Migration Assay: Cells expressing recombinant CCR1 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated HCC-1. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

Biotinylated Hemofiltrate CC chemokine-1 (HCC-1/CCL14) 8021

Price range: $134.00 through $3,368.00

Summary

Expression: E.coli
Amino Acid Range: 28-93

available size

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SKU: 8021parent Categories: chemokine, proteins Tag: CCL14 (HCC-1)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P16627
express system	E.coli
product tag	biotin at C-terminal
purity	> 97% by SDS PAGE
molecular weight	Predicted Molecular Mass: 10,219.5471 Da Extinction Coefficient: 19,300 M-1 cm-1 Actual Molecular Mass: 10,219.5471 Da by ESI Mass Spec
available size	10 µg, 100 µg, 2 µg, 50 µg
endotoxin	<0.01 EU per 1μg of the protein by the LAL method

Biotinylated Hemofiltrate CC chemokine-1 (HCC-1/CCL14) 8021

Data

Migration Assay: Cells expressing recombinant CCR1 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated HCC-1. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

FAQ & Publications

Frequently Asked Questions

What is the expression system used for producing Biotinylated Hemofiltrate CC chemokine-1 (HCC-1/CCL14)?

The Biotinylated Hemofiltrate CC chemokine-1 (HCC-1/CCL14) is expressed using an Escherichia coli (E.coli) expression system.

How should I store the lyophilized Biotinylated HCC-1 protein to maintain its stability?

The lyophilized protein should be stored at -20 to -70 °C as supplied, which preserves stability for up to 12 months. After reconstitution, it can be stored for 1 month at 2 to 8 °C under sterile conditions or for 3 months at -20 to -70 °C under sterile conditions. Avoid repeated freeze-thaw cycles to maintain protein integrity.

What is the purity level of this Biotinylated HCC-1 protein preparation?

The Biotinylated HCC-1 protein preparation has a purity greater than 97% as determined by SDS-PAGE and HPLC analysis.

Is the Biotinylated HCC-1 protein endotoxin-free, and how is this measured?

Yes, the endotoxin level is less than 0.01 endotoxin units (EU) per microgram of protein, as measured by the Limulus Amebocyte Lysate (LAL) assay method.

What biological activities are associated with the active form of HCC-1 after N-terminal processing?

The active form of HCC-1, generated by N-terminal processing via the uPA-plasmin system, is a potent agonist for chemokine receptors CCR1, CCR5, and to a lesser extent CCR3. It induces chemotaxis of various leukocyte types and has been shown to inhibit HIV entry effectively.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.