| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | chicken |
| isotype | IgY |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA |
| reactivity | tagged fusion proteins |
| available sizes | 1 mg, 100 µg, 25 µg |
chicken anti-DYDDDDK tag polyclonal antibody 2933
Price range: $100.00 through $2,600.00
Antibody summary
- Chicken polyclonal to DYDDDDK tag
- Suitable for: ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgY
- 100 µg, 25 µg, 1 mg
chicken anti-DYDDDDK tag polyclonal antibody 2933
| target relevance |
|---|
| DDDDK |
| Protein names FLAG tag |
| Alternative names FLAG tag, FLAG epitope, DYKDDDDK tag, DYDDDDK tag, FLAG peptide |
| Function The FLAG tag serves as an affinity and detection tag that can be fused to recombinant proteins. It is recognized by anti-FLAG antibodies and affinity reagents for purification, immunoprecipitation, western blotting, immunofluorescence, flow cytometry, and ELISA. |
| Description The FLAG tag is a short artificial peptide epitope widely used in recombinant protein expression, purification, detection, and localization studies. The canonical FLAG sequence is DYKDDDDK. |
| Biological role Recombinant protein detection Affinity purification Immunoprecipitation Protein localization studies |
| Research relevance FLAG-tagged proteins are extensively used in molecular biology and protein expression systems because the tag is small, highly immunogenic, and generally does not interfere with protein folding or function. |
| Keywords Epitope tag, Protein purification, Protein detection, Recombinant protein |
| Structure The FLAG tag is a synthetic peptide epitope with the canonical amino acid sequence DYKDDDDK. |
| Function FLAG serves as a molecular tag for detection, purification, and characterization of recombinant proteins. |
| Detection FLAG-tagged proteins are commonly detected using anti-FLAG antibodies such as M1 and M2 clones in western blotting, ELISA, immunofluorescence, and immunoprecipitation assays. |
| Purification FLAG affinity resins and magnetic beads enable rapid purification of FLAG-tagged proteins under native or denaturing conditions. |
| Research relevance The FLAG tag is one of the most widely used epitope tags in molecular biology, biotechnology, and recombinant protein production. |
Data
 |
| Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed, permeabilized with 0.1% triton X-100 in CHO cells transfected with GFP 3xDYKDDDK constructs (CHO-GFP-3XFLAG) labeling DYKDDDDK with chicken anti-DYDDDDK tag polyclonal antibody 2933 (5µg/mL), followed by goat anti-chicken IgY (H&L), Alexa fluor 594 antibody at 2.5µg/mL (red). Nucleus was counterstained with DAPI (blue). Panel below shows CHO parental cell line staining. |
 |
| Negative control - lack of CHO parental cell line antibody staining. |
FAQ & Publications
Frequently Asked Questions
What applications is the chicken anti-DYDDDDK tag polyclonal antibody 2933 validated for?
This antibody is validated for use in ELISA applications to detect DYDDDDK (FLAG) tagged fusion proteins.
How should the chicken anti-DYDDDDK tag polyclonal antibody 2933 be stored to maintain stability?
The antibody should be stored refrigerated at 2-8°C in PBS buffer at pH 7.2 containing 0.09% sodium azide to ensure stability.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| ELISA |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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