ICC/IF image mouse anti Hsp60 monoclonal antibody 1C7 8516

mouse anti-Hsp60 monoclonal antibody (1C7) 8516

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to Hsp60
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgG1
100 µg, 25 µg, 1mg

SKU: 8516parent Categories: antibody, loading control Tag: Hsp60

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

mouse anti-Hsp60 monoclonal antibody (1C7) 8516

antibody
Database link: human P10809 mouse P63038 rat P63039
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:10000 ICC/IF and IHC: 1:5000
Immunogen Spontaneous autoantibody, binds LNERLAKLSDGVAVLKVGGT, amino acids 390-409 of the human sequence
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
Heat shock protein 60 (HSP60), also known as chaperonin 60, is a highly conserved molecular chaperone in mitochondria that assists in the folding and assembly of newly synthesized proteins, particularly under conditions of cellular stress. This antibody can be used as a loading control when run alongside proteins, particularly mitochondrail proteins, of interest with different and resolvable molecular weights and ideally in combination with antibodies of same host and when using a secondary antibody. Click for more on: loading controls and HSP60
Protein names 60 kDa heat shock protein, mitochondrial (EC 5.6.1.7) (60 kDa chaperonin) (Chaperonin 60) (CPN60) (Heat shock protein 60) (HSP-60) (Hsp60) (HuCHA60) (Mitochondrial matrix protein P1) (P60 lymphocyte protein)
Gene names HSPD1,HSPD1 HSP60
Protein family Chaperonin (HSP60) family
Mass 61055Da
Function Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
Catalytic activity Reaction=ATP + H2O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide.; EC=5.6.1.7; Evidence=;
Subellular location Mitochondrion matrix.
Structure Homoheptamer arranged in a ring structure (PubMed:1346131, PubMed:11422376, PubMed:25918392). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. Interacts with 2 heptameric Hsp10 rings to form the symmetrical football complex (PubMed:25918392). Interacts with HRAS (By similarity). Interacts with ATAD3A (PubMed:22664726). Interacts with ETFBKMT and EEF1AKMT3 (PubMed:23349634). Interacts with MFHAS1 (PubMed:24286120). ; (Microbial infection) Interacts with hepatitis B virus/HBV protein X. ; (Microbial infection) Interacts with HTLV-1 protein p40tax.
Involvement in disease Spastic paraplegia 13, autosomal dominant (SPG13) [MIM:605280]: A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Note=The disease is caused by variants affecting the gene represented in this entry.; Leukodystrophy, hypomyelinating, 4 (HLD4) [MIM:612233]: A severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound intellectual disability. Death usually occurs within the first two decades of life. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P10809
The UniProt Consortium

Immunofluorescent analysis of HeLa cells stained with mouse mAb to heat shock protein 60 (HSP60), 8516, dilution 1:5,000, in red. Blue is DAPI staining of nuclear DNA. 8516 antibody produces strong and specific staining of mitochondria.

We mapped the epitope for 8516 to within the peptide LNERLAKLSDGVAVLKVGGT, amino acids 390-409 of the human sequence which is highly conserved in all vertebrates so we can predict that 8516 will be widely applicable. See here for full sequence information.. Chromogenic immunostaining of a 4% PFA fixed paraffin embedded rat cerebellum section with mouse mAb to HSP60, 8516, dilution 1:1,000, detected with DAB (brown) using the ImmPress method, with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. This antibody performs well in testing with both 4% PFA and standard NBF fixed tissues but does not stain long term NBF fixed tissue effectively.

Western blot analysis of tissue or whole cell lysates using mouse mAb to HSP60, 8516, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. Strong single band corresponds to HSP60 protein with apparent SDS-PAGE molecular weight of 60kDa.

Publications

pmid	title	authors	citation
35847683	Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimer's Disease Pathology, Constitute a Potential Object for Early Diagnosis.	Gustavo Lopez-Toledo, Maria-Del-Carmen Silva-Lucero, Jorge Herrera-Díaz, David-Erasmo García, José-Antonio Arias-Montaño, Maria-Del-Carmen Cardenas-Aguayo	Front Aging Neurosci 14:921573
22074436	Heat shock protein-60 and risk for cardiovascular disease.	Manfredi Rizzo, Alberto J L Macario, Everly Conway de Macario, Ioanna Gouni-Berthold, Heiner K Berthold, Giovam Battista Rini, Giovanni Zummo, Francesco Cappello	Curr Pharm Des 17:3662-8
14597775	A function for the mitochondrial chaperonin Hsp60 in the structure and transmission of mitochondrial DNA nucleoids in Saccharomyces cerevisiae.	Brett A Kaufman, Jill E Kolesar, Philip S Perlman, Ronald A Butow	J Cell Biol 163:457-61
14585136	Chaperonins are cell-signalling proteins: the unfolding biology of molecular chaperones.	J C Ranford, A R Coates, B Henderson	Expert Rev Mol Med 2:1-17
10604986	Cutting edge: heat shock protein (HSP) 60 activates the innate immune response: CD14 is an essential receptor for HSP60 activation of mononuclear cells.	A Kol, A H Lichtman, R W Finberg, P Libby, E A Kurt-Jones	J Immunol 164:13-7

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.