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Biotinylated Human CCL5 (Rantes) 8019

$134.00$2,862.00

Summary

  • Expression: E.coli
  • Amino Acid Range: 24-91
SKU: 8019parent Categories: , Tags: ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P13501

express system

E.coli

product tag

biotin at C-terminal​

purity

> 97% by SDS PAGE

molecular weight

Predicted Molecular Mass: 10,269.7258 Da Extinction Coefficient: 18,020 M-1 cm-1 Actual Molecular Mass: 10,269.7258 Da by ESI Mass Spec

available size

10 µg, 100 µg, 2 µg, 50 µg

endotoxin

<0.01 EU per 1μg of the protein by the LAL method

Biotinylated human CCL5 (Rantes) 8019

protein
Database link:
human P13501
Size and concentration
2, 10, 50, 100µg and lyophilized
Form
Lyophilized
Storage Instructions
Avoid repeated freeze-thaw cycles:
• 12 months from date of receipt, -20 to -70 °C as supplied.
• 1 month, 2 to 8 °C under sterile conditions after reconstitution.
• 3 months, -20 to -70 °C under sterile conditions after reconstitution
Storage buffer
​Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100µg/mL in sterile water.
Shipping: Room Temp
Purity
> 97% by SDS PAGE and HPLC
target relevance
Regulated on Activation, Normal T cell Expressed and Secreted (RANTES) (CCL5) is a proinflammatory chemokine that induces migration and activation of leukocytes, as well as implication in HIV infection. It binds to cell surface receptors CCR1, CCR3, CCR4, and CCR5. Its biological effects on leukocyte activation and HIV infection displays dependence on concentration and on the binding of cell surface glycosaminoglycans.
Protein names
C-C motif chemokine 5 (EoCP) (Eosinophil chemotactic cytokine) (SIS-delta) (Small-inducible cytokine A5) (T cell-specific protein P228) (TCP228) (T-cell-specific protein RANTES) [Cleaved into: RANTES(3-68); RANTES(4-68)]
Gene names
CCL5,CCL5 D17S136E SCYA5
Protein family
Intercrine beta (chemokine CC) family
Mass
9990Da
Function
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) (PubMed:1380064, PubMed:15923218, PubMed:16791620, PubMed:8525373, PubMed:9516414). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485).
Subellular location
Secreted.
Tissues
Expressed in the follicular fluid (at protein level). T-cell and macrophage specific.
Structure
Homo and heterooligomers with other chemokines. Interacts with the brown dog tick evasin-4.
Post-translational modification
N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.; N-terminal processed form RANTES(4-68) is produced by proteolytic cleavage by cathepsin CTSG.; The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
Target Relevance information above includes information from UniProt accession: P13501
The UniProt Consortium

Data

Migration Assay: Cells expressing recombinant CCR5 were assayed for migration through a transwell filter at various concentrations of WT Rantes or Biotinylated Rantes. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).
Migration Assay: Cells expressing recombinant CCR5 were assayed for migration through a transwell filter at various concentrations of WT Rantes or Biotinylated Rantes. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Migration assay

Documents

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