| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG2b |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 1 mg, 100 µg, 25 µg |
mouse anti-DYDDDDK tag monoclonal antibody (1D2) 8963
$100.00 – $2,600.00
Antibody summary
- Mouse monoclonal to DYDDDDK tag
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG2b
- 100 µg, 25 µg, 1mg
mouse anti-DYDDDDK tag monoclonal antibody (1D2) 8963
| antibody |
|---|
| Database link: DYDDDDK tag (FLAG tag) |
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions user optimized |
| Immunogen Highly purified DYKDDDDK conjugated to KLH |
| Size and concentration 25, 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions 2-8°C |
| Storage buffer PBS, pH 7.2, 0.09% NaN3 |
| Purity affinity purified |
| Clonality monoclonal |
| Isotype IgG2b |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG2b - Isotype Control |
| target relevance |
|---|
| DYDDDDK (Flag) tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify DYDDDDK (Flag) tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, DYDDDDK (Flag) tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody. Click for more on: epitope tags and DYDDDDK (Flag) tag |
| Biotechnology The DYDDDDK tag, also known as the FLAG tag, is a short peptide sequence that has become a popular and powerful tool in recombinant protein expression. When fused to the N- or C-terminus of a target protein, the DYDDDDK tag allows for easy detection, purification, and immunoprecipitation of the expressed protein. Its small size and minimal impact on protein folding and function make it an attractive choice for various research applications. The DYDDDDK tag is recognized by commercially available high-affinity antibodies, facilitating the sensitive and specific detection of the tagged protein using techniques like Western blotting or immunofluorescence. Furthermore, the FLAG tag is amenable to purification via affinity chromatography, where anti-FLAG antibodies are immobilized on a resin, enabling efficient and rapid purification of the tagged protein from complex biological samples. The versatility and efficiency of the DYDDDDK tag have revolutionized the field of molecular biology, aiding researchers in studying protein-protein interactions, subcellular localization, and protein dynamics, ultimately advancing our understanding of cellular processes and driving innovative discoveries in various scientific disciplines. |
Data
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Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot ICC ELISA |
Documents
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