ICC/IF image mouse anti mCherry monoclonal antibody 5A6 6575

mouse anti-mCherry monoclonal antibody (5A6) 6575

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to mCherry
Suitable for: WB, ICC/IF, IHC
Reacts with: tagged fusion proteins
Isotype: IgG1
100 µg, 25 µg, 1 mg

available sizes

Clear

SKU: 6575parent Categories: antibody, epitope tag Tag: mCherry

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

mouse anti-mCherry monoclonal antibody (5A6) 6575

antibody
Database link: mCherry X5DSL3
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:2000 IF/IHC: 1:500
Immunogen Full length recombinant mCherry protein expressed in and purified from E. coli
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance

Protein expression of mCherry and mCherry tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, mCherry fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody.

Click for more on: epitope tags and mCherry

Biotechnology
mCherry, a widely used fluorescent protein, has become an indispensable tool in various areas of scientific research. Derived from the red fluorescent protein DsRed, mCherry emits bright red fluorescence when excited with green light, making it an excellent choice for fluorescence microscopy and live-cell imaging studies. Researchers commonly utilize mCherry as a reporter gene, allowing them to track and visualize specific proteins, organelles, or cellular structures in real-time within living cells and organisms. Its compatibility with other fluorescent proteins enables dual-labeling experiments and multiplexing, providing valuable insights into dynamic cellular processes and interactions. Additionally, mCherry???s stability and resistance to photobleaching enhance its utility for long-term imaging studies. Moreover, mCherry has been employed in various fields, including cell biology, molecular biology, neuroscience, and developmental biology, where its fluorescence serves as a powerful tool to investigate cellular behavior, protein localization, and gene expression patterns. As research methodologies continue to advance, mCherry???s versatility and reliability continue to contribute significantly to our understanding of complex biological phenomena and pave the way for new discoveries in the life sciences.

Data

Immunofluorescent analysis of HEK293 cells stably transduced with a lentiviral vector expressing mCherry-HA construct (red) and stained with mouse mAb to mCherry, 6575, dilution 1:500 in green. The blue is Hoechst staining of nuclear DNA. The 6575 antibody reveals the mCherry protein expressed only in transduced cells which appear golden in color. Untransduced cells expressing no mCherry are not recognized by the 6575 antibody and so only their nuclei are visible.

Western blot analysis of HEK293 whole cell lysates using mouse mAb to mCherry, 6575, dilution 1:2,000, in green [1] protein standard, [2] HEK293, [3] HEK293 cells stably transduced with lenti-virus containing mCherry-HA construct, and [4] HEK293 cells transfected with mCherry-HAI construct. Major band at about 28kDa corresponds to mCherry protein. The band at 20kDa corresponds to cleaved form of mCherry protein. This antibody was made against a recombinant construct expressed in and purified from E. coli. The sequence is identical to that found in a series of widely used expression vectors. Epitope mapping of 6575 was performed using a nested set of 20 amino acid peptides covering the whole sequence and overlapping by 5 amino acids. The peptide highlighted above strongly inhibited binding of 6575 to mCherry, while the previous and following peptides had no effect suggesting that the central 10 amino acids, KLKDGGHYDA are a key component of antibody binding.

Chromogenic Immunostaining of formalin fixed paraffin embedded mCherry transfected monkey brain with mouse mAb to mCherry, 6575, dilution 1:2,000, detected with DAB (brown) following the Vector labs Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. 6575 specifically detected the soma and axons of mCherry positive neurons in the cerebellum, as expected for this model.

Publications

pmid	title	authors	citation
11050231	Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine).	A A Heikal, S T Hess, G S Baird, R Y Tsien, W W Webb	Proc Natl Acad Sci U S A 97:11996-2001
11050230	The structure of the chromophore within DsRed, a red fluorescent protein from coral.	L A Gross, G S Baird, R C Hoffman, K K Baldridge, R Y Tsien	Proc Natl Acad Sci U S A 97:11990-5
11050229	Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral.	G S Baird, D A Zacharias, R Y Tsien	Proc Natl Acad Sci U S A 97:11984-9
10504696	Fluorescent proteins from nonbioluminescent Anthozoa species.	M V Matz, A F Fradkov, Y A Labas, A P Savitsky, A G Zaraisky, M L Markelov, S A Lukyanov	Nat Biotechnol 17:969-73
8303295	Green fluorescent protein as a marker for gene expression.	M Chalfie, Y Tu, G Euskirchen, W W Ward, D C Prasher	Science 263:802-5

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.