| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 100 µg |
goat anti-6 His polyclonal antibody 9027
$409.00
Antibody summary
- Goat polyclonal to 6 His
- Suitable for: WB, ICC/IG, IP, ELISA
- Isotype: IgG
- 100 µg
goat anti-6 His polyclonal antibody 9027
| antibody |
|---|
| Database link: 6x His tag sequence HHHHHH |
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions WB: 1:1000-3000 ICC/IF: 1:500-2000 For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test. |
| Immunogen Highly purified HHHHHH conjugated with KLH (6xHIS) |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions -20°C for 2 years or more. °Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for several days to weeks. |
| Storage buffer PBS, pH 7.2, 0.05% NaN3 |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries Donkey anti goat |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| 6X His tag |
| Protein names Hexahistidine tag |
| Alternative names 6xHis tag, His tag, Hexa-His tag, Polyhistidine tag, His6 tag, 6His tag |
| Function The 6×His tag enables purification and detection of recombinant proteins through coordination of histidine residues with immobilized metal ions such as nickel, cobalt, copper, or zinc. |
| Description The 6×His tag is a synthetic peptide tag consisting of six consecutive histidine residues. It is one of the most widely used affinity tags for recombinant protein purification and detection. |
| Related entities relationship: binds_to; entity: Nickel ion relationship: binds_to; entity: Ni-NTA relationship: binds_to; entity: Cobalt affinity resin relationship: modified_form_of; entity: 8×His tag relationship: modified_form_of; entity: 10×His tag |
| Keywords Affinity tag, Protein purification, IMAC, Recombinant protein, Protein detection |
| Sequence HHHHHH |
| Structure The 6×His tag consists of six consecutive histidine residues (HHHHHH) and is typically fused to the N- or C-terminus of recombinant proteins. |
| Function The histidine residues coordinate transition metal ions, enabling affinity purification and detection of tagged proteins. |
| Purification His-tagged proteins are commonly purified using immobilized metal affinity chromatography (IMAC), including Ni-NTA and cobalt-based resins. |
| Detection His-tagged proteins can be detected using anti-His antibodies, anti-polyhistidine antibodies, nickel conjugates, or IMAC reagents. |
| Research relevance The 6×His tag is one of the most widely used recombinant protein tags because of its small size, minimal impact on protein folding, and compatibility with rapid purification workflows. |
Data
 |
| Lysate of E.coli expressing recombinant his-tagged protein |
FAQ & Publications
Frequently Asked Questions
What applications is the Goat anti-6 His polyclonal antibody 9027 validated for, and what are the recommended dilutions?
This antibody is validated for Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA. Recommended dilutions are 1:1000-3000 for WB and 1:500-2000 for ICC/IF. For other applications such as Dot blot, IP, or ELISA, optimal dilutions should be determined by titration.
How should the Goat anti-6 His polyclonal antibody 9027 be stored to maintain its stability and functionality?
The antibody should be stored at -20°C for long-term storage up to 2 years or more. After the first thaw, centrifuge to maximize product recovery and aliquot to avoid repeated freeze-thaw cycles. Aliquots can be stored at 4°C for several days to weeks. The antibody is supplied in PBS buffer, pH 7.2, with 0.05% sodium azide.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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