-44. doi: 10.1146/annurev.biochem.67.1.509.
8703075Crystal structure of the Aequorea victoria green fluorescent proteinOrmö M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ.Science. 1996 Sep 6;273(5280):1392-5. doi: 10.1126/science.273.5280.1392.

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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