6X histidine tag antibodies

	species	type			applications
1955	mouse	monoclonal (7B8)	IgG2b		WB			ELISA
2966	mouse	monoclonal (HIS.H8)	IgG2b		WB	ICC		ELISA
7094	rabbit	polyclonal	IgG					ELISA
8278	chicken	polyclonal	IgY					ELISA
9027	goat	polyclonal	IgG		WB	ICC		ELISA

The 6x Histidine (6x His) tag has become an indispensable tool in both biotechnology and scientific research, particularly in the field of recombinant protein expression and purification. The 6x His tag consists of six consecutive histidine residues genetically fused to a protein of interest. This small peptide sequence offers several advantages, making it highly desirable for the generation of fusion proteins. One of its main benefits is its ability to bind to immobilized metal ions, such as nickel or cobalt, with high affinity. This property allows for efficient and selective purification of the fusion protein through metal affinity chromatography, simplifying the protein purification process and yielding highly pure and concentrated protein samples. Furthermore, the 6His tag also facilitates easy detection and quantification of the fusion protein using anti-His antibodies in techniques like Western blotting.