Skip to content

chicken anti-mCherry polyclonal antibody 1514

(5 customer reviews)

$100.00$2,600.00

Antibody summary

  • Chicken polyclonal to mCherry
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: tagged fusion proteins
  • Isotype: IgY
  • 100 µg, 25 µg, 1 mg
SKU: 1514parent Categories: , Tags: ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

chicken

isotype

IgY

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

available sizes

1 mg, 100 µg, 25 µg

Available product – chicken anti-mCherry polyclonal antibody 1514

antibody
Database link:
mCherry X5DSL3
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:2000-5000 IF/IHC: 1:1000
Immunogen
Full length recombinant protein expressed in and purified from E. coli.
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
affinity purified
Clonality
polyclonal
Isotype
IgY
Compatible secondaries
goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036
goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317
goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718
goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control
Chicken polyclonal - Isotype Control
target relevance
Protein expression of mCherry and mCherry tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, mCherry fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody.

Click for more on: epitope tags and mCherry
Biotechnology
mCherry, a widely used fluorescent protein, has become an indispensable tool in various areas of scientific research. Derived from the red fluorescent protein DsRed, mCherry emits bright red fluorescence when excited with green light, making it an excellent choice for fluorescence microscopy and live-cell imaging studies. Researchers commonly utilize mCherry as a reporter gene, allowing them to track and visualize specific proteins, organelles, or cellular structures in real-time within living cells and organisms. Its compatibility with other fluorescent proteins enables dual-labeling experiments and multiplexing, providing valuable insights into dynamic cellular processes and interactions. Additionally, mCherry’s stability and resistance to photobleaching enhance its utility for long-term imaging studies. Moreover, mCherry has been employed in various fields, including cell biology, molecular biology, neuroscience, and developmental biology, where its fluorescence serves as a powerful tool to investigate cellular behavior, protein localization, and gene expression patterns. As research methodologies continue to advance, mCherry’s versatility and reliability continue to contribute significantly to our understanding of complex biological phenomena and pave the way for new discoveries in the life sciences.

Data

ICC/IF-image-chicken-anti-mCherry-polyclonal-antibody-1514
HEK293 cells transfected with a mCherry, stained with 1514 antibody and viewed in a confocal microscope. Most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Cells which are transfected with mCherry are bright red, and staining with 1514 is shown in Green. The green antibody staining is only seen cells which express mCherry, as expected, and the superimposition of the green and red results in an orange signal. Interestingly stronger mCherry staining is seen in the nucleus, possibly due to degradation of some mCherry molecules releasing the low molecular weight mCherry fluorochrome.
IHC-image-chicken-anti-mCherry-polyclonal-antibody-1514
Chromogenic immunostaining of formalin fixed paraffin embedded tissue from a mouse transgenically expressing a Cre dependent oxytocin-Tdtomato construct in brain tissue. TdTomato is very closely related to mCherry, so 1514 recognizes both proteins. The 1514 antibody was used at a dilution 1:10,000, detected in DAB (brown) following the 8718C method and citra buffer heat retrieval. Hematoxylin (blue) was used as the counterstain. The 1514 specifically detects as expected the soma and axons of Cre expressing neurons. Mouse select image for larger view.
WB-image-chicken-anti-mCherry-polyclonal-antibody-1514
Western blot analysis of HEK293 cell lysates using chicken pAb to mCherry, 1514, dilution 1:2,000, in green, and rabbit pAb to GAPDH, 574, dilution 1:5,000, in red: [1] protein molecular weight standard, [2] untransfected HEK293 control cells, [3] HEK293 cells transfected with pCI-Neo-mod vector expressing two tdTomato protein domains, [4] HEK293 cells transfected with pCI-Neo-mod vector expressing one mCherry-HA protein domain, and [5] HEK293 cells transfected with pCI-Neo-mod vector expressing one GFP domain. The 1514 antibody recognizes tdTomato and mCherry proteins revealing major bands at about 60kDa and 30kDa, but does not recognize GFP. The red band at 37kDa corresponds to GAPDH protein here used as a loading control.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation
15558047Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent proteinShaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY.Nat Biotechnol. 2004 Dec;22(12):1567-72. doi: 10.1038/nbt1037. Epub 2004 Nov 21.
11050231Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: coral red (dsRed) and yellow (Citrine)Heikal AA, Hess ST, Baird GS, Tsien RY, Webb WW.Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11996-2001. doi: 10.1073/pnas.97.22.11996.
11050230The structure of the chromophore within DsRed, a red fluorescent protein from coralGross LA, Baird GS, Hoffman RC, Baldridge KK, Tsien RY.Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11990-5. doi: 10.1073/pnas.97.22.11990.
11050229Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coralBaird GS, Zacharias DA, Tsien RY.Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):11984-9. doi: 10.1073/pnas.97.22.11984.

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

#
No results found

5 reviews for chicken anti-mCherry polyclonal antibody 1514

  1. verified user

    Review details
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample type:Mouse Tissue sections (brain)
  2. verified user

    Review details
    Immunohistochemistry (Frozen sections)
    Sample type:Mouse Tissue sections (Trigeminal ganglion)
  3. verified user

    Review details
    Immunohistochemistry free floating, Rat Tissue sections (brain, 40 micron thick
    Sample type:sectioned in coronal plane)
  4. verified user

    Review details
    Immunocytochemistry/ Immunofluorescence
    Sample type:Human Cell (H7.S6 Human ES cells with mCherry gene reporter)
  5. verified user

    Review details
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample type:Rat Tissue sections (brain)

Only logged in customers who have purchased this product may leave a review.