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mouse anti-Actin (pan) monoclonal antibody (5J11) 7047

$100.00$2,600.00

Antibody summary

  • Mouse monoclonal to Actin (pan)
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: human, mouse, rat, cow, pig
  • Isotype: IgG1
  • 100 µg, 25 µg, 1 mg
SKU: 7047parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

reactivity

cow, human, mouse, pig, rat

available sizes

1 mg, 100 µg, 25 µg

mouse anti-Actin (pan) monoclonal antibody (5J11) 7047

antibody
Database link:
human actins P62736,P63267,P60709,P63261,P68133,P68032
mouse actins P62737P63268,P68033,P60710,P68134,
rat actins P62738,P63268,P63269,P68035,P60711,P68136
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:1000 IF/ICC or IHC: 1:500-1000
Immunogen
Actin preparation from bovine brain
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
affinity purified
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Actin, a highly conserved protein, is widely recognized as an essential loading control in scientific research, particularly in the field of cell biology and molecular biology. As a structural component of the cytoskeleton, actin is present in virtually all eukaryotic cells. Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Many of these processes are mediated by extensive and intimate interactions of actin with cellular membranes. Its ubiquitous expression and relatively stable levels make it an ideal reference protein for normalizing protein loading in Western blotting and other protein analysis techniques. Actin' abundance and consistent expression ensure that its quantity remains relatively constant across different experimental conditions and sample variations.

Actin isoforms are distinct variants of the actin protein found in eukaryotic cells, and they play crucial roles in various cellular processes. One of the primary differences between actin isoforms lies in their tissue-specific distribution. For instance, skeletal muscle cells predominantly express the α-actin isoform, while smooth muscle cells mainly contain the γ-actin isoform. These tissue-specific distributions are essential for the specialized functions of muscle cells. Moreover, actin isoforms can also differ in their post-translational modifications. For example, γ-actin is more prone to undergo acetylation than α-actin. Additionally, actin isoforms can exhibit variations in their kinetic properties, such as rates of polymerization and depolymerization, which can influence their roles in processes like cell motility or cytoskeletal stability. Furthermore, some actin isoforms may interact with different binding partners or regulatory proteins, further diversifying their functions within the cell. These differences in tissue distribution, post-translational modifications, kinetic properties, and protein interactions contribute to the versatility and specificity of actin isoforms in various cellular processes and underline their importance in maintaining cellular structure and function.

This antibody recognizes multiple actin isoforms and can be used as a loading control when run alongside proteins of interest with different and resolvable molecular weights and ideally in combination with antibodies of same host and when using a secondary antibody.

Click for more on: loading controls and Actin
Protein names
Actin, alpha skeletal muscle (EC 3.6.4.-) (Alpha-actin-1) [Cleaved into: Actin, alpha skeletal muscle, intermediate form]
Gene names
ACTA1, ACTA2, ACTB, ACTG1, ACTC1,ACTA1 ACTA2 ACTB ACTG1 ACTC1
Protein family
Actin family
Mass
42051Da
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Catalytic activity
Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; Evidence=;
Subellular location
Cytoplasm, cytoskeleton.
Structure
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Interacts with alpha-actinin (By similarity). Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X (By similarity). Interacts with TTID (PubMed:10958653). Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25m in muscle differentiating cells (PubMed:16501887).
Post-translational modification
Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization. ; Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. ; [Actin, alpha skeletal muscle, intermediate form]: N-terminal cleavage of acetylated cysteine of intermediate muscle actin by ACTMAP. ; Methylated at His-75 by SETD3. ; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
Involvement in disease
Nemaline myopathy 3 (NEM3) [MIM:161800]: A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-like or rod-shaped structures in muscle fibers on histologic examination. Note=The disease is caused by variants affecting the gene represented in this entry.; Myopathy, actin, congenital, with excess of thin myofilaments (MPCETM) [MIM:161800]: A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent. Note=The disease is caused by variants affecting the gene represented in this entry.; Myopathy, congenital, with fiber-type disproportion (CFTD) [MIM:255310]: A genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. Note=The disease is caused by variants affecting the gene represented in this entry.; Myopathy, scapulohumeroperoneal (SHPM) [MIM:616852]: An autosomal dominant muscular disorder characterized by progressive muscle weakness with initial scapulo-humeral-peroneal and distal distribution. Over time, muscle weakness progresses to proximal muscle groups. Clinical characteristics include scapular winging, mild lower facial weakness, foot drop due to foot eversion and dorsiflexion weakness, and selective muscle atrophy. Age at onset and disease progression are variable. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: PANACT
The UniProt Consortium

