| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Chicken IgY H&L Chain |
| available sizes | 1 mg |
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036
$208.00
Antibody summary
- Goat polyclonal to Chicken IgY, H&L Chain Specific-Biotin
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 1 mg
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunohistochemistry: use at a dilution of 1:50-1:500 Immunocytochemistry: use at a dilution of 1:50-1:500. Immunofluorescence: use at a dilution of 1:50-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications. Prep |
| Immunogen Chicken IgY |
| Size and concentration 1mg and 1 mg/mL |
| Form liquid |
| Storage Instructions Store at 2-8°C. Shelf life is one (1) year from date of receipt. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries NA |
| Isotype control NA |
| target relevance |
|---|
| chicken IgY |
| Protein names Chicken immunoglobulin Y |
| Alternative names IgY, Chicken IgY, Gallus gallus IgY, Avian IgY |
| Function Chicken IgY mediates humoral immune responses by specifically recognizing antigens and promoting pathogen neutralization, immune clearance, and protection against infection. |
| Subcellular location Secreted; present in blood plasma, extracellular fluids, and highly concentrated in egg yolk. |
| Structure IgY is composed of two heavy chains and two light chains linked by disulfide bonds. Although functionally analogous to mammalian IgG, the IgY heavy chain contains four constant domains and lacks a classic mammalian hinge region. |
| Description Immunoglobulin Y (IgY) is the major serum antibody of birds, reptiles, and amphibians. In chickens, IgY serves a role analogous to mammalian IgG and is the predominant antibody transferred from the hen to the egg yolk, providing passive immunity to developing offspring. |
| Related entities relationship: evolutionary_related_to; entity: Immunoglobulin G relationship: found_in; entity: Egg yolk |
| Keywords Antibody, Immunoglobulin, IgY, Humoral immunity, Passive immunity |
| Structure Chicken IgY is a tetrameric antibody composed of two heavy chains and two light chains. Unlike mammalian IgG, IgY contains four constant domains and lacks a classical hinge region. |
| Function IgY serves as the principal antibody of birds and mediates antigen recognition, pathogen neutralization, and humoral immune protection. |
| Distribution IgY is found in chicken serum and is actively transported into egg yolk to provide maternal immunity to developing chicks. |
| Advantages IgY antibodies typically do not bind mammalian Fc receptors, rheumatoid factors, or complement components, reducing background interference in many immunoassays. |
| Research relevance Chicken IgY is widely used in ELISA, western blotting, immunohistochemistry, lateral flow assays, passive immunization studies, and antibody production because large quantities can be obtained non-invasively from egg yolks. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What applications has the goat anti-chicken IgY H&L chain specific biotin conjugated antibody been validated for?
This antibody has been tested and is suitable for Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA applications.
What is the recommended dilution range for immunohistochemistry when using this antibody?
For immunohistochemistry, it is recommended to use this antibody at a dilution range of 1:50 to 1:500, though optimal dilutions should be determined by the end user based on their specific experimental conditions.
How should this antibody be stored to maintain stability and activity?
The antibody should be stored refrigerated at 2-8°C and has a shelf life of one year from the date of receipt. It is provided in Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.09% Sodium Azide as a preservative.
What is the host species and clonality of this anti-chicken IgY antibody?
This antibody is a polyclonal antibody raised in goat, with specificity for the heavy and light chains of chicken IgY.
What immunogen was used to generate the goat anti-chicken IgY H&L chain specific biotin conjugated antibody?
The immunogen used to produce this antibody was purified chicken IgY.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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