mouse anti-6X His tag monoclonal antibody (7B8) 1955

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to 6X His tag
Suitable for: ELISA
Reacts with: tagged fusion proteins
Isotype: IgG2b
100 µg, 25 µg, 1 mg

SKU: 1955parent Categories: antibody, epitope tag Tag: 6XHis tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-6X His tag monoclonal antibody (7B8) 1955

antibody
Database link: 6x His tag sequence HHHHHH
Tested applications ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with 6xHIS as determined by ELISA and western blot. Optimal working dilutions should be determined experimentally by the investigator
Immunogen Highly purified HHHHHH conjugated with KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG2b - Isotype Control

target relevance

6X His tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify 6X His tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, 6X His tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and 6x His tag

Biotechnology
The 6x Histidine (6x His) tag has become an indispensable tool in both biotechnology and scientific research, particularly in the field of recombinant protein expression and purification. The 6x His tag consists of six consecutive histidine residues genetically fused to a protein of interest. This small peptide sequence offers several advantages, making it highly desirable for the generation of fusion proteins. One of its main benefits is its ability to bind to immobilized metal ions, such as nickel or cobalt, with high affinity. This property allows for efficient and selective purification of the fusion protein through metal affinity chromatography, simplifying the protein purification process and yielding highly pure and concentrated protein samples. Furthermore, the 6His tag also facilitates easy detection and quantification of the fusion protein using anti-His antibodies in techniques like Western blotting. The versatility and simplicity of the 6x His tag have significantly advanced research efforts in protein expression, characterization, and functional analysis, allowing researchers to explore the structure and function of a wide range of proteins in diverse biological systems.

The expression of 6XHIS was determined by Western blot. CHO cells were transfected with pcDNA3-12 Tags (CHO-12 Tag). Whole cell lysates of CHO-12 Tag, and CHO were loaded at 2µg and 0.2µg then incubated with mouse anti-6X His tag monoclonal antibody (7B8) 1955 at 1:10000 (dilution 0.1 µg/mL) for 1h at room temperature.

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.