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rabbit anti-beta Tubulin monoclonal antibody 9003

$409.00

Antibody summary

  • Rabbit monoclonal to beta Tubulin
  • Suitable for: WB,ICC/IF,ELISA
  • Reacts with: human, mouse, rat
  • Isotype: IgG
  • 100 µg
SKU: 9003 Categories: , Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

rabbit

isotype

IgG

clonality

monoclonal

concentration

1 mg/mL

applications

ELISA, ICC/IF, WB

available sizes

100 µg

Available product – rabbit anti-beta Tubulin monoclonal antibody 9003

antibody
Database link:
human P07437
mouse P99024
rat P69897
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:5000-1:10000, ICC/IF and IHC: 1:1000-1:5000
Immunogen
Pig brain tubulin preparation
Size and concentration
100µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
affinity purified
Clonality
monoclonal
Isotype
IgG
Compatible secondaries
goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863
goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371
goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715
goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control
Rabbit monocolonal IgG - Isotype Control
target relevance
Beta tubulin is a structural component of microtubules, beta-tubulin plays a fundamental role in maintaining cell shape, supporting intracellular transport, and facilitating cell division. Due to its stable expression levels and essential cellular functions, beta-tubulin has become widely recognized as a valuable loading control in various experimental techniques, such as Western blotting and immunoblotting. Its consistent presence in most eukaryotic cells ensures that its expression remains relatively constant, making it an ideal reference protein for normalizing and quantifying target protein levels accurately. This antibody can be used as a loading control when run alongside proteins of interest with different and resolvable molecular weights and ideally in combination with antibodies of same host and when using a secondary antibody.

Click for more on: loading controls and beta Tubulin
Protein names
Tubulin beta chain (Tubulin beta-5 chain)
Gene names
TUBB,TUBB TUBB5 OK/SW-cl.56
Protein family
Tubulin family
Mass
49671Da
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Subellular location
Cytoplasm, cytoskeleton
Tissues
Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Structure
Heterodimer of alpha and beta chains (PubMed:26637975). A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with PIFO (PubMed:20643351). Interacts with DIAPH1 (PubMed:23325789). Interacts with MX1 (By similarity). May interact with RNABP10 (By similarity). Interacts with CFAP157 (By similarity). Nascent tubulin polypeptide interacts (via beta-tubulin MREI motif) with TTC5/STRAP; this interaction results in tubulin mRNA-targeted degradation (PubMed:31727855).
Post-translational modification
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866, PubMed:28576883). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Glutamylation is also involved in cilia motility (By similarity). ; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility (Probable) (PubMed:28576883). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally (Probable) (PubMed:28576883). ; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
Target Relevance information above includes information from UniProt accession : P07437
The UniProt Consortium

Data

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Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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