| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 1 mg, 100 µg, 25 µg |
mouse anti-DYDDDDK tag monoclonal antibody (FG4R) 5299
$100.00 – $2,600.00
Antibody summary
- Mouse monoclonal to DYDDDDK tag
- Suitable for: WB,ICC/IF,ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG1
- 100 µg, 25 µg, 1 mg
mouse anti-DYDDDDK tag monoclonal antibody (FG4R) 5299
| target relevance |
|---|
| DYDDDDK (Flag) tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify DYDDDDK (Flag) tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, DYDDDDK (Flag) tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody. Click for more on: epitope tags and DYDDDDK (Flag) tag |
| Biotechnology The DYDDDDK tag, also known as the FLAG tag, is a short peptide sequence that has become a popular and powerful tool in recombinant protein expression. When fused to the N- or C-terminus of a target protein, the DYDDDDK tag allows for easy detection, purification, and immunoprecipitation of the expressed protein. Its small size and minimal impact on protein folding and function make it an attractive choice for various research applications. The DYDDDDK tag is recognized by commercially available high-affinity antibodies, facilitating the sensitive and specific detection of the tagged protein using techniques like Western blotting or immunofluorescence. Furthermore, the FLAG tag is amenable to purification via affinity chromatography, where anti-FLAG antibodies are immobilized on a resin, enabling efficient and rapid purification of the tagged protein from complex biological samples. The versatility and efficiency of the DYDDDDK tag have revolutionized the field of molecular biology, aiding researchers in studying protein-protein interactions, subcellular localization, and protein dynamics, ultimately advancing our understanding of cellular processes and driving innovative discoveries in various scientific disciplines. |
Data
 |
| 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2). |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 128 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 36765089 | Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability | Luo Z, Xin D, Liao Y, Berry K, Ogurek S, Zhang F, Zhang L, Zhao C, Rao R, Dong X, Li H, Yu J, Lin Y, Huang G, Xu L, Xin M, Nishinakamura R, Yu J, Kool M, Pfister SM, Roussel MF, Zhou W, Weiss WA, Andreassen P, Lu QR. | Nat Commun. 2023 Feb 10;14(1):762. doi: 10.1038/s41467-023-36400-8. |
| 36685538 | Duck TRIM29 negatively regulates type I IFN production by targeting MAVS | Li W, Song Y, Du Y, Huang Z, Zhang M, Chen Z, He Z, Ding Y, Zhang J, Zhao L, Sun H, Jiao P. | Front Immunol. 2023 Jan 6;13:1016214. doi: 10.3389/fimmu.2022.1016214. eCollection 2022. |
| 36622167 | Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa | Kawalek A, Glabski K, Bartosik AA, Wozniak D, Kusiak M, Gawor J, Zuchniewicz K, Jagura-Burdzy G. | Microbiol Spectr. 2023 Feb 14;11(1):e0428922. doi: 10.1128/spectrum.04289-22. Epub 2023 Jan 9. |
| 36441793 | GluR2Q and GluR2R AMPA Subunits are not Targets of lypd2 Interaction | Lauriello A, McVeigh Q, Sung RJ. | PLoS One. 2022 Nov 28;17(11):e0278278. doi: 10.1371/journal.pone.0278278. eCollection 2022. |
| 35906245 | TRIM28-dependent SUMOylation protects the adult ovary from activation of the testicular pathway | Rossitto M, Déjardin S, Rands CM, Le Gras S, Migale R, Rafiee MR, Neirijnck Y, Pruvost A, Nguyen AL, Bossis G, Cammas F, Le Gallic L, Wilhelm D, Lovell-Badge R, Boizet-Bonhoure B, Nef S, Poulat F. | Nat Commun. 2022 Jul 29;13(1):4412. doi: 10.1038/s41467-022-32061-1. |
Protocols
| relevant to this product |
|---|
| Western blot ICC |
Documents
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