mouse anti-DYDDDDK tag monoclonal antibody (FG4R) 5299

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to DYDDDDK tag
Suitable for: WB,ICC/IF,ELISA
Reacts with: tagged fusion proteins
Isotype: IgG1
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 5299parent Categories: antibody, epitope tag Tag: DYDDDDK tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-DYDDDDK tag monoclonal antibody (FG4R) 5299

antibody
Database link: DYDDDDK tag (FLAG tag)
Tested applications WB,ICC/IF,ELISA
Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Immunogen DYKDDDDK (FLAG) synthetic peptide conjugated to KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions -20°C for 2 years or more. °Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Storage buffer PBS, pH 7.2, 0.05% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
DYDDDDK (Flag) tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify DYDDDDK (Flag) tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, DYDDDDK (Flag) tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody. Click for more on: epitope tags and DYDDDDK (Flag) tag
Protein names FLAG tag, DYKDDDDK, DDDDK tag
Biotechnology The DYDDDDK tag, also known as the FLAG tag, is a short peptide sequence that has become a popular and powerful tool in recombinant protein expression. When fused to the N- or C-terminus of a target protein, the DYDDDDK tag allows for easy detection, purification, and immunoprecipitation of the expressed protein. Its small size and minimal impact on protein folding and function make it an attractive choice for various research applications. The DYDDDDK tag is recognized by commercially available high-affinity antibodies, facilitating the sensitive and specific detection of the tagged protein using techniques like Western blotting or immunofluorescence. Furthermore, the FLAG tag is amenable to purification via affinity chromatography, where anti-FLAG antibodies are immobilized on a resin, enabling efficient and rapid purification of the tagged protein from complex biological samples. The versatility and efficiency of the DYDDDDK tag have revolutionized the field of molecular biology, aiding researchers in studying protein-protein interactions, subcellular localization, and protein dynamics, ultimately advancing our understanding of cellular processes and driving innovative discoveries in various scientific disciplines.

Data

1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2).

Publications

pmid	title	authors	citation
36765089	Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability.	Zaili Luo, Dazhuan Xin, Yunfei Liao, Kalen Berry, Sean Ogurek, Feng Zhang, Liguo Zhang, Chuntao Zhao, Rohit Rao, Xinran Dong, Hao Li, Jianzhong Yu, Yifeng Lin, Guoying Huang, Lingli Xu, Mei Xin, Ryuichi Nishinakamura, Jiyang Yu, Marcel Kool, Stefan M Pfister, Martine F Roussel, Wenhao Zhou, William A Weiss, Paul Andreassen, Q Richard Lu	Nat Commun 14:762
36685538	Duck TRIM29 negatively regulates type I IFN production by targeting MAVS.	Weiqiang Li, Yating Song, Yuqing Du, Zhanhong Huang, Meng Zhang, Zuxian Chen, Zhuoliang He, Yangbao Ding, Junsheng Zhang, Luxiang Zhao, Hailiang Sun, Peirong Jiao	Front Immunol 13:1016214
36622167	Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa.	Adam Kawalek, Krzysztof Glabski, Aneta Agnieszka Bartosik, Dominika Wozniak, Magdalena Kusiak, Jan Gawor, Karolina Zuchniewicz, Grazyna Jagura-Burdzy	Microbiol Spectr 11:e0428922
36441793	GluR2Q and GluR2R AMPA Subunits are not Targets of lypd2 Interaction.	Anna Lauriello, Quinn McVeigh, Rou-Jia Sung	PLoS One 17:e0278278
35906245	TRIM28-dependent SUMOylation protects the adult ovary from activation of the testicular pathway.	Mo�ra Rossitto, Stephanie D�jardin, Chris M Rands, Stephanie Le Gras, Roberta Migale, Mahmoud-Reza Rafiee, Yasmine Neirijnck, Alain Pruvost, Anvi Laetitia Nguyen, Guillaume Bossis, Florence Cammas, Lionel Le Gallic, Dagmar Wilhelm, Robin Lovell-Badge, Brigitte Boizet-Bonhoure, Serge Nef, Francis Poulat	Nat Commun 13:4412

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.