| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | Whole IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | 6 His |
| available sizes | 100 µg |
goat anti-6 His HRP polyclonal antibody 1003
$522.00
Antibody summary
- Goat polyclonal to 6 His , HRP conjugate
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 100 µg
goat anti-6 His HRP polyclonal antibody 1003
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000-1:5,000. Immunocytochemistry: use at a dilution of 1:200-1:500 ELISA: use at a dilution of 1:10,000-1:100,000 These are recommended dilutions. Endusers should determine optimal dilutions for their applications. |
| Immunogen 6-His (HHHHHH) conjugated to KLH. |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| 6X His tag |
| Protein names Hexahistidine tag |
| Alternative names 6xHis tag, His tag, Hexa-His tag, Polyhistidine tag, His6 tag, 6His tag |
| Function The 6×His tag enables purification and detection of recombinant proteins through coordination of histidine residues with immobilized metal ions such as nickel, cobalt, copper, or zinc. |
| Description The 6×His tag is a synthetic peptide tag consisting of six consecutive histidine residues. It is one of the most widely used affinity tags for recombinant protein purification and detection. |
| Related entities relationship: binds_to; entity: Nickel ion relationship: binds_to; entity: Ni-NTA relationship: binds_to; entity: Cobalt affinity resin relationship: modified_form_of; entity: 8×His tag relationship: modified_form_of; entity: 10×His tag |
| Keywords Affinity tag, Protein purification, IMAC, Recombinant protein, Protein detection |
| Sequence HHHHHH |
| Structure The 6×His tag consists of six consecutive histidine residues (HHHHHH) and is typically fused to the N- or C-terminus of recombinant proteins. |
| Function The histidine residues coordinate transition metal ions, enabling affinity purification and detection of tagged proteins. |
| Purification His-tagged proteins are commonly purified using immobilized metal affinity chromatography (IMAC), including Ni-NTA and cobalt-based resins. |
| Detection His-tagged proteins can be detected using anti-His antibodies, anti-polyhistidine antibodies, nickel conjugates, or IMAC reagents. |
| Research relevance The 6×His tag is one of the most widely used recombinant protein tags because of its small size, minimal impact on protein folding, and compatibility with rapid purification workflows. |
Data
 |
| Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, goat anti-6-His antibody 1003 used for WB at 0.2 µg /ml (1:5,000). Detection: Chemiluminescence with an exposure time of 3 minutes. |
FAQ & Publications
Frequently Asked Questions
What applications has the goat anti-6 His HRP polyclonal antibody 1003 been validated for?
This antibody has been tested and validated for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA assays.
What are the recommended dilution ranges for this antibody in different assays?
For immunoblotting, use at a dilution of 1:1,000 to 1:5,000; for immunocytochemistry, use at 1:200 to 1:500; and for ELISA, use at 1:10,000 to 1:100,000. Optimal dilutions should be determined by the end user for specific applications.
How should the goat anti-6 His HRP polyclonal antibody 1003 be stored to maintain stability?
The antibody should be stored at 2-8°C where it remains stable for at least one year. It is supplied in phosphate buffered saline (PBS) containing 0.2% stabilizer.
What is the immunogen used to generate the goat anti-6 His HRP polyclonal antibody 1003?
The immunogen is the 6-His peptide (HHHHHH) conjugated to keyhole limpet hemocyanin (KLH), which induces the production of antibodies specific to the 6 His tag.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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