Chemokine Induced Migration Cells expressing recombinant CXCR1 were assayed for migration through the transwell bare filter at various concentrations of IL-8. The response is expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

CXCL8-Biotin Detection on PAGE Gel Detection of various amounts (1-62 ng) of Biotinylated-CXCL8 with streptavidin conjugated to 800CW dye (LiCOR).

Biotinylated Interleukin 8 (IL-8 /CXCL8) 8024

$134.00 – $2,862.00

Summary

Expression: E.coli
Amino Acid Range: 28-99

SKU: 8024parent Categories: chemokine, proteins Tag: CXCL8 (IL-8)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P10145
express system	E.coli
product tag	biotin at C-terminal
purity	> 97% by SDS PAGE
molecular weight	Predicted Molecular Mass: 10,803.49 Da Extinction Coefficient: 12,900 M-1 cm-1 Actual Molecular Mass: 10,803 Da by ESI Mass Spec
available size	10 µg, 100 µg, 2 µg, 50 µg
endotoxin	<0.01 EU per 1μg of the protein by the LAL method

Biotinylated Interleukin 8 (IL-8 /CXCL8) 8024

protein
Database link: human P10145
Size and concentration 2, 10, 50, 100µg and lyophilized
Form Lyophilized
Storage Instructions Avoid repeated freeze-thaw cycles: • 12 months from date of receipt, -20 to -70 °C as supplied. • 1 month, 2 to 8 °C under sterile conditions after reconstitution. • 3 months, -20 to -70 °C under sterile conditions after reconstitution
Storage buffer Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100µg/mL in sterile water. Shipping: Room Temp
Purity > 97% by SDS PAGE and HPLC

target relevance
Interleukin 8 (IL-8 )(CXCL8) is secreted primarily by macrophages and monocytes. It is one of the key mediators for inflammatory responses. IL-8 is a strong chemotractant for neutrophils and monoccytes, and promotes activation of these target cells by binding to two cell surface receptors CXCR1 and CXCR2. It is also a strong angiogenic agent, and is considered to play a role in the pathogenesis of bronchiolitis.Biotinylated CXCL8 is made using the enzymatic method, which has several advantages over chemical biotinylation methods. The attachment of biotin at a specific lysine residue is nearly 100% complete, and leads to a modified chemokine with functionalities comparable to those of the unmodified CXCL8 in migration assay. Utilizing avidin/streptavidin analogues conjugated to various fluorescent labels, biotinylated CXCL8 is useful in studies on receptor identification, distribution, chemokine binding, and other cellular assays. They serve as great tools in visualization and quantification, and can replace radioactively labeled chemokines.
Protein names Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [C
Gene names CXCL8,CXCL8 IL8
Protein family Intercrine alpha (chemokine CxC) family
Mass 11098Da
Function Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:18692776, PubMed:7636208). Also plays an important role in neutrophil activation (PubMed:2145175, PubMed:9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed:1840701, PubMed:1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed:11971003, PubMed:8662698).
Subellular location Secreted.
Structure Homodimer (PubMed:31235521). Dimer formation is disrupted by tick evasin-3 (PubMed:31235521). Interacts with TNFAIP6 (via Link domain); this interaction interferes with chemokine binding to glycosaminoglycans.
Post-translational modification Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.; Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.; (Microbial infection) Cleaved by group A Streptococcus protease SpyCEP; leading to impaired neutrophil endothelial transmigration and thus increased virulence.
Target Relevance information above includes information from UniProt accession: P10145
The UniProt Consortium

Data

Chemokine Induced Migration Cells expressing recombinant CXCR1 were assayed for migration through the transwell bare filter at various concentrations of IL-8. The response is expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

CXCL8-Biotin Detection on PAGE Gel Detection of various amounts (1-62 ng) of Biotinylated-CXCL8 with streptavidin conjugated to 800CW dye (LiCOR).

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.