ICC/IF-image-mouse-anti-Actin (pan)-monoclonal-antibody-5J11-7047
Immunofluorescent analysis of HeLa cells stained with mAb against actin, 7047, in green, costained with EnCor chicken pAb against vimentin, 6886, in red. The actin antibody labels the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites. The vimentin antibody stains a different cytoskeletal network, the intermediate or 10nm filaments. The blue is DAPI staining of nuclear DNA.
IHC-image-mouse-anti-Actin (pan)-monoclonal-antibody-5J11-7047
Chromogenic immunostaining of a formalin fixed paraffin embedded human brain cortex section with mouse mAb to actin, 7047, dilution 1:1,000, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. 7047 antibody labels the cytoplasm of all cell types tested. This antibody performs well in staining both 4% PFA and standard NBF fixed tissues. Mouse select image
WB-image-mouse-anti-Actin (pan)-monoclonal-antibody-5J11-7047
GST-fusion proteins encoding all six full length human actin gene products were run out on SDS-PAGE, transferred to nitrocellulose and reacted with 7047. The 7047 antibody binds all six proteins strongly, so we conclude that it is a pan specific actin antibody. This is not a surprising finding given the high degree of identity between the six proteins, see below. These constructs can be purchased from Novus Biologicals. The HGNC names for the actin isotypes are two α gene products. α-1 actin and α-2, which are enteric and smooth muscle actins (ACTG1 and ACTG2). The single beta-actin is ACTB, and alpha-skeletal actin, alpha-vascular smooth muscle actin and alpha-cardiac muscle are ACTA1, ACTA2, and ACTC1.The amino acid sequences of the six human actin gene products are 94-97% identical, so antibodies which bind all six gene products are to be expected. A sequence alignment can be downloaded from here.
WB-image-mouse-anti-Actin (pan)-monoclonal-antibody-5J11-7047
Western blot analysis of tissue and cell lysates probed with mouse mAb against actin, 7047, in red. [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The same blot was simultaneously probed with EnCor chicken pAb to UCHL-1 , a marker of neuronal lineage cells, in green.

Publications

pmidtitleauthorscitation
37310811Oncostatin-M and OSM-Receptor Feed-Forward Activation of MAPK Induces Separable Stem-like and Mesenchymal ProgramsPolak KL, Tamagno I, Parameswaran N, Smigiel J, Chan ER, Yuan X, Rios B, Jackson MW.Mol Cancer Res. 2023 Sep 1;21(9):975-990. doi: 10.1158/1541-7786.MCR-22-0715.
33839158The C99 domain of the amyloid precursor protein resides in the disordered membrane phaseCapone R, Tiwari A, Hadziselimovic A, Peskova Y, Hutchison JM, Sanders CR, Kenworthy AK.J Biol Chem. 2021 Jan-Jun;296:100652. doi: 10.1016/j.jbc.2021.100652. Epub 2021 Apr 9.
33175436Neuron-specific cilia loss differentially alters locomotor responses to amphetamine in miceRamos C, Roberts JB, Jasso KR, Ten Eyck TW, Everett T, Pozo P, Setlow B, McIntyre JC.J Neurosci Res. 2021 Mar;99(3):827-842. doi: 10.1002/jnr.24755. Epub 2020 Nov 11.
29321224Reconsidering an active role for G-actin in cytoskeletal regulationSkruber K, Read TA, Vitriol EA.J Cell Sci. 2018 Jan 10;131(1):jcs203760. doi: 10.1242/jcs.203760.
27133808Unravelling the Actin Cytoskeleton: A New Competitive Edge?Davidson AJ, Wood W.Trends Cell Biol. 2016 Aug;26(8):569-576. doi: 10.1016/j.tcb.2016.04.001. Epub 2016 Apr 25.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

relevant to this product
Western blot
IHC
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