Migration Assay: Cells expressing recombinant CCR2 were assayed for migration through a transwell filter at various concentrations of MCP-1. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated). Activity: EC50 = 1.26nM determined by migration assay of THP-1 Cells

20nM Biotinylated CCL2 with Streptavidin PE on THP-1 Cell Flow Cytometry: Uptake of biotin-CCL2 by THP1 cells (dark green) is abolished by the addition of CCL7 (orange) but not CCL14 (light green), suggesting a CCR2-specific internalization. THP1 cells are not stained in the absence of biotin-CCL2-biotin (cyan).

Biotinylated Human CCL2 (MCP-1) 6003

$134.00 – $3,368.00

Summary

Expression: E.coli
Amino Acid Range: 24-99

SKU: 6003parent Categories: chemokine, proteins Tag: CCL2 (MCP-1)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P13500
express system	E.coli
product tag	biotin at C-terminal
purity	> 97% by SDS PAGE
molecular weight	Predicted Molecular Mass: 11.085 kDa Extinction Coefficient: 14,180 M-1 cm-1 Actual Molecular Mass: 11.085 kDa by ESI MS
available size	10 µg, 100 µg, 2 µg, 50 µg
endotoxin	<0.01 EU per 1μg of the protein by the LAL method

Biotinylated Human CCL2 (MCP-1) 6003

protein
Database link: human P13500
Size and concentration 2, 10, 50, 100µg and lyophilized
Form Lyophilized
Storage Instructions Avoid repeated freeze-thaw cycles: • 12 months from date of receipt, -20 to -70 °C as supplied. • 1 month, 2 to 8 °C under sterile conditions after reconstitution. • 3 months, -20 to -70 °C under sterile conditions after reconstitution
Storage buffer Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100µg/mL in sterile water. Shipping: Room Temp
Purity > 97% by SDS PAGE and HPLC

target relevance
Monocyte chemoattractant protein-1 (MCP-1/CCL2) is produced by many cell types at sites of inflammation. It regulates chemotaxis and transendothielial migration of monocytes, as well as memory T cells and natural killer cells by interacting with their membrane surface receptor CCR2. MCP-1 has also been implicated in a number of disease states, such as rheumatoid arthritis, atherosclerosis, autoimmune diseases, tumor progression, and HIV infection. This cytokine displays activity for monocytes and basophils but not for neutrophils or eosinophils. It binds to chemokine receptors CCR2 and CCR4.Biotinylated CCL2 is made using the enzymatic method, which has several advantages over chemical biotinylation methods. The attachment of biotin at a specific lysine residue within the C-terminal avi tag sequence is nearly 100% complete, and leads to a modified chemokine with functionalities comparable to those of the unmodified CCL2 in migration assay. Combining with avidin analogues conjugated to various fluorescent labels, biotinylated CCL2 is useful in studies on receptor identification, distribution, chemokine binding, and other cellular assays. They serve as great tools in visualization and quantification, and replace the needs for radioactively labeled chemokines.
Protein names C-C motif chemokine 2 (HC11) (Monocyte chemoattractant protein 1) (Monocyte chemotactic and activating factor) (MCAF) (Monocyte chemotactic protein 1) (MCP-1) (Monocyte secretory protein JE) (Small-inducible cytokine A2)
Gene names CCL2,CCL2 MCP1 SCYA2
Protein family Intercrine beta (chemokine CC) family
Mass 9606Da
Function Acts as a ligand for C-C chemokine receptor CCR2 (PubMed:10529171, PubMed:10587439, PubMed:9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:10587439, PubMed:9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8195247, PubMed:8627182, PubMed:9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690).
Subellular location Secreted .
Tissues Expressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level) (PubMed:23765988). Expressed in monocytes (PubMed:2513477).
Structure Monomer or homodimer; in equilibrium (PubMed:15033992, PubMed:8898111, PubMed:8989326, PubMed:9837883). Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans (PubMed:9792674). Interacts with TNFAIP6 (via Link domain).
Post-translational modification Processing at the N-terminus can regulate receptor and target cell selectivity (PubMed:8627182). Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant (PubMed:8627182).; N-Glycosylated.
Target Relevance information above includes information from UniProt accession: P13500
The UniProt Consortium

Data

No results found

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.