chicken anti-GFP polyclonal antibody 1777

$100.00 – $2,600.00

Antibody summary

Chicken polyclonal to GFP
Suitable for: WB, ICC/IF, IHC
Reacts with: tagged fusion proteins
Isotype: IgY
100 µg, 25 µg, 1 mg

SKU: 1777parent Categories: antibody, epitope tag Tag: GFP

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	chicken
isotype	IgY
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
available sizes	1 mg, 100 µg, 25 µg

chicken anti-GFP polyclonal antibody 1777

antibody
Database link: P42212
Tested applications WB,ICC/IF,IHC
Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.
Immunogen Recombinant GFP expressed in and purified from E. coli
Size and concentration 100µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgY
Compatible secondaries goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036 goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317 goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718 goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control Chicken polyclonal - Isotype Control

target relevance
Protein expression of GFP and GFP tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, GFP fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody. Click for more on: epitope tags and GFP
Protein names Green fluorescent protein
Gene names GFP,GFP
Protein family GFP family
Mass 26886Da
Function Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Tissues Photocytes.
Structure Monomer.
Post-translational modification Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnology Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Green fluorescent protein can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when Thr-203 is replaced by Tyr). Further generation of mutants led to more stable proteins (at 37 degrees Celsius for example) with brighter fluorescence and longer fluorescence lifetimes. Fluorescent proteins and their mutated allelic forms have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions (PubMed:17685514, PubMed:17685554, PubMed:8578587, PubMed:8707053, PubMed:9145105, PubMed:9154981, PubMed:9759496, PubMed:9782051). Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy (PubMed:17685514).
Target Relevance information above includes information from UniProt accession: P42212
The UniProt Consortium

Data

https://benchmarkantibodies.com/wp-content/uploads/2024/04/1777_1.jpg

Immunofluorescence cell staining- chicken anti-GFP polyclonal antibody 1777 Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% triton X-100 CHO cells transfected with GFP constructs (CHO-GFP) anti-GFP polyclonal antibody 1777 at 1/100 dilution (10µg/mL), followed by Goat Anti-chicken IgY, Alexa 594 conjugate at 1/400 (5µg/mL) (red). The image also shows GFP (green), nucleus counterstained with DAPI (blue). Also shown lack of staining in parental cell line and from secondary only controls.

https://benchmarkantibodies.com/wp-content/uploads/2024/04/1777_3.jpg

Incubation with secondary antibody only.

https://benchmarkantibodies.com/wp-content/uploads/2024/04/1777_4.jpg

Western blot analysis of chicken anti-GFP polyclonal antibody 1777 CHO cells were transfected with pcDNA3-GFP (as CHO-GFP). THE whole cell lysates were loaded at 2µg and 0.2µg per lane them incubated with chicken anti-GFP polyclonal antibody 1777 at 1:10,000 (01.%micro;g/mL) dilution for 1 hour and followed with anti-chicken IgY HRP conjugate at 1:10,000 (0.1µg/mL) at room temperature.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.