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rabbit anti-beta actin monoclonal antibody 9038

$409.00

Antibody summary

  • Rabbit monoclonal to beta actin
  • Suitable for: WB,ICC/IF,ELISA
  • Reacts with: human, mouse, rat
  • Isotype: IgG
  • 100 µg
SKU: 9038 Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

monoclonal

concentration

1 mg/mL

applications

ELISA, ICC/IF, WB

available sizes

100 µg

rabbit anti-beta actin monoclonal antibody 9038

antibody
Database link:
human P60709
mouse P60710
rat P60711
Tested applications
WB,ICC/IF,ELISA
Recommended dilutions
WB: 1:1000-5000
Size and concentration
100µg and 1 mg/mL
Form
liquid
Storage buffer
PBS, pH 7.2, 0.05% NaN3
Purity
affinity purified
Clonality
monoclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit monocolonal IgG - Isotype Control
target relevance
Actin, a highly conserved protein, is widely recognized as an essential loading control in scientific research, particularly in the field of cell biology and molecular biology. As a structural component of the cytoskeleton, actin is present in virtually all eukaryotic cells. Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Many of these processes are mediated by extensive and intimate interactions of actin with cellular membranes. Its ubiquitous expression and relatively stable levels make it an ideal reference protein for normalizing protein loading in Western blotting and other protein analysis techniques. Actin' abundance and consistent expression ensure that its quantity remains relatively constant across different experimental conditions and sample variations.

Actin isoforms are distinct variants of the actin protein found in eukaryotic cells, and they play crucial roles in various cellular processes. One of the primary differences between actin isoforms lies in their tissue-specific distribution. For instance, skeletal muscle cells predominantly express the α-actin isoform, while smooth muscle cells mainly contain the γ-actin isoform. These tissue-specific distributions are essential for the specialized functions of muscle cells. Moreover, actin isoforms can also differ in their post-translational modifications. For example, γ-actin is more prone to undergo acetylation than α-actin. Additionally, actin isoforms can exhibit variations in their kinetic properties, such as rates of polymerization and depolymerization, which can influence their roles in processes like cell motility or cytoskeletal stability. Furthermore, some actin isoforms may interact with different binding partners or regulatory proteins, further diversifying their functions within the cell. These differences in tissue distribution, post-translational modifications, kinetic properties, and protein interactions contribute to the versatility and specificity of actin isoforms in various cellular processes and underline their importance in maintaining cellular structure and function.

This antibody recognizes the beta actin isoform and can be used as a loading control when run alongside proteins of interest with different and resolvable molecular weights and ideally in combination with antibodies of same host and when using a secondary antibody.

Click for more on: loading controls and beta Actin
Protein names
Actin, cytoplasmic 1 (EC 3.6.4.-) (Beta-actin) [Cleaved into: Actin, cytoplasmic 1, N-terminally processed]
Gene names
ACTB,ACTB
Protein family
Actin family
Mass
41737Da
Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Catalytic activity
Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; Evidence=;
Subellular location
Cytoplasm, cytoskeleton Nucleus Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Structure
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix (PubMed:28604741, PubMed:16685646). Each actin can bind to 4 others (PubMed:28604741, PubMed:16685646). Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs (PubMed:17289661). Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C (PubMed:18765789). In muscle cells, the BAF complex also contains DPF3 (PubMed:18765789). Found in a complex with XPO6, Ran, ACTB and PFN1 (PubMed:14592989). Interacts with XPO6 and EMD (PubMed:15328537). Interacts with ERBB2 (PubMed:21555369). Interacts with GCSAM (PubMed:17823310). Interacts with TBC1D21 (By similarity). Interacts with CPNE1 (via VWFA domain) and CPNE4 (via VWFA domain) (By similarity). Interacts with DHX9 (via C-terminus); this interaction is direct and mediates the attachment to nuclear ribonucleoprotein complexes (PubMed:11687588). Interacts with FAM107A (PubMed:21969592, PubMed:28604741). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The filament contains 8 copies of ACTR1A and 1 ACTB (By similarity).
Post-translational modification
ISGylated. ; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization. ; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes (PubMed:23673617). Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (PubMed:23673617). ; Methylated at His-73 by SETD3 (PubMed:30526847, PubMed:30626964, PubMed:30785395, PubMed:31388018). Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity). ; [Actin, cytoplasmic 1]: N-terminal cleavage of acetylated methionine of immature cytoplasmic actin by ACTMAP. ; [Actin, cytoplasmic 1, N-terminally processed]: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins (PubMed:29581253). In contrast, filament nucleation by the Arp2/3 complex is not affected (PubMed:29581253). ; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
Involvement in disease
Dystonia, juvenile-onset (DJO) [MIM:607371]: A form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. Patients with juvenile-onset dystonia manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. Note=The disease is caused by variants affecting the gene represented in this entry.; Baraitser-Winter syndrome 1 (BRWS1) [MIM:243310]: A rare developmental disorder characterized by the combination of congenital ptosis, high-arched eyebrows, hypertelorism, ocular colobomata, and a brain malformation consisting of anterior-predominant lissencephaly. Other typical features include postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P60709
The UniProt Consortium

Data

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Detection of human beta Actin by western blot. Samples: Whole cell lysate (10 µg) from U2OS, A-549, K-562, HeLa, HEK293T, Jurkat, MOLT-4, and Hep-G2 cells prepared using lysis buffer. Antibody: Affinity purified rabbit anti-beta Actin antibody used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
ICC

Documents

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