mouse anti-6X His tag monoclonal antibody (HIS.H8) 2966

Name: mouse anti-6X His tag monoclonal antibody (HIS.H8) 2966
SKU: 2966parent
Availability: InStock
Rating: 4.43 (32 reviews)

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$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to 6X His tag
Suitable for: WB,ICC/IF,ELISA
Reacts with: tagged fusion proteins
Isotype: IgG2b
100 µg, 25 µg, 1 mg

available sizes

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SKU: 2966parent Categories: antibody, epitope tag Tag: 6XHis tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-6X His tag monoclonal antibody (HIS.H8) 2966

antibody
Database link: 6x His tag sequence HHHHHH
Tested applications WB,ICC/IF,ELISA
Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Immunogen Highly purified HHHHHH conjugated with KLH (6xHIS)
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions -20°C for 2 years or more. °Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks.
Storage buffer PBS, pH 7.2, 0.05% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG2b - Isotype Control

target relevance

6X His tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify 6X His tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, 6X His tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and 6x His tag

Biotechnology
The 6x Histidine (6x His) tag has become an indispensable tool in both biotechnology and scientific research, particularly in the field of recombinant protein expression and purification. The 6x His tag consists of six consecutive histidine residues genetically fused to a protein of interest. This small peptide sequence offers several advantages, making it highly desirable for the generation of fusion proteins. One of its main benefits is its ability to bind to immobilized metal ions, such as nickel or cobalt, with high affinity. This property allows for efficient and selective purification of the fusion protein through metal affinity chromatography, simplifying the protein purification process and yielding highly pure and concentrated protein samples. Furthermore, the 6His tag also facilitates easy detection and quantification of the fusion protein using anti-His antibodies in techniques like Western blotting. The versatility and simplicity of the 6x His tag have significantly advanced research efforts in protein expression, characterization, and functional analysis, allowing researchers to explore the structure and function of a wide range of proteins in diverse biological systems.

Data

LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).

WB-image-mouse-anti-6X His tag-monoclonal-antibody-HIS.H8-2966

Comparison between anti-His tag (HIS.H8) mAb with 2 different vendor Abs, probed against a standard ladder (Qiagen Cat. No. 34705) containing five different His-tagged proteins. All Ab dilutions are 1:2,000 (0.5µg/mL).

Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).

Publications

pmid	title	authors	citation
36640338	Structural analysis of receptor engagement and antigenic drift within the BA.2 spike protein.	James W Saville, Dhiraj Mannar, Xing Zhu, Alison M Berezuk, Spencer Cholak, Katharine S Tuttle, Faezeh Vahdatihassani, Sriram Subramaniam	Cell Rep 42:111964
36627706	Development of SARS-CoV-2 specific IgG and IgA antibodies in serum and milk with different SARS-COV-2 vaccines in lactating women.	Carolina Lechosa-Mu�iz, Mar�a Paz-Zulueta, Juan Irure-Ventura, Jose Manuel Mendez-Legaza, Roc�o Cuesta Gonz�lez, In�s G�mez-Acebo, Marcos L�pez-Hoyos, Javier Llorca, Mar�a Jes�s Cabero-P�rez	Int Breastfeed J 18:3
36535926	Stabilization of MCL-1 by E3 ligase TRAF4 confers radioresistance.	Ming Li, Feng Gao, Xiaoying Li, Yu Gan, Shuangze Han, Xinfang Yu, Haidan Liu, Wei Li	Cell Death Dis 13:1053
36504569	Evaluation of PLGA-Encapsulated Recombinant GroEL of S. typhi immune Responses Against Enterohaemorrhagic and Enteropathogenic Escherichia coli.	Milad Parvane, Shahram Nazarian, Emad Kordbache, Javad Fathi, Mohamad Ebrahim Minae, Mohammad Reza Ramezani	Avicenna J Med Biotechnol 14:294-302
36494335	SARS-CoV-2 Spike triggers barrier dysfunction and vascular leak via integrins and TGF-? signaling.	Scott B Biering, Francielle Tramontini Gomes de Sousa, Laurentia V Tjang, Felix Pahmeier, Chi Zhu, Richard Ruan, Sophie F Blanc, Trishna S Patel, Caroline M Worthington, Dustin R Glasner, Bryan Castillo-Rojas, Venice Servellita, Nicholas T N Lo, Marcus P Wong, Colin M Warnes, Daniel R Sandoval, Thomas Mandel Clausen, Yale A Santos, Douglas M Fox, Victoria Ortega, Anders M N��r, Ralph S Baric, Sarah A Stanley, Hector C Aguilar, Jeffrey D Esko, Charles Y Chiu, John E Pak, P Robert Beatty, Eva Harris	Nat Commun 13:7630

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